FlyBase Gene Report: Dmel\nAChRβ2

General Information

Symbol

Dmel\nAChRβ2

Species

D. melanogaster

Name

nicotinic Acetylcholine Receptor β2

Annotation Symbol

CG6798

Feature Type

protein_coding_gene

FlyBase ID

FBgn0004118

Gene Model Status

Current

Stock Availability

29 publicly available

Gene Summary

nicotinic Acetylcholine Receptor β2 (nAChRβ2) encodes a subunit of the nicotinic acetylcholine receptor, which is part of the superfamily of ligand-gated ion channels. These channels open to facilitate the passage of cations across synaptic membranes in response to the binding of their native ligand, acetylcholine. Mutations in nAChRβ2 have been associated with resistance phenotypes to several neonicotinoid insecticides. [Date last reviewed: 2018-09-06] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

SBD, Dβ2, nAcRbeta-96A, nAChR, second beta-like subunit of Drosophila

Key Links

Genomic Location

Cytogenetic map

96A5-96A5

Sequence location

3R:24,509,228..24,515,106 [-]

Recombination map

3-85

RefSeq locus

NT_033777 REGION:24509228..24515106

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (17 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

contributes_to acetylcholine-gated monoatomic cation-selective channel activity

inferred from biological aspect of ancestor with PANTHER:PTN001054960

(GO Reference Genome Project, 2011-)

enables acetylcholine-gated monoatomic cation-selective channel activity

inferred from electronic annotation with InterPro:IPR002394

(InterPro Project Members, 2004-)

enables extracellular ligand-gated monoatomic ion channel activity

inferred from electronic annotation with InterPro:IPR006202, InterPro:IPR036734

(InterPro Project Members, 2004-)

enables monoatomic ion channel activity

inferred from electronic annotation with InterPro:IPR002394, InterPro:IPR006201

(InterPro Project Members, 2004-)

enables neurotransmitter receptor activity

inferred from biological aspect of ancestor with PANTHER:PTN000434994

(GO Reference Genome Project, 2011-)

enables transmembrane signaling receptor activity

inferred from electronic annotation with InterPro:IPR006201

(InterPro Project Members, 2004-)

Biological Process (6 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in response to insecticide

inferred from mutant phenotype

(Perry et al., 2008)

involved_in synaptic transmission, cholinergic

inferred from expression pattern

(Takemura et al., 2011)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

involved_in calcium ion transport

inferred from biological aspect of ancestor with PANTHER:PTN000434994

(GO Reference Genome Project, 2011-)

involved_in monoatomic ion transmembrane transport

inferred from biological aspect of ancestor with PANTHER:PTN000434994

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR006201

(InterPro Project Members, 2004-)

involved_in monoatomic ion transport

inferred from electronic annotation with InterPro:IPR002394, InterPro:IPR006029, InterPro:IPR006202, InterPro:IPR036719, InterPro:IPR036734

(InterPro Project Members, 2004-)

involved_in regulation of membrane potential

inferred from biological aspect of ancestor with PANTHER:PTN000434994

(GO Reference Genome Project, 2011-)

involved_in synaptic transmission, cholinergic

inferred from biological aspect of ancestor with PANTHER:PTN001054960

(GO Reference Genome Project, 2011-)

Cellular Component (6 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (6 terms)

CV Term

Evidence

References

part_of acetylcholine-gated channel complex

inferred from biological aspect of ancestor with PANTHER:PTN000435923

(GO Reference Genome Project, 2011-)

located_in membrane

inferred from electronic annotation with InterPro:IPR002394, InterPro:IPR006029, InterPro:IPR006201, InterPro:IPR006202, InterPro:IPR036719, InterPro:IPR036734

(InterPro Project Members, 2004-)

is_active_in neuron projection

inferred from biological aspect of ancestor with PANTHER:PTN000434994

(GO Reference Genome Project, 2011-)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN001054635

(GO Reference Genome Project, 2011-)

located_in postsynaptic membrane

inferred from electronic annotation with InterPro:IPR002394

(InterPro Project Members, 2004-)

is_active_in synapse

inferred from biological aspect of ancestor with PANTHER:PTN000434994

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

NICOTINIC ACETYLCHOLINE RECEPTOR SUBUNITS

Protein Family (UniProt)

Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. (P25162)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

NICOTINIC ACETYLCHOLINE RECEPTOR SUBUNITS -: Nicotinic ligand-gated ion channels (nAChR) mediate the fast actions of the acetylcholine (ACh). The non-selective ion channel is formed from five subunits arranged around a central pore. The ACh binding site is formed by two adjacent α subunits. Other regulatory subunits may modulate channel pharmacology and expression. (Adapted from FBrf0183743).

