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Deng, B., Li, Q., Liu, X., Cao, Y., Li, B., Qian, Y., Xu, R., Mao, R., Zhou, E., Zhang, W., Huang, J., Rao, Y. (2019). Chemoconnectomics: Mapping Chemical Transmission in Drosophila.  Neuron 101(5): 876--893.e4.
FlyBase ID
FBrf0241737
Publication Type
Research paper
Abstract

We define the chemoconnectome (CCT) as the entire set of neurotransmitters, neuromodulators, neuropeptides, and their receptors underlying chemotransmission in an animal. We have generated knockout lines of Drosophila CCT genes for functional investigations and knockin lines containing Gal4 and other tools for examining gene expression and manipulating neuronal activities, with a versatile platform allowing genetic intersections and logic gates. CCT reveals the coexistence of specific transmitters but mutual exclusion of the major inhibitory and excitatory transmitters in the same neurons. One neuropeptide and five receptors were detected in glia, with octopamine β2 receptor functioning in glia. A pilot screen implicated 41 genes in sleep regulation, with the dopamine receptor Dop2R functioning in neurons expressing the peptides Dilp2 and SIFa. Thus, CCT is a novel concept, chemoconnectomics a new approach, and CCT tool lines a powerful resource for systematic investigations of chemical-transmission-mediated neural signaling circuits underlying behavior and cognition.

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Related Publication(s)
Personal communication to FlyBase

Rao lab chemoconnectome stocks.
Rao and Deng, 2019.10.23, Rao lab chemoconnectome stocks. [FBrf0243924]

KOGal4 mutant alleles used in sleep analysis experiments in FBrf0241737.
Deng, 2020.1.10, KOGal4 mutant alleles used in sleep analysis experiments in FBrf0241737. [FBrf0244536]

Note

The Excitatory, the Inhibitory, and the Modulatory: Mapping Chemical Neurotransmission in the Brain.
Boto and Tomchik, 2019, Neuron 101(5): 763--765 [FBrf0241806]

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    Language of Publication
    English
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    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Neuron
    Title
    Neuron
    Publication Year
    1988-
    ISBN/ISSN
    0896-6273
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