FlyBase Gene Report: Dmel\Pburs

General Information

Symbol

Dmel\Pburs

Species

D. melanogaster

Name

Partner of Bursicon

Annotation Symbol

CG15284

Feature Type

protein_coding_gene

FlyBase ID

FBgn0264810

Gene Model Status

Current

Stock Availability

15 publicly available

Gene Summary

Partner of Bursicon (Pburs) encodes a cystine knot protein that dimerizes with the product of Burs to form the bursicon neurohormone that acts on the receptor encoded by rk. Bursicon neurohormone is released after adult emergence and is required for tanning and hardening of the cuticle and expansion of the wings. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Red Book
Interactive Fly

Also Known As

bursicon β, pu, bursβ, burs β, bursicon

Key Links

Genomic Location

Cytogenetic map

35B1-35B1

Sequence location

2L:14,416,729..14,417,331 [-]

Recombination map

2-50

RefSeq locus

NT_033779 REGION:14416729..14417331

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (9 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

enables G protein-coupled receptor binding

inferred from direct assay

(Luo et al., 2005)

contributes_to neuropeptide hormone activity

inferred from direct assay

(Luo et al., 2005)

contributes_to neuropeptide receptor binding

inferred from physical interaction with FLYBASE:rk; FB:FBgn0003255

(Luo et al., 2005)

enables protein heterodimerization activity

inferred from physical interaction with UniProtKB:Q9VD83

(Mendive et al., 2005)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables G protein-coupled receptor binding

inferred from electronic annotation with InterPro:IPR034441

(InterPro Project Members, 2004-)

contributes_to neuropeptide hormone activity

inferred by curator from GO:0031395

(Mendive et al., 2005)

enables neuropeptide hormone activity

inferred from electronic annotation with InterPro:IPR034441

(InterPro Project Members, 2004-)

Biological Process (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in chitin-based cuticle sclerotization

inferred from mutant phenotype

(Luo et al., 2005)

involved_in ecdysis, chitin-based cuticle

inferred from mutant phenotype

(Lahr et al., 2012)

involved_in G protein-coupled receptor signaling pathway

inferred from direct assay

(Luo et al., 2005)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in G protein-coupled receptor signaling pathway

inferred from biological aspect of ancestor with PANTHER:PTN002221985

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

part_of bursicon neuropeptide hormone complex

inferred from physical interaction with FLYBASE:Burs; FB:FBgn0038901

(Luo et al., 2005)

inferred from direct assay

(Mendive et al., 2005)

located_in extracellular region

inferred from direct assay

(Luo et al., 2005)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

part_of bursicon neuropeptide hormone complex

inferred from electronic annotation with InterPro:IPR034441

(InterPro Project Members, 2004-)

is_active_in extracellular region

inferred from biological aspect of ancestor with PANTHER:PTN002221985

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

NEUROPEPTIDES

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

NEUROPEPTIDES -: Neuropeptides are secreted into the extracellular space where they interact with cell surface receptors (usually G protein coupled receptors). They are extremely diverse, acting as neurotransmitters, neuromodulators, hormones or growth factors. (Adapted from FBrf0211443 and PMID:27813667).

Protein Function (UniProtKB)

Final heterodimeric neurohormone released at the end of the molting cycle, involved in the sclerotization (tanning) of the insect cuticle, melanization and wing spreading. Heterodimer specifically activates the G protein-coupled receptor rk.

(UniProt, Q9VJS7)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

pu: pupal

Wings unexpanded or incompletely expanded; more extreme at 19. Extreme alleles show erect and crossed postscutellar bristles and a distorted thoracic cage (Ashburner). Df(2L)A400/Df(2L)el4DA80P, a homozygous pu deficiency, is barely viable; escapers are pu.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Pburs for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VJS7)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.51

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Pburs-RA

FBtr0080634

NM_135868

545

141

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Pburs-PA

FBpp0080207

16.0

141

5.28

Q9VJS7

NP_609712

AAF53396

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Heterodimer with Burs; 2 cystine knot polypeptides.