Protein Function (UniProtKB)

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

(UniProt, P25162)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\nAChRβ2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P25162)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.47

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

nAChRβ2-RA

FBtr0084681

NM_079759

1892

519

nAChRβ2-RB

FBtr0084682

NM_170147

4018

304

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

nAChRβ2-PA

FBpp0084061

60.1

519

4.61

FBpp0084062

35.6

304

4.56

H5V857

NP_733002

AAN13998

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\nAChRβ2 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

nAChRbeta2_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.46

Transcript Expression

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

transmedullary neuron Tm2

(Takemura et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

medulla | restricted

(Chamaon et al., 2002)

lobula

(Chamaon et al., 2002)

lobula plate

(Chamaon et al., 2002)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

nAcRβ-96A protein is expressed in the lobula, lobula plate, and medulla of the adult optic lobe. There is no expression in the lamina. The medulla is strongly labled in layers 1, 2, 4, 6, and 9; labeling is less intense in layers 3,5,8, and 10, and absent in layer 7.

(Chamaon et al., 2002)

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\nAChRβ2 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\nAChRβ2

View exonic expression by tissue for Dmel\nAChRβ2

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42920/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0004118

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0004118

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0004118

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6798

Flygut - An atlas of the Drosophila adult midgut

FBgn0004118

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 21 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 11 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of nAChRβ2

Transgenic constructs containing regulatory region of nAChRβ2

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(3R)Exel7357

(Ryder, 2004.4.26)

Df(3R)Exel8178

(Ryder, 2004.4.26)

Inferred from location ( 6 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

nAChRβ2^MI02206

viable

nAChRβ2^KO

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

nAChRβ2^GD237

viable, with Scer\GAL4^Mef2.PR

nAChRβ2^GD237

viable, with Scer\GAL4^pnr-MD237

nAChRβ2^GD237

viable, with Scer\GAL4^tin.CΔ4

nAChRβ2^GD237

Other Phenotypes

Allele

abnormal flight, with Scer\GAL4^Mef2.PR

nAChRβ2^GD237

abnormal sleep | adult stage

nAChRβ2^attP

chemical resistant

visible, with Scer\GAL4^pnr-MD237

nAChRβ2^GD237

Phenotype manifest in

Allele

chaeta | increased number, with Scer\GAL4^pnr-MD237

nAChRβ2^GD237

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (27)

Hsap\CHRNA3

12 of 14

Yes

Hsap\CHRNA6

12 of 14

Yes

Hsap\CHRNA2

11 of 14

Yes

Hsap\CHRNA4

9 of 14

Yes

Hsap\CHRNA5

5 of 14

Yes

Hsap\CHRNB3

5 of 14

Yes

Hsap\CHRNA1

4 of 14

Yes

Hsap\CHRNB2

4 of 14

Hsap\CHRNB4

4 of 14

Yes

Hsap\CHRNA9

2 of 14

Hsap\CHRNA10

2 of 14

Hsap\CHRNB1

2 of 14

Yes

Hsap\CHRND

2 of 14

Yes

Hsap\HTR3A

2 of 14

Yes

Hsap\HTR3B

2 of 14

Yes

Hsap\HTR3C

2 of 14

Yes

Hsap\HTR3D

2 of 14

Yes

Hsap\HTR3E

2 of 14

Yes

Hsap\ZACN

2 of 14

Yes

Hsap\CHRFAM7A

1 of 14

Hsap\CHRNA7

1 of 14

Hsap\CHRNE

1 of 14

Yes

Hsap\CHRNG

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (18)

Rnor\Chrna3

12 of 14

Yes

Rnor\Chrna6

12 of 14

Yes

Rnor\Chrna2

11 of 14

Yes

Rnor\Chrna4

8 of 14

Yes

Rnor\Chrnb3

6 of 14

Yes

Rnor\Chrna5

5 of 14

Yes

Rnor\Chrna1

4 of 14

Yes

4 of 14

2 of 14

2 of 14

2 of 14

Yes

Rnor\Chrnb4

2 of 14

Rnor\Chrnd

2 of 14

Yes

Rnor\Chrne

2 of 14

Yes

Rnor\Htr3a

2 of 14

Yes

Rnor\Htr3b

2 of 14

Yes

Rnor\Chrna7

1 of 14

Rnor\Chrng

1 of 14

Yes

Mus musculus (laboratory mouse) (18)

