Subject: new P derivatives
Dear Rachel,
Here is a list of deletions that we have made over the last few years
by P-element excision and male recombination - some very informative,
some less so. All induced with Δ2-3.
Fondest regards,
Rootles
(pers comm that if you dare)
k07245  BG:DS00797.1 
k07245-rv1 kuz-, l(2)34Db+.
rN149  BG:DS08220.1 
rn149-rv3 RpII33+, Ance-, j-, rk-, l(2)34Fa-, wb-,
ms(2)34Fe+
k11509 smi35A
k11509-rv18 wb-
k08712
k08712-rv2 l(2)34Fd-, semi-lethal, short bristles
k08712-rv21 l(2)35Aa-, lethal, rescued by 35Aa 5kb rescue fragment
k08712-rv34 l(2)34Fd-, lethal
EP(2)2039 elB
EP(2)2039-rv4 wb+, ms(2)34Fe-,l(2)34Fc-, l