(UniProt, Q9VJS7)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9VJS7

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Pburs using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Pburs_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.21

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

neuron | subset of larval thoracic segment | restricted

(Luo et al., 2005)

neuron | subset of larval abdominal segment | restricted

(Luo et al., 2005)

pharate adult stage P8 -- pharate adult stage P15(ii)

neuron | subset of larval ventral nerve cord

(Lahr et al., 2012)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

early third instar larval stage -- adult stage

organism

(Luo et al., 2005)

Additional Descriptive Data

burs transcript is detected in 4 pairs of neurons in the pharate pupae ventral nerve cord, in segments A1 to A4. Strong expression is detected in one neuron in each T3 and A6 segments and 2 neurons in segments A1 to A5. All of these neurons also express CCAP transcript and burs protein and transcript.

(Lahr et al., 2012)

RT-PCR analysis indicates that pburs transcript levels are low in the third larval stage, gradually increasing during pupal stages and reaching the highest levels in pharate adults. Levels are low in adults. pburs transcript is also detected in four pairs of bilateral neurons in the thoracic and abdominal segments of a third instar larva.

(Luo et al., 2005)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

neuron | subset of abdominal neuromere

(Peabody et al., 2008)

neuron | subset of larval thoracic segment | restricted

(Luo et al., 2005)

neuron | subset of larval abdominal segment | restricted

(Luo et al., 2005)

Additional Descriptive Data

In the larval ventral cord, pburs is prominently expressed in a limited a number of neurons of the first four abdominal segments (A1-A4).

(Peabody et al., 2008)

pburs protein is detected in four pairs of bilateral neurons in the thoracic and abdominal segments of a third instar larva. These neurons also co-express burs protein.

(Luo et al., 2005)

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of bursicon neuropeptide hormone complex

inferred from physical interaction with FLYBASE:Burs; FB:FBgn0038901

(Luo et al., 2005)

inferred from direct assay

(Mendive et al., 2005)

located_in extracellular region

inferred from direct assay

(Luo et al., 2005)

Expression Deduced from Reporters

Reporter: P{Pburs-GAL4.P}

Stage

Tissue/Position (including subcellular localization)

Reference

prepupal stage

larval A1-A4 CCAP neuron

(Kim et al., 2015)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Pburs in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Pburs

View exonic expression by tissue for Dmel\Pburs

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34845/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0264810

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0032546

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0264810

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 17 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 11 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Pburs

Transgenic constructs containing regulatory region of Pburs

Aberrations (Deficiencies and Duplications) ( 157 )

Inferred from experimentation ( 157 )

Gene disrupted in

Df(2L)64j

(Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)A47

(Roote et al., 1996, Gubb et al., 1990, Ashburner et al., 1982)

Df(2L)A215

(Ashburner et al., 1982)

Df(2L)A217

(Ashburner et al., 1982)

Df(2L)A246

(Ashburner et al., 1982)

Df(2L)A263

(Ashburner et al., 1982)

Df(2L)A376

(Ashburner et al., 1982)

Df(2L)A377

(Ashburner et al., 1982)

Df(2L)A400

(Davis et al., 1997, Roote et al., 1996, Davis et al., 1990, Ashburner et al., 1982, Ashburner et al., 1982)

Df(2L)b81a1

(Roote et al., 1996, Ashburner et al., 1982)

Df(2L)b81a2^LA80^R

(Roote, 2000.7.3, Iyengar et al., 1999)

Df(2L)b83d29a

(Roote, 2000.7.3, Davis et al., 1997, Roote et al., 1996, Davis et al., 1990)

Df(2L)b84a2

(Davis et al., 1997, Roote et al., 1996, Davis et al., 1990)

Df(2L)b84a4

(Roote et al., 1996)

Df(2L)el77

(Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

Df(2L)el80f1

(Ashburner et al., 1983)

Df(2L)el94

(Roote, 1996.1.11, Roote, 1996.8, Roote, 1996.9.18)

(Roote, 2000.6.13)

(Ryder, 2004.4.26)

(Ryder, 2004.4.26)

(Grau et al., 1984, Ashburner et al., 1982, Ashburner et al., 1982, Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)fn5

(Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)fn7

(Ashburner et al., 1982, Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)fn12

(Ashburner et al., 1982, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)fn26

(Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)fn30

(Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)fn31

(Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)fn36

(Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)GT4

(Gubb et al., 1990)

