[34E1-34E1];[34F3-34F3];
A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
34E1;34F3
Breakpoint based on release 3 sequence coordinate from Thibault et al., 2004, Supplementary Table 2 (FBrf0174227) or 3 (FBrf0174228), converted to release 5 coordinate.
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
The presence of P+PBac{XP5.WH5}BSC253 was verified using the PCR methods and primers described in FBrf0175003.
Exelixis, Inc. determined the insertion site of P{XP}d03477 to be at Release 3 genomic coordinate 13860811 on chromosome arm 2L. This corresponds to 34E1 on the Release 3 and 4 genome maps. The predicted position of PBac{WH}nAcRalpha-34Ef00872 on the Release 4 map is 34F3. Consequently, the cytological breakpoints of Df(2L)BSC253 are predicted to be 34E1;34F3.