Mmus\Chrna3

12 of 14

Yes

Mmus\Chrna6

12 of 14

Yes

Mmus\Chrna2

11 of 14

Yes

Mmus\Chrna4

8 of 14

Yes

Mmus\Chrna5

5 of 14

Yes

Mmus\Chrnb3

5 of 14

Yes

Mmus\Chrna1

4 of 14

Yes

Mmus\Chrnb2

4 of 14

Mmus\Chrnb4

4 of 14

Yes

Mmus\Chrna9

2 of 14

Mmus\Chrna10

2 of 14

Mmus\Chrnb1

2 of 14

Yes

Mmus\Chrnd

2 of 14

Yes

Mmus\Chrne

2 of 14

Yes

Mmus\Chrng

2 of 14

Yes

Mmus\Htr3a

2 of 14

Yes

Mmus\Htr3b

2 of 14

Yes

Mmus\Chrna7

1 of 14

Xenopus tropicalis (Western clawed frog) (25)

Xtro\chrna2

9 of 13

Yes

Xtro\chrna6

6 of 13

Xtro\LOC101732933

6 of 13

Yes

Xtro\chrna4

5 of 13

Yes

Xtro\chrnb3

3 of 13

Yes

Xtro\chrna1.2

2 of 13

Yes

Xtro\chrna1

2 of 13

Yes

Xtro\chrna5

2 of 13

Yes

Xtro\chrnb2

2 of 13

Xtro\chrnb4

2 of 13

Xtro\gjb7

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Danio rerio (Zebrafish) (32)

Drer\chrna2a

12 of 14

Yes

Drer\chrna6

12 of 14

Yes

Drer\chrna2b

11 of 14

Yes

Drer\chrna3

11 of 14

Drer\chrna4b

9 of 14

Yes

Drer\chrnb3a

5 of 14

Yes

Drer\chrna1

4 of 14

Yes

Drer\chrna5

4 of 14

Yes

Drer\chrnb3b

4 of 14

Yes

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

Yes

Drer\chrnb5b

2 of 14

Drer\chrnd

2 of 14

Yes

Drer\chrne

2 of 14

Yes

Drer\chrng

2 of 14

Yes

Drer\htr3a

2 of 14

Yes

Drer\LOC110439304

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\LOC798522

1 of 14

Drer\LOC100334841

1 of 14

Drer\LOC100537401

1 of 14

Yes

Drer\LOC101882451

1 of 14

Drer\LOC101882637

1 of 14

Drer\si:ch211-256e16.4

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (64)

Cele\unc-63

11 of 14

Yes

Cele\unc-38

10 of 14

Cele\acr-8

5 of 14

Cele\acr-12

5 of 14

Yes

Cele\lev-8

5 of 14

Yes

Cele\acr-6

4 of 14

Yes

Cele\acr-2

3 of 14

Cele\acr-3

3 of 14

Cele\acr-5

3 of 14

Yes

Cele\acr-23

3 of 14

Yes

Cele\cup-4

3 of 14

Yes

Cele\deg-3

3 of 14

Yes

Cele\lgc-24

3 of 14

Yes

Cele\lgc-25

3 of 14

Yes

Cele\lgc-26

3 of 14

Yes

Cele\unc-29

3 of 14

Cele\acr-18

2 of 14

Cele\acr-20

2 of 14

Yes

Cele\acr-21

2 of 14

Yes

Cele\acr-24

2 of 14

Yes

Cele\des-2

2 of 14

Yes

Cele\lev-1

2 of 14

Cele\lgc-1

2 of 14

Cele\lgc-4

2 of 14

Yes

Cele\lgc-5

2 of 14

Yes

Cele\lgc-6

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Cele\lgc-21

2 of 14

Yes

Cele\lgc-23

2 of 14

Yes

Cele\lgc-28

2 of 14

Yes

Cele\lgc-29

2 of 14

Cele\lgc-30

2 of 14

Yes

Cele\pbo-5

2 of 14

Yes

Cele\pbo-6

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\lgc-27

1 of 14

Yes

Cele\lgc-31

1 of 14

Yes

Cele\lgc-48

1 of 14

Cele\lgc-52

1 of 14

Anopheles gambiae (African malaria mosquito) (17)

Agam\AgaP_AGAP002971

11 of 12

Yes

6 of 12

6 of 12

5 of 12

5 of 12

3 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:nAChRβ2. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (22)

11 of 13

11 of 13

11 of 13

9 of 13

5 of 13

4 of 13

3 of 13

3 of 13

3 of 13

NtR

3 of 13

CG6927

2 of 13

GluClα

2 of 13

Grd

2 of 13

Lcch3

2 of 13

pHCl-2

2 of 13

Rdl

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

ort

1 of 13

pHCl-1

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 6 )