Df(2L)GT5

(Gubb et al., 1990)

Df(2L)Sco7

(Ashburner et al., 1983)

Df(2L)Sco^rv23

(Grau et al., 1984, Ashburner et al., 1983)

Df(2L)TE35B-2

(Gubb et al., 1985)

Df(2L)TE35B-3

(Gubb et al., 1985)

Df(2L)TE35B-4

(Gubb et al., 1985)

Df(2L)TE35B-5

(Gubb et al., 1985)

Df(2L)TE35B-7

(Davis et al., 1997, Roote et al., 1996, Davis et al., 1990, Gubb et al., 1985)

Df(2L)TE35B-8

(Roote et al., 1996, Gubb et al., 1985)

Df(2L)TE35B-9

(Gubb et al., 1985)

Df(2L)TE35B-10

(Gubb et al., 1985)

Df(2L)TE35B-12

(Roote, 1996.8, Roote et al., 1996, Gubb et al., 1985)

Df(2L)TE35B-14

(Roote, 1996.8)

Df(2L)TE35B-15

(Gubb et al., 1985)

Df(2L)TE35B-300

(Roote et al., 1996)

Df(2L)TE35BC-6

(Gubb et al., 1984)

Df(2L)TE35BC-23

(Gubb et al., 1984)

Df(2L)TE35BC-29

(Gubb et al., 1984)

Df(2L)TE35BC-31

(Gubb et al., 1984)

Df(2L)W

(Ashburner et al., 1982, Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

In(2L)75c^LC158.1^R

(Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

In(2L)75c

(Grau et al., 1984, Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

In(2L)el⁹

(Davis et al., 1990)

In(2L)TE35B-13

(Gubb et al., 1985)

In(2LR)D20^Lnoc^4R

(Roote et al., 1996)

In(2LR)el^6LA379^R

(Davis, 2001.4.23, Roote et al., 1996, Roote, 1995.3.27)

T(2;3)dpp^s19

(Davis et al., 1990)

T(Y;2)GT2

(Gubb et al., 1990)

Tp(2;3)GT3

(Gubb et al., 1990)

Ts(2Lt;3Lt)dpp^s19+Ts(2Rt;3Rt)el24

(Roote et al., 1996)

Ts(2Lt;3Rt)GT10+Ts(2Rt;3Lt)TE35B-209

(Roote et al., 1996)

Ts(Y;2Lt)a15+Ts(YSt;2Rt)A80

(Davis et al., 1997, Roote, 1996.1.11, Roote, 1996.8, Roote et al., 1996, Roote, 1995.3.27)

Ts(Y;2Lt)el⁴+Ts(YSt;2Rt)A80

(Roote, 1996.1.11, Roote, 1996.8, Roote et al., 1996)

Ts(Y;2Lt)el⁴+Ts(YSt;2Rt)R15

(Davis et al., 1997, Roote et al., 1996, Ashburner et al., 1982, Ashburner et al., 1981)

Ts(Y;2Lt)GT2+Ts(YSt;2Rt)A80

(Davis et al., 1997, Roote, 1996.1.11, Roote, 1996.8, Roote et al., 1996)

Ts(YLt;2Lt)G74+Ts(YSt;2Rt)B210

(Woodruff and Ashburner, 1979)

Ts(YSt;2Lt)G74+Ts(YSt;2Rt)A80

(Roote et al., 1996, Roote, 1995.3.27, Woodruff and Ashburner, 1979)

Ts(YSt;2Lt)G74+Ts(YSt;2Rt)J165

(Woodruff and Ashburner, 1979)

Ts(YSt;2Lt)G74+Ts(YSt;2Rt)P58

(Woodruff and Ashburner, 1979)

Ts(YSt;2Lt)G74+Ts(YSt;2Rt)R15

(Woodruff and Ashburner, 1979)

Gene not disrupted in

Df(2L)A48

(Roote et al., 1996, Gubb et al., 1990, Grau et al., 1984, Ashburner et al., 1982, Ashburner et al., 1982)

Df(2L)A63

(Roote et al., 1996, Ashburner et al., 1982, Ashburner et al., 1982)

Df(2L)A72

(Ashburner et al., 1982, Ashburner et al., 1982)

