FlyBase Gene Report: Dmel\nAChRα5

General Information

Symbol

Dmel\nAChRα5

Species

D. melanogaster

Name

nicotinic Acetylcholine Receptor α5

Annotation Symbol

CG32975

Feature Type

protein_coding_gene

FlyBase ID

FBgn0028875

Gene Model Status

Current

Stock Availability

110 publicly available

Gene Summary

nicotinic Acetylcholine Receptor α5 (nAChRα5) encodes a subunit of the nicotinic acetylcholine receptor, which is a pentameric neurotransmitter-gated ion channel. [Date last reviewed: 2018-10-11] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

Dα5, nAcRalpha-34E, BG:DS05899.4 , nAChR, nAcRα-34E

Key Links

Genomic Location

Cytogenetic map

34F2-34F4

Sequence location

2L:14,040,170..14,094,401 [+]

Recombination map

2-50

RefSeq locus

NT_033779 REGION:14040170..14094401

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (18 terms)

Molecular Function (6 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables acetylcholine binding

inferred from direct assay

(Wu et al., 2005)

contributes_to acetylcholine-gated monoatomic cation-selective channel activity

inferred from direct assay

(Watson et al., 2010)

enables acetylcholine-gated monoatomic cation-selective channel activity

inferred from direct assay

(Lansdell et al., 2012)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

contributes_to acetylcholine-gated monoatomic cation-selective channel activity

inferred from biological aspect of ancestor with PANTHER:PTN001054960

(GO Reference Genome Project, 2011-)

enables acetylcholine-gated monoatomic cation-selective channel activity

inferred from electronic annotation with InterPro:IPR002394

(InterPro Project Members, 2004-)

enables extracellular ligand-gated monoatomic ion channel activity

inferred from electronic annotation with InterPro:IPR006202, InterPro:IPR036734

(InterPro Project Members, 2004-)

enables monoatomic ion channel activity

inferred from electronic annotation with InterPro:IPR002394, InterPro:IPR006201

(InterPro Project Members, 2004-)

enables neurotransmitter receptor activity

inferred from biological aspect of ancestor with PANTHER:PTN000434994

(GO Reference Genome Project, 2011-)

enables transmembrane signaling receptor activity

inferred from electronic annotation with InterPro:IPR006201

(InterPro Project Members, 2004-)

Biological Process (6 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in monoatomic cation transport

inferred from direct assay

(Lansdell et al., 2012, Watson et al., 2010)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

involved_in calcium ion transport

inferred from biological aspect of ancestor with PANTHER:PTN000434994

(GO Reference Genome Project, 2011-)

involved_in monoatomic ion transmembrane transport

inferred from electronic annotation with InterPro:IPR006201

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000434994

(GO Reference Genome Project, 2011-)

involved_in monoatomic ion transport

inferred from electronic annotation with InterPro:IPR002394, InterPro:IPR006029, InterPro:IPR006202, InterPro:IPR036719, InterPro:IPR036734

(InterPro Project Members, 2004-)

inferred from electronic annotation with InterPro:IPR002394, InterPro:IPR006202, InterPro:IPR036734

(InterPro Project Members, 2004-)

involved_in regulation of membrane potential

inferred from biological aspect of ancestor with PANTHER:PTN000434994

(GO Reference Genome Project, 2011-)

involved_in synaptic transmission, cholinergic

inferred from biological aspect of ancestor with PANTHER:PTN001054960

(GO Reference Genome Project, 2011-)

Cellular Component (6 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

part_of acetylcholine-gated channel complex

inferred from physical interaction with FLYBASE:nAChRα6; FB:FBgn0032151,FLYBASE:nAChRα7; FB:FBgn0086778

(Lansdell et al., 2012)

inferred from direct assay

(Watson et al., 2010)

located_in membrane

inferred from direct assay

(Watson et al., 2010)

Terms Based on Predictions or Assertions (6 terms)

CV Term

Evidence

References

part_of acetylcholine-gated channel complex

inferred from biological aspect of ancestor with PANTHER:PTN001825278

(GO Reference Genome Project, 2011-)

located_in membrane

inferred from electronic annotation with InterPro:IPR002394, InterPro:IPR006029, InterPro:IPR006201, InterPro:IPR006202, InterPro:IPR036719, InterPro:IPR036734

(InterPro Project Members, 2004-)

inferred from electronic annotation with InterPro:IPR002394, InterPro:IPR006201, InterPro:IPR006202, InterPro:IPR036734

