FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Insertion: Dmel\P{GawB}Ckac305a
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General Information
Symbol
Dmel\P{GawB}Ckac305a
Species
D. melanogaster
Name
FlyBase ID
FBti0100741
Feature type
transposable_element_insertion_site
Also Known As
c305a-GAL4, c305a, c305-Gal4, c305aGAL4, GAL4-c305a, C305
Description
Inserted Element
P{GawB}
LINE ID
Affected gene(s)
Cka, Scer\GAL4
Viability / fertility
Associated allele(s)
Ckac305a, Scer\GAL4c305a
Stock availability
1 publicly available
Genomic Location
Chromosomal Location
2L ( 28D3 )
Sequence Location
2L:8,031,086..8,031,086 [-]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
Description
Detailed Mapping Data
Chromosome (arm)

2

(Cook, 2010.7.28)
Sequence Location
2L:8,031,086..8,031,086 [-]
(Armstrong et al., 2006)
Orientation

-

(Armstrong et al., 2006)
Cytological location (computed by FlyBase)
28D3 (inferred by FlyBase from sequence location)
Cytological location (reported)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct
P{GawB}
(FlyBase, 1992-)
Size (Kb)

11.279

Component Allele(s)
Scer\GAL4wB
w+mW.hs
Molecular map
Affected Gene(s)
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
core layer of the larval mushroom body
(Pauls et al., 2010)
wandering third instar larval stage
neuron of adult mushroom body alpha'-lobe

Comment: reported as 4 days after hatching

(Lin et al., 2013)
neuron of adult mushroom body beta'-lobe

Comment: reported as 4 days after hatching

(Lin et al., 2013)
adult stage
adult antennal lobe
(Aso et al., 2009, Krashes et al., 2007)
ellipsoid body | restricted
(Krashes et al., 2007)
adult antennal nerve | restricted
(Krashes et al., 2007)
pedunculus of adult mushroom body | distal | restricted
(Mao and Davis, 2009)
alpha'/beta' Kenyon cell
(Christiansen et al., 2011, Buchanan and Davis, 2010)
neuron | subset of adult brain
(Chen et al., 2012)
adult mushroom body

Comment: medium expression

(Chen et al., 2012)
glial cell of adult brain

Comment: faint expression

(Chen et al., 2012)
ellipsoid body

Comment: medium expression

(Chen et al., 2012, Aso et al., 2009)
eye

Comment: faint expression

(Chen et al., 2012)
adult mushroom body beta'-lobe
(Riemensperger et al., 2013, Pauls et al., 2010, Tan et al., 2010, Mao and Davis, 2009, Krashes et al., 2007)
adult mushroom body alpha'-lobe
(Riemensperger et al., 2013, Pauls et al., 2010, Tan et al., 2010, Krashes et al., 2007)
mushroom body alpha' middle layer
(Aso et al., 2009)
mushroom body beta' middle layer
(Aso et al., 2009)
mushroom body alpha' anterior-posterior layer | intense
(Aso et al., 2009)
mushroom body beta' anterior-posterior layer | intense
(Aso et al., 2009)
medulla | faint
(Aso et al., 2009)
adult protocerebrum | restricted
(Aso et al., 2009)
inferior neuropils
(Aso et al., 2009)
adult antennal nerve | intense
(Aso et al., 2009)
adult tritocerebrum
(Aso et al., 2009)
adult gnathal ganglion
(Aso et al., 2009)
adult pars intercerebralis | faint
(Aso et al., 2009)
adult stage | male
adult mushroom body alpha'-lobe
(Blum et al., 2009)
adult mushroom body beta'-lobe
(Blum et al., 2009)
ellipsoid body
(Blum et al., 2009)
antennal lobe
(Blum et al., 2009)
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult antennal lobe local neuron
(Melnattur et al., 2013)
Additional Information
Statement
Reference

Drives expression in alpha' and beta' lobes of the mushroom body, and in alpha'/beta' mushroom body neurons.

(FlyBase Curators, 2017)

ScerGAL4c305a labels a large cluster of excitatory local interneurons with cell bodies located lateral to the antennal lobe. Their axons fasciculate and enter the antennal lobe.

(Melnattur et al., 2013)

Expression of ScerGAL4c305a was observed in the alpha' and beta' lobes of the mushroom body at 4 days after larval hatching (late wandering third instar), but not earlier at 2 days after hatching (early third instar).

(Lin et al., 2013)

ScerGAL4c305a labels the mushroom body alpha'/beta' neurons. Co-expression with a postsynaptic marker labels Kenyon cell terminals in the mushroom body alpha' and beta' lobes. No presynaptic terminals in the calyx are observed.

(Christiansen et al., 2011)

This line labels larval-born alpha' and beta' Kenyon cells in the third instar as well as elements in the thoracic and abdominal ganglia, mostly sensory terminals from the periphery.

(Pauls et al., 2010)

Expression is observed in about half of the the alpha', and beta' neurons of the adult mushroom body, in ellipsoid body neurons and in the antennal lobe.

(Blum et al., 2009)

Expression of ScerGAL4c305a in the adult antennal nerve is in putative mechanosensory neurons, and within the ring neurons of the ellipsoid body.