Df(2L)A178

(Davis et al., 1997, Roote et al., 1996, Ashburner et al., 1982, Ashburner et al., 1982)

Df(2L)A220

(Ashburner et al., 1982)

Df(2L)A245

(Roote et al., 1996, Davis et al., 1990, Ashburner et al., 1982)

Df(2L)A260

(Davis et al., 1990, Ashburner et al., 1982)

Df(2L)A264

(Ashburner et al., 1982)

Df(2L)A266

(Davis et al., 1990, Ashburner et al., 1982, Ashburner et al., 1982)

Df(2L)A267

(Roote et al., 1996, Ashburner et al., 1982, Ashburner et al., 1982)

Df(2L)A379

(Ashburner et al., 1982, Ashburner et al., 1982)

Df(2L)A445

(Roote et al., 1996, Ashburner et al., 1982)

Df(2L)Adh-nBR102

(Roote et al., 1996)

Df(2L)ARR1

(Roote, 1996.8, Roote et al., 1996, Davis et al., 1990)

Df(2L)b75

(Gubb et al., 1990, Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

Df(2L)b77c

(Roote, 1995.3.27)

Df(2L)b79b8

(Roote, 1995.3.27)

Df(2L)b80e3

(Roote et al., 1996, Ashburner et al., 1982, Ashburner et al., 1981)

Df(2L)b88h49

(Roote, 1996.8, Roote et al., 1996)

Df(2L)b-L

(Ashburner et al., 1981)

Df(2L)do1

(Ashburner et al., 1981)

Df(2L)el14

(Davis, 2001.4.23, Roote et al., 1996, Davis et al., 1990)

Df(2L)el16

(Davis, 2001.4.23, Davis et al., 1997, Davis et al., 1990)

Df(2L)el28

(Davis et al., 1997, Davis et al., 1990)

Df(2L)EP(2)2039-rv4

(Roote, 2000.6.13)

Df(2L)fn2

(Davis et al., 1997, Roote et al., 1996, Grau et al., 1984, Ashburner et al., 1982, Ashburner et al., 1982, Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)fn3

(Davis et al., 1997, Davis et al., 1990, Ashburner et al., 1982, Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)fn15

(Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)fn27

(Davis et al., 1997, Ashburner et al., 1982, Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)n78l3

(Davis et al., 1997, Ashburner et al., 1982)

Df(2L)osp29

(Roote et al., 1996, Grau et al., 1984, Ashburner et al., 1982)

Df(2L)osp144

(Roote et al., 1996, Ashburner et al., 1982)

Df(2L)PA4

(Ashburner et al., 1983)

Df(2L)Sco^rv4

(Ashburner et al., 1990, Ashburner et al., 1983, Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

Df(2L)Sco^rv10

(Grau et al., 1984, Ashburner et al., 1983, Woodruff and Ashburner, 1979)

Df(2L)Sco^rv14

(Ashburner et al., 1983, Woodruff and Ashburner, 1979)

Df(2L)Sco^rv16

(Grau et al., 1984, Ashburner et al., 1983)

Df(2L)Sco^rv18

(Ashburner et al., 1983)

Df(2L)Sco^rv19

(Grau et al., 1984, Ashburner et al., 1983)

Df(2L)Sco^rv25

(Grau et al., 1984, Ashburner et al., 1983)

Df(2L)SS1

(Grau et al., 1984)

Df(2L)TE35B-1

(Davis et al., 1997, Davis et al., 1990, Gubb et al., 1985)

Df(2L)TE35B-6

(Davis et al., 1997, Davis et al., 1990, Gubb et al., 1985)

Df(2L)TE35B-11

(Roote et al., 1996, Gubb et al., 1985)

Df(2L)TE35B-54^L

(Roote et al., 1996)

Df(2L)TE35BC-1

(Gubb et al., 1984)

Df(2L)TE35BC-3

(Grau et al., 1984, Gubb et al., 1984, Ashburner et al., 1982)

Df(2L)TE35BC-4

(Roote et al., 1996, Gubb et al., 1984, Ashburner et al., 1982)

Df(2L)TE35BC-7

(Gubb et al., 1984)

Df(2L)TE35BC-8

(Davis et al., 1997, Davis et al., 1990, Gubb et al., 1984)