(InterPro Project Members, 2004-)

is_active_in neuron projection

inferred from biological aspect of ancestor with PANTHER:PTN000434994

(GO Reference Genome Project, 2011-)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN001054635

(GO Reference Genome Project, 2011-)

located_in postsynaptic membrane

inferred from electronic annotation with InterPro:IPR002394

(InterPro Project Members, 2004-)

is_active_in synapse

inferred from biological aspect of ancestor with PANTHER:PTN000434994

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

NICOTINIC ACETYLCHOLINE RECEPTOR SUBUNITS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

nicotinic Acetylcholine Receptor α5 (nAChRα5) encodes a subunit of the nicotinic acetylcholine receptor, which is a pentameric neurotransmitter-gated ion channel. [Date last reviewed: 2018-10-11]

Gene Group (FlyBase)

NICOTINIC ACETYLCHOLINE RECEPTOR SUBUNITS -: Nicotinic ligand-gated ion channels (nAChR) mediate the fast actions of the acetylcholine (ACh). The non-selective ion channel is formed from five subunits arranged around a central pore. The ACh binding site is formed by two adjacent α subunits. Other regulatory subunits may modulate channel pharmacology and expression. (Adapted from FBrf0183743).

Summary (Interactive Fly)

neurotransmitter-gated ion-channels - cholinergic synapse formation and dendritic refinement - site of action of insecticides

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\nAChRα5 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A0A499GGQ3)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.52

Gene model reviewed during 5.45

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.41

Gene model reviewed during 5.55

Stop-codon suppression (UAG) postulated; FBrf0234051.

Gene model reviewed during 6.25

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

nAChRα5-RB

FBtr0080594

NM_205986

4396

808

nAChRα5-RD

FBtr0300594

NM_205985

9129

808

nAChRα5-RH

FBtr0309867

NM_001259098

7755

808

nAChRα5-RI

FBtr0474155

NM_001370005

7755

837

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

nAChRα5-PB

FBpp0089367

91.3

808

7.30

FBpp0289821

91.3

808

7.30

FBpp0301601

91.3

808

7.30

FBpp0423154

94.8

837

7.87

AAF53374

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

808 aa isoforms: nAChRα5-PB, nAChRα5-PD, nAChRα5-PH

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\nAChRα5 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

nAChRalpha5_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.43

Transcript Expression

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

lamina monopolar neuron L4

(Takemura et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of acetylcholine-gated channel complex

inferred from physical interaction with FLYBASE:nAChRα6; FB:FBgn0032151,FLYBASE:nAChRα7; FB:FBgn0086778

(Lansdell et al., 2012)

inferred from direct assay

(Watson et al., 2010)

located_in membrane

inferred from direct assay

(Watson et al., 2010)

Expression Deduced from Reporters

Reporter: TI{2A-lexA::GAD}nAChRα5^2A-lexA

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

DN1p neuron | subset

(Jin et al., 2021)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\nAChRα5 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\nAChRα5

View exonic expression by tissue for Dmel\nAChRα5

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34826/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0028875

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0028875

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0028875

Flygut - An atlas of the Drosophila adult midgut

FBgn0028875

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 20 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 18 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of nAChRα5

Transgenic constructs containing regulatory region of nAChRα5

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(2L)Exel8032

(Ryder, 2004.4.26)

Inferred from location ( 6 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Dcr-2^UAS.cDa, Scer\GAL4^e22c, Scer\GAL4^sqh.PW

lethal, with Scer\GAL4^Mef2.PR

nAChRα5^GD1138

lethal, with Scer\GAL4^pnr-MD237

nAChRα5^GD1138

partially lethal - majority die, with Scer\GAL4^pnr-MD237

nAChRα5^GD15157

viable

nAChRα5^MI05549

viable, with Dcr-2^UAS.cDa, Scer\GAL4^e22c, Scer\GAL4^sqh.PW

nAChRα5^GD15691

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

viable, with Scer\GAL4^Mef2.PR

nAChRα5^GD15691

viable, with Scer\GAL4^pnr-MD237

nAChRα5^GD15691

viable, with Scer\GAL4^tin.CΔ4

nAChRα5^GD15691

Other Phenotypes

Allele

abnormal memory | adult stage, with Scer\GAL4^ple.PF

nAChRα5^JF01963

chemical resistant

nAChRα5^KO.dsRed+

some die during pupal stage, with Scer\GAL4^pnr-MD237

nAChRα5^GD15157

visible, with Scer\GAL4^pnr-MD237

nAChRα5^GD15157

visible | adult stage

nAChRα5^KO.dsRed+

Phenotype manifest in

Allele

adult abdomen

nAChRα5^KO.dsRed+

mesonotum, with Scer\GAL4^pnr-MD237

nAChRα5^GD15157

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (25)