(Krashes et al., 2007)
 
Marker for
adult mushroom body alpha'-lobe
(Krashes et al., 2007)
adult mushroom body beta'-lobe
(Krashes et al., 2007)
alpha'/beta' Kenyon cell
(Christiansen et al., 2011)
Reflects expression of
Reporter construct used in assay
Nf1UAS.cBa
(Buchanan and Davis, 2010)
gishS.UAS
(Tan et al., 2010)
gishL.UAS
(Tan et al., 2010)
Mmus\Cd8aUAS.cLa.EGFP
(Lin et al., 2013, Melnattur et al., 2013, Pauls et al., 2010, Aso et al., 2009, Blum et al., 2009, Mao and Davis, 2009, Krashes et al., 2007)
shi1.UAS
(Krashes et al., 2007)
Scer\GAL80Mef2.mb247
(Krashes et al., 2007)
Disc\RFPmCh.UAS.Tag:TS(brpS)
(Christiansen et al., 2011)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (23)
Reported As
Symbol Synonym
C-305a-GAL4
(Bertin et al., 2022)
C305
(Pauls et al., 2010)
C305A-Gal4
(Bertin et al., 2022)
C305a
(Miyashita et al., 2018, Shuai et al., 2010, Blum et al., 2009)
C305a-GAL4
(Al-Anzi and Zinn, 2018, Sun et al., 2018, Yang et al., 2016)
C305a-Gal4
(Wu et al., 2021, Yatsenko et al., 2021, Xie et al., 2013)
C305a-gal4
(Noyes and Davis, 2023)
C305α-Gal4
(Yang et al., 2021)
Ckac305a-Gal4
(Lancaster et al., 2024)
GAL4-c305
(Marchetti and Tavosanis, 2017)
GAL4-c305a
(Chu et al., 2024, Lin et al., 2009, Lin et al., 2007)
P{GAL4}c305a
(FlyBase, 1992-)
P{GawB}Ckac305a
P{GawB}c305a
(Cook, 2010.7.28)
c305
(Papanikolopoulou et al., 2019)
c305-GAL4
(Bouzaiane et al., 2015)
c305-Gal4
(Valadas et al., 2018, Krüttner et al., 2015, Shankar et al., 2015, Cavaliere et al., 2013)
c305a
(Rozenfeld and Parnas, 2024, Chen et al., 2023, Inami et al., 2021, Sabandal et al., 2021, Deng et al., 2019, Marmor-Kollet et al., 2019, Papanikolopoulou et al., 2019, Louis et al., 2018, Jiang et al., 2016, Scholz-Kornehl and Schwärzel, 2016, Tong et al., 2016, Lewis et al., 2015, Dunne et al., 2014, Lin et al., 2014, Thran et al., 2013, Ueno et al., 2013, Chen et al., 2012, Keleman et al., 2012, Scheunemann et al., 2012, Lee et al., 2011, Hekmat-Scafe et al., 2010, Pauls et al., 2010, Pauls et al., 2010, Tan et al., 2010, Aso et al., 2009, van Swinderen et al., 2009, Krashes et al., 2007, Armstrong et al., 2006)
c305a-GAL4
(Schwartz et al., 2023, Shen et al., 2023, Manoim et al., 2022, Sabandal et al., 2022, Kim et al., 2021, Sabandal et al., 2020, Wu et al., 2020, Bielopolski et al., 2019, Brown et al., 2019, Sengupta et al., 2019, Aranda et al., 2017, Ries et al., 2017, Saras et al., 2017, Drago and Davis, 2016, Liu et al., 2016, Bai and Sehgal, 2015, Haynes et al., 2015, Reynaud et al., 2015, Boto et al., 2014, Vogt et al., 2014, Fitzsimons et al., 2013, Lin et al., 2013, Melnattur et al., 2013, Perrat et al., 2013, Christiansen et al., 2011, Leiss et al., 2009, Mao and Davis, 2009, Keleman et al., 2007)
c305a-Gal4
(Kaldun et al., 2025, Delescluse et al., 2024, Draper et al., 2024, Lancaster et al., 2024, Modi et al., 2023, Foka et al., 2022, Grover et al., 2022, Hatch et al., 2021, Silva et al., 2020, Higham et al., 2019, Jia et al., 2019, Marmor-Kollet et al., 2019, Sun et al., 2018, Widmer et al., 2018, Widmer et al., 2018, Kelly et al., 2016, Chen et al., 2015, Kirkhart and Scott, 2015, Siegenthaler et al., 2015, Andlauer et al., 2014, Malik et al., 2013, Pech et al., 2013, Riemensperger et al., 2013, Zhang et al., 2013, Butcher et al., 2012, Kucherenko et al., 2012)
c305a-gal4
(Roselli et al., 2025, Cervantes-Sandoval et al., 2016, Gai et al., 2016, Tonoki and Davis, 2012, Buchanan and Davis, 2010)
c305aGAL4
(Peng et al., 2024, Hamid et al., 2021, Hamid et al., 2019, Roussou et al., 2019, Agrawal and Hasan, 2015)
c305a{GAL4}
(Krashes et al., 2007)
Name Synonyms
Secondary FlyBase IDs
    References (114)