Df(2L)TE35BC-24

(Gubb et al., 1984)

Df(2L)TE35BC-28

(Gubb et al., 1984)

Df(2L)TE35BC-34

(Gubb et al., 1984)

Df(2L)TE35BC-35

(Gubb et al., 1984)

Dp(2;2)Cam12

(Roote et al., 1996)

In(2L)C158^LSco^rv11R

(Ashburner and Harrington, 1984, Ashburner et al., 1983)

In(2L)C158^LSco^rv17R

(Ashburner and Harrington, 1984, Ashburner et al., 1983)

In(2LR)DTD128

(Roote et al., 1996, Davis et al., 1990)

In(2LR)el⁶

(Davis et al., 1990)

In(2LR)noc^4LSco^rv9R

(Ashburner and Harrington, 1984)

In(2LR)TE35B-6^LSco^rv9R

(Ashburner and Harrington, 1984)

T(2;3)el24

(Davis et al., 1990)

T(2;3)GT8

(Davis et al., 1990)

T(2;3)GT10

(Davis et al., 1990)

T(2;3)Sco^rv7

(Ashburner and Harrington, 1984, Grau et al., 1984, Ashburner et al., 1983, Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

T(Y;2)a15

(Davis et al., 1990)

T(Y;2)A80^DR15^P

(Ashburner et al., 1982, Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

T(Y;2)A80

(Roote et al., 1996, Davis et al., 1990)

T(Y;2)el⁴

(Davis et al., 1990)

T(Y;2)GT2

(Davis et al., 1990)

T(Y;2)J165^DP58^P

(Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

T(Y;2)R15^DJ165^P

(Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

Tp(2;2)A446

(Davis et al., 1997, Roote et al., 1996, Ashburner et al., 1982, Ashburner et al., 1982)

Tp(2;3)GT3

(Davis et al., 1990)

Ts(2Lt;3Lt)TE35B-4+Ts(2Rt;3Rt)Sco^rv13

(Ashburner and Harrington, 1984)

Ts(2Lt;3Lt)TE35B-28+Ts(2Rt;3Rt)osp⁹⁰

(Roote et al., 1996)

Ts(2Lt;3Lt)TE35BC-3+Ts(2Rt;3Rt)Sco^rv13

(Ashburner and Harrington, 1984, Ashburner et al., 1983)

Ts(Y;2Lt)TE35B-18+Ts(YSt;2Rt)R15

(Davis et al., 1990)

Gene duplicated in

Dp(2;1)Sco^rv23

(Grau et al., 1984, Ashburner et al., 1983)

Ts(2Lt;4Lt)TE35B-101+Ts(2Rt;4Rt)GT6

(Roote et al., 1996)

Ts(YLt;2Lt)A80+Ts(Y;2Rt)el⁴

(Roote et al., 1996)

Ts(YLt;2Lt)A80+Ts(YLt;2Rt)G74

(Roote et al., 1996)

Ts(YLt;2Lt)R15+Ts(Y;2Rt)el⁴

(Roote et al., 1996)

Gene not duplicated in

Dp(2;2)TE35B-54^L

(Roote et al., 1996)

Dp(2;3)osp³

(Roote et al., 1996)

In(2L)C163.41^LC158^R

(Roote et al., 1996)

In(2LR)TE35B-226^LDTD128^R

(Roote et al., 1996)

Ts(Y;2Lt)TE35B-18+Ts(YSt;2Rt)A80

(Roote et al., 1996)

Ts(YLt;2Lt)R15+Ts(Y;2Rt)TE35B-18

(Roote et al., 1996)

Ts(YLt;2Lt)R15+Ts(YSt;2Rt)A80

(Roote et al., 1996)

Inferred from location ( 6 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | recessive

Pburs⁷

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Pburs^GD6814

viable, with Scer\GAL4^Mef2.PR

Pburs^GD6814

viable, with Scer\GAL4^pnr-MD237

Pburs^GD6814

Other Phenotypes

Allele

abnormal mating behavior | mated female, with Scer\GAL4^nSyb.PU

Pburs^GD6814

abnormal sleep | adult stage

Pburs^attP

visible

Pburs^unspecified

Phenotype manifest in

Allele

adult head

Pburs^exc

adult head (with Pburs^exc)