Hsap\CHRNA7

12 of 14

Yes

11 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\CHRNG

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (18)

Rnor\Chrna7

11 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Htr3a

1 of 14

Rnor\Htr3b

1 of 14

Mus musculus (laboratory mouse) (18)

Mmus\Chrna7

12 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (14)

Xtro\chrna7

10 of 13

Yes

Xtro\lman2l

8 of 13

Xtro\LOC100487782

8 of 13

Xtro\chrna9

2 of 13

Yes

Xtro\chrna10

2 of 13

Yes

Xtro\adal

1 of 13

Xtro\chrna2

1 of 13

Xtro\chrne

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\LOC100494266

1 of 13

Xtro\LOC116410385

1 of 13

Yes

Danio rerio (Zebrafish) (34)

Drer\chrna7a

11 of 14

Yes

10 of 14

10 of 14

10 of 14

2 of 14

Yes

Drer\chrna10a

2 of 14

Drer\LOC798522

2 of 14

Yes

Drer\LOC101882637

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\LOC100334841

1 of 14

Drer\LOC101884593

1 of 14

Yes

Drer\LOC101885757

1 of 14

Yes

Drer\LOC103909988

1 of 14

Yes

Drer\LOC110439304

1 of 14

Drer\si:ch211-256e16.4

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (65)

Cele\acr-16

11 of 14

Yes

Cele\acr-11

9 of 14

Yes

Cele\acr-9

7 of 14

Yes

Cele\acr-25

7 of 14

Yes

Cele\acr-7

5 of 14

Yes

Cele\acr-10

5 of 14

Yes

Cele\acr-14

5 of 14

Yes

Cele\acr-15

4 of 14

Yes

Cele\acr-19

4 of 14

Cele\eat-2

4 of 14

Cele\acr-18

3 of 14

Yes

Cele\lgc-12

3 of 14

Cele\acr-6

2 of 14

Cele\acr-17

2 of 14

Yes

Cele\acr-21

2 of 14

Yes

Cele\lgc-1

2 of 14

Cele\lgc-4

2 of 14

Yes

Cele\lgc-5

2 of 14

Yes

Cele\lgc-6

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Cele\lgc-28

2 of 14

Yes

Cele\lgc-29

2 of 14

Cele\pbo-5

2 of 14

Yes

Cele\pbo-6

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\lgc-30

1 of 14

Cele\lgc-31

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (16)

Agam\AgaP_AGAP008588

11 of 12

Yes

5 of 12

4 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Agam\AgaP_AGAP012581

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:nAChRα5. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (22)

11 of 13

11 of 13

4 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

Grd

2 of 13

Lcch3

2 of 13

nAChRα2

2 of 13

NtR

2 of 13

pHCl-2

2 of 13

Rdl

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

ort

1 of 13

pHCl-1

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

CHRNA7; cholinergic receptor nicotinic alpha 7 subunit

12 of 14

118511

CHRFAM7A; CHRNA7 (exons 5-10) and FAM7A (exons A-E) fusion

11 of 14

609756

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

nAChRα5 - hig

anti tag coimmunoprecipitation, western blot

(Nakayama et al., 2023)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0028875

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

gene_with_edited_transcript

(Rodriguez et al., 2012)

gene_with_stop_codon_read_through

(Jungreis et al., 2016)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-50

Cytogenetic map

34F2-34F4

Sequence location

2L:14,040,170..14,094,401 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

34F2-34F4

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-50

(FlyBase, 2016)

2-45.6

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (110)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Korea

12052

P{VT007174-GAL4}attP2

12580

P{VT007166-GAL4}attP2

13372

P{VT007171-GAL4}attP2

13664

P{VT007170-GAL4}attP2

13665

P{VT007182-GAL4}attP2

15007

P{VT007178-GAL4}attP2

15434

P{VT007175-GAL4}attP2

15452

P{VT007177-GAL4}attP2

15978

P{GMR21D05-lexA}JK22C

15979

P{GMR21D05-lexA}JK73A

16878R-2

v101820

More stocks available...

Genomic Clones (52)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (10)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE50045

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0028875

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RE04043

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0028875

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Nakayama et al., 2023)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Source for merge of: nAcRα-34E anon-WO02059370.44

(FlyBase, 1996-)

Additional comments

Annotations CG16878, CG4498 merged as CG32975 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

nAcRα-34E is not DopR.