Human Ortholog

Disease

Evidence

References

CHRNA2; cholinergic receptor nicotinic alpha 2 subunit

model of autosomal dominant nocturnal frontal lobe epilepsy 4

IEA

(FlyBase, 2019-)

CHRNA4; cholinergic receptor nicotinic alpha 4 subunit

model of autosomal dominant nocturnal frontal lobe epilepsy 1

IEA

(FlyBase, 2019-)

CHRNA1; cholinergic receptor nicotinic alpha 1 subunit

model of contractures, pterygia, and spondylocarpotarsal fusion syndrome

IEA

(FlyBase, 2019-)

model of congenital myasthenic syndrome 1A

IEA

(FlyBase, 2019-)

model of congenital myasthenic syndrome 1B

IEA

(FlyBase, 2019-)

CHRND; cholinergic receptor nicotinic delta subunit

model of contractures, pterygia, and spondylocarpotarsal fusion syndrome

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

CHRNA3; cholinergic receptor nicotinic alpha 3 subunit

12 of 14

118503

CHRNA6; cholinergic receptor nicotinic alpha 6 subunit

12 of 14

606888

CHRNA2; cholinergic receptor nicotinic alpha 2 subunit

11 of 14

118502

EPILEPSY, NOCTURNAL FRONTAL LOBE, 4; ENFL4

autosomal dominant nocturnal frontal lobe epilepsy 4

CHRNA4; cholinergic receptor nicotinic alpha 4 subunit

9 of 14

118504

autosomal dominant nocturnal frontal lobe epilepsy 1

CHRNA5; cholinergic receptor nicotinic alpha 5 subunit

5 of 14

118505

SMOKING AS A QUANTITATIVE TRAIT LOCUS 3; SQTL3

CHRNB3; cholinergic receptor nicotinic beta 3 subunit

5 of 14

118508

CHRNA1; cholinergic receptor nicotinic alpha 1 subunit

4 of 14

100690

CHRNB2; cholinergic receptor nicotinic beta 2 subunit

4 of 14

118507

EPILEPSY, NOCTURNAL FRONTAL LOBE, 3; ENFL3

autosomal dominant nocturnal frontal lobe epilepsy 3

CHRNB4; cholinergic receptor nicotinic beta 4 subunit

4 of 14

118509

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

nAChRβ2 - nAChRβ3

anti tag coimmunoprecipitation, molecular weight estimation by autoradiography

(Lansdell and Millar, 2002)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0004118

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

gene_with_edited_transcript

(Rodriguez et al., 2012)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-85

Cytogenetic map

96A5-96A5

Sequence location

3R:24,509,228..24,515,106 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

96A5-96A5

Limits computationally determined from genome sequence between P{PZ}crb⁰⁷²⁰⁷&P{PZ}BRWD3⁰⁵⁸⁴² and P{EP}CycB3^EP3127

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

96A3-96A3

(determined by in situ hybridisation)

(Ranz et al., 1999, Sawruk et al., 1990)

96A-96A

(determined by in situ hybridisation)

(Gundelfinger, 1992)

Experimentally Determined Recombination Data

Location

3-85

(FlyBase, 2016)

3-83.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (29)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

84666

TI{2A-GAL4}nAChRβ2^2A-GAL4

48950

w¹¹¹⁸; P{GMR21F08-GAL4}attP2

49863

w¹¹¹⁸; P{GMR21F03-GAL4}attP2

68528

w¹¹¹⁸; P{R21F03-GAL4.DBD}attP2

84417

TI{2A-lexA::p65}nAChRβ2^2A-lexA

84545

w^*; TI{TI}nAChRβ2^attP

72341

w¹¹¹⁸; P{VT046831-p65.AD}attP40

73115

w¹¹¹⁸; P{VT046828-p65.AD}attP40

Korea

12775

P{VT046831-GAL4}attP2

VDRC

v109450

P{KK108779}VIE-260B

v204946

P{VT046828-GAL4}attP2

v1200

w¹¹¹⁸; P{GD237}v1200

More stocks available...

Genomic Clones (20)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (48)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH09870

Other clones

FI19416

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0004118

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0004118

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Amourda and Saunders, 2020, Chamaon et al., 2002)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Acr96Ac is expressed at high levels during late embryonic and late pupal stages, the transcripts are localized in the CNS.