Pburs^pupal1

adult head (with Pburs^pupal1)

Pburs^exc

leg

Pburs^exc

leg (with Pburs^exc)

Pburs^pupal1

leg (with Pburs^pupal1)

Pburs^exc

pharate adult

Pburs^exc

pharate adult (with Pburs^exc)

Pburs^pupal1

pharate adult (with Pburs^pupal1)

Pburs^exc

pupal cuticle

Pburs^exc

pupal cuticle (with Pburs^exc)

Pburs^pupal1

pupal cuticle (with Pburs^pupal1)

Pburs^exc

wing

wing (with Pburs⁵)

Pburs^pupal1

wing (with Pburs^exc)

Pburs^pupal1

wing (with Pburs^pupal1)

wing hair | increased number, with Scer\GAL4^ap-md544

Pburs^GD6814

wing hair | increased number, with Scer\GAL4^dpp.blk1

Pburs^GD6814

wing hair | increased number, with Scer\GAL4^en-e16E

Pburs^GD6814

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (0)

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (0)

Mus musculus (laboratory mouse) (0)

Xenopus tropicalis (Western clawed frog) (0)

Danio rerio (Zebrafish) (0)

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (1)

Agam\PBURS_ANOGA

11 of 12

Yes

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Pburs. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (0)

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Pburs

comigration in non denaturing gel electrophoresis, western blot, confirmation by molecular weight

(An et al., 2012)

Pburs - Burs

molecular sieving, molecular weight estimation by staining, comigration in non denaturing gel electrophoresis, western blot, confirmation by molecular weight

(An et al., 2012, Mendive et al., 2005)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

wit

suppressible

CCAP|Pburs

(Veverytsa and Allan, 2011)

wit

suppressible

Pburs

(Veverytsa and Allan, 2011)

wit

suppressible

Mip|Pburs

(Veverytsa and Allan, 2011)

wit

suppressible

CCAP|Mip|Pburs

(Veverytsa and Allan, 2011)

External Data

Subunit Structure (UniProtKB)

Heterodimer with Burs; 2 cystine knot polypeptides.

(UniProt, Q9VJS7 )

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0264810

MIST (protein-protein) - An integrated Molecular Interaction Database

34845

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-50

Cytogenetic map

35B1-35B1

Sequence location

2L:14,416,729..14,417,331 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

35B1-35B1

Limits computationally determined from genome sequence between P{EP}elB^EP2039&P{PZ}osp^rJ571 and P{lacW}Su(H)^k07904

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-50

(FlyBase, 2016)

2-46.8

(Comeron et al., 2012)

2-[50]

Left of (cM)

Adh
PolD3

(Woodruff and Ashburner, 1979)

Curl

(Woodruff and Ashburner, 1979)

Right of (cM)

Adc
l(2)34Fc

(Woodruff and Ashburner, 1979)

Notes

Stocks and Reagents

Stocks (15)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

84682

TI{2A-GAL4}Pburs^2A-GAL4

84428

TI{2A-lexA::GAD}Pburs^2A-lexA/CyO

84560

w^*; TI{TI}Pburs^attP/CyO

80815

y¹, v¹; P{TKO.GS04621}attP40

56453

y¹ w^*; Mi{MIC}Pburs^MI11717/SM6a

25161

Pburs⁴ Adhⁿ⁷ cn¹ vg¹/CyO

Kyoto

101309

Adc^b-1 Pburs⁶ Adhⁿ⁴ / CyO

NIG-Fly

15284R-1

P{NIG.15284R}1

M2L-2503

y¹ w¹¹¹⁸; Pburs^SK2 P{neoFRT}40A/CyO-Tb

M2L-2504

y¹ w¹¹¹⁸; Pburs^SK3 P{neoFRT}40A/CyO-Tb

VDRC

v102690

P{KK112186}VIE-260B

v27142

w¹¹¹⁸; P{GD6814}v27142

More stocks available...

Genomic Clones (18)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (1)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

RT06915

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0264810

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Luo et al., 2005)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Bioactive D.melanogaster bursicon is a heterodimer composed of the burs and pburs polypeptides. burs protein by itself does not have bursicon bioactivity.