(Ashburner et al., 1999.10.12)

Source for identity of nAcRα-34E BG:DS05899.4 BG:DS05899.5 was sequence comparison ( date:020421 ).

(FlyBase, 1996-)

Source for merge of nAcRα-34E anon-WO02059370.44 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: BG:DS05899.4 CG4498

(Kodira, 1999.11)

Source for identity of: nAcRα-34E BG:DS05899.4 BG:DS05899.5

(FlyBase, 1996-)

Source for identity of: nAChRα5 nAcRα-34E

(FlyBase, 2009-)

Nomenclature comments

Renamed genes encoding nicotinic Acetylcholine Receptors to give systematic nomenclature that better reflects usage in literature (e.g. FBrf0218556, FBrf0183743).

(FlyBase, 2009-)

Etymology

Synonyms and Secondary IDs (24)

Reported As

Symbol Synonym

AChRα5

(Lyutova et al., 2019)

BG:DS05899.4

(Ashburner et al., 1999, Ashburner et al., 1999, Ashburner et al., 1999, Ashburner et al., 1999.10.12)

BG:DS05899.5

(Ashburner et al., 1999, Ashburner et al., 1999)

CG16878

CG32975

(Mao et al., 2024, National Institute of Genetics Fly Stocks, 2023-, National Institute of Genetics Fly Stocks, 2022-, Transgenic RNAi Project members, 2017-, Hosono et al., 2015, Rieder et al., 2015, Tsubouchi et al., 2012, Keleman et al., 2009.8.5, Perkins et al., 2009, Perkins et al., 2009.1.15, Agrawal and Stormo, 2005)

CG4498

CT14633

(Littleton and Ganetzky, 2000)

Dα5

(Nakayama et al., 2023, Shao et al., 2022, Ffrench-Constant et al., 2016, Nakayama et al., 2016, Perry et al., 2015, Wu et al., 2014, Dupuis et al., 2012, Lansdell et al., 2012, Jepson et al., 2011, Takemura et al., 2011, Watson et al., 2010, Jepson and Reenan, 2009, Wu et al., 2005, Hoopengardner et al., 2003, Grauso et al., 2002)

Nic 34E

(Keegan et al., 2011)

anon-WO02059370.44

da5

(Rieder et al., 2015)

daf

(Savva et al., 2012)

lincRNA.128

(FlyBase Genome Annotators, 2014, Young et al., 2012)

lincRNA.S1055

(FlyBase Genome Annotators, 2014, Young et al., 2012)

nAChR

(Barber et al., 2021, Rodrigues et al., 2021, Tomizawa et al., 2003, Wolf and Heberlein, 2003, Schafer, 2002)

nAChRalpha5

(Zhao et al., 2025, Deshpande et al., 2022, National Institute of Genetics Fly Stocks, 2022-, Hope et al., 2020, Rao and Deng, 2019.10.23, Gene Disruption Project members, 2016-, Jungreis et al., 2016, Gene Disruption Project members, 2015-)

nAChRα-34E

(Wolfram et al., 2014)

nAcRalpha-34E

(Lovejoy et al., 2021, Crocker et al., 2016, Japanese National Institute of Genetics, 2012.5.21, Rodriguez et al., 2012, Rodriguez et al., 2012)

nAcRalpha-34e

(Kern et al., 2015)

nAcRα-34E

(Tsubouchi et al., 2012, Takemura et al., 2011, Glazov et al., 2005)

Name Synonyms

Dα5

(Sattelle et al., 2005)

nicotinic Acetylcholine Receptor α 34E

(Tsubouchi et al., 2012)

nicotinic Acetylcholine Receptor α5

(Shafer, 2025)

nicotinic Acetylcholine Receptor_34E

(Glazov et al., 2005)

Secondary FlyBase IDs

FBgn0028874
FBgn0066016

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 52 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34826

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A0A499GGQ3

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A0A499GGQ3

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34826/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0028875

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0028875

FlyMine - An integrated database for Drosophila genomics

FBgn0028875

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG32975

MARRVEL_MODEL - MARRVEL (model organism gene)

34826

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0028875

DroID - A comprehensive database of gene and protein interactions.

FBgn0028875

DRSC - Results frm RNAi screens

FBgn0028875

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

nAChRalpha5_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0028875

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0028875

Flygut - An atlas of the Drosophila adult midgut

FBgn0028875

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0028875

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

References (134)

The following external sites may use different assemblies or annotations than FlyBase.

-0.43

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