(Sawruk et al., 1990)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: nAcRβ-96A CG6798

(Kodira, 1999.11)

Source for identity of: nAChRβ2 nAcRβ-96A

(FlyBase, 2009-)

Nomenclature comments

Renamed genes encoding nicotinic Acetylcholine Receptors to give systematic nomenclature that better reflects usage in literature (e.g. FBrf0218556, FBrf0183743).

(FlyBase, 2009-)

Etymology

Synonyms and Secondary IDs (25)

Reported As

Symbol Synonym

Acr96Ac

(Ranz et al., 1999)

AcrF

CG6798

(Mao et al., 2024, Kondo et al., 2020, Transgenic RNAi Project members, 2017-, Rieder et al., 2015, Tsubouchi et al., 2012, Keleman et al., 2009.8.5, Perkins et al., 2009, Bhutkar et al., 2008, Dent, 2006, Dallman et al., 2004)

Dmβ2

(Zhang et al., 2025)

Dβ2

(Komori et al., 2023, Nakayama et al., 2023, Perry et al., 2021, Ihara et al., 2020, Denecke et al., 2015, Wu et al., 2014, Dupuis et al., 2012, Jepson and Reenan, 2009, Perry et al., 2008, Dent, 2006)

EMS2

(Pyakurel et al., 2018, Shin et al., 2018)

SBD

(Stocker et al., 2013, Hoopengardner et al., 2003, Chamaon et al., 2002, Lansdell and Millar, 2002, Lansdell and Millar, 2000, Lansdell and Millar, 2000, Schulz et al., 1998, Lansdell et al., 1997, Schulz et al., 1997, Broughton et al., 1996, Tomizawa et al., 1996, Thomas et al., 1995, Nedlina-Chittka and Gundelfinger, 1993, Gundelfinger, 1992)

nACR beta 96A

(Zhan et al., 2013)

nACRbeta

(Zhan et al., 2013)

nAChR

(Fernandez-Chiappe et al., 2025, Barber et al., 2021, Rodrigues et al., 2021, Tomizawa et al., 2003, Wolf and Heberlein, 2003, Schafer, 2002)

nAChR-β96A

(Ren et al., 2012)

nAChRbeta2

(Deshpande et al., 2022, Kondo et al., 2020, Rao and Deng, 2019.10.23, Gene Disruption Project members, 2018-, Transgenic RNAi Project members, 2017-, Gene Disruption Project members, 2015-)

nAChRβ2

(Fujita et al., 2024, Mao et al., 2024, Korona et al., 2022, Lu et al., 2022, Jin et al., 2021, Rosenthal and Yuan, 2021, Tasman et al., 2021, Amourda and Saunders, 2020, Mazzotta et al., 2020, Meltzer et al., 2019, Shih et al., 2019, Croset et al., 2018, Pyakurel et al., 2018)

nAcRbeta-96A

(Crocker et al., 2016, Zhan et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Rodriguez et al., 2012, Bhutkar et al., 2008, Burgler and Macdonald, 2005)

nAcRs

(Ranz et al., 2001)

nAcRβ

(Dallman et al., 2004)

nAcRβ-96A

(Tsubouchi et al., 2012, Takemura et al., 2011)

nAcr96Ac

(Ranz et al., 2001)

sbd

(Rieder et al., 2015, Savva et al., 2012, Jepson et al., 2011, Ward et al., 2001, Millar, 1997.8.28, Pereira et al., 1997)

β2

(Liu et al., 2010)

β₂

(Tsunoyama and Gojobori, 1998)

Name Synonyms

Dβ2

(Sattelle et al., 2005)

SBD

(Sattelle et al., 2005)

nicotinic Acetylcholine Receptor beta 96A

(Tsubouchi et al., 2012)

nicotinic Acetylcholine Receptor β2

(Shafer, 2025)

nicotinic acetylcholine receptor β2

(Amourda and Saunders, 2020)

second beta-like subunit of Drosophila

(Chamaon et al., 2002, Gundelfinger, 1992)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 48 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

42920

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P25162

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P25162

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P25162

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42920/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0004118

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0004118

FlyMine - An integrated database for Drosophila genomics

FBgn0004118

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG6798

MARRVEL_MODEL - MARRVEL (model organism gene)

42920

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0004118

DroID - A comprehensive database of gene and protein interactions.

FBgn0004118

DRSC - Results frm RNAi screens

FBgn0004118

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

nAChRbeta2_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0004118

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0004118

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6798

Flygut - An atlas of the Drosophila adult midgut

FBgn0004118

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0004118

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (126)

The following external sites may use different assemblies or annotations than FlyBase.

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