(Mendive et al., 2005)

Wings unexpanded or incompletely expanded; more extreme at 19^oC. Extreme alleles show erect and crossed postscutellar bristles and a distorted thoracic cage (Ashburner). Df(2L)A400/Ts(Y;2Lt)el⁴+Ts(YSt;2Rt)A80, a homozygous pu deficiency, is barely viable; escapers are pu.

Relationship to Other Genes

Source for database merge of

Additional comments

"pu" may correspond to "CG15284".

(Davis, 2003)

"CG15284" may correspond to "pu".

(Davis, 2001.4.23)

" BG:DS06238.4 " is probably "pu".

(Ashburner et al., 1999)

" BG:DS06238.4 " probably corresponds to "pu".

(Ashburner et al., 1999.10.12)

Not allelic to pads.

Nomenclature History

Source for database identify of

Source for identity of: pburs CG15284

(Luo et al., 2005)

Source for identity of: Pburs pburs

(FlyBase, 2009-)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (28)

Reported As

Symbol Synonym

Bur β

(Harwood et al., 2013)

Burs β

(Nässel and Winther, 2010)

Bursβ

(Veverytsa and Allan, 2011, McNabb and Truman, 2006)

CG15284

(Mao et al., 2024, National Institute of Genetics Fly Stocks, 2022-, Lahr et al., 2012, Weber et al., 2012, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, An et al., 2008, Natzle et al., 2008, Luan et al., 2006, McNabb and Truman, 2006, Mendive et al., 2005, Davis, 2003, Giot et al., 2003, Davis, 2001.4.23, Davis, 2001.10.11)

PBURS

(Nässel and Winther, 2010)

Pburs

(Kubrak et al., 2024, Garaulet et al., 2021, Lee et al., 2021, Rao and Deng, 2019.10.23, Transgenic RNAi Project members, 2017-, Chen et al., 2016, Lee et al., 2015, Hwang et al., 2014)

burs β

(An et al., 2012, Hari et al., 2008)

burs-beta

(VandenBroeck, 2004.11.9)

burs-β

(Scopelliti et al., 2016)

bursβ

(Lahr et al., 2012, Luan et al., 2006, Mendive et al., 2005)

pads-b

pburs

(Anllo and Schüpbach, 2016, Mena et al., 2016, Diao et al., 2015, Japanese National Institute of Genetics, 2012.5.21, Lahr et al., 2012, Luan et al., 2012, Weber et al., 2012, Gatto and Broadie, 2011, Kim and Marqués, 2010, Nässel and Winther, 2010, Zhong et al., 2010, Hari et al., 2008, Peabody et al., 2008, Engström et al., 2007, Bowser and Tobe, 2006, Luo et al., 2005)

(Kovalick, 2008, Natzle et al., 2008, Davis, 2003)

pup

(Ainsley et al., 2003)

Name Synonyms

Bursicon β subunit

(Nässel and Winther, 2010)

Partner of Bursicon

(Hwang et al., 2014)

Partner of burs

(Caers et al., 2012)

Partner of bursicon

(Nässel and Winther, 2010)

bursicon

(Kim et al., 2006, Kim et al., 2006)

bursicon β

(An et al., 2012, Gatto and Broadie, 2011, An et al., 2008, Mendive et al., 2005)

bursicon β subunit

(Gatto and Broadie, 2011)

bursicon β-subunit

(Peabody et al., 2008)

lethal group A

partner of burs

partner of bursicon

(Christesen et al., 2021, Kim and Marqués, 2010)

partner-of-bursicon

(Gatto and Broadie, 2011)

pupal

Secondary FlyBase IDs

FBgn0003161
FBgn0032546

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 23 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34845

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

AJ862524

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VJS7

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VJS7

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VJS7

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34845/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0264810

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0264810

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG15284

MARRVEL_MODEL - MARRVEL (model organism gene)

34845

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0264810

DroID - A comprehensive database of gene and protein interactions.

FBgn0264810

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Pburs_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0032546

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0264810

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC013831

MIST (protein-protein) - An integrated Molecular Interaction Database

34845

References (105)

The following external sites may use different assemblies or annotations than FlyBase.

-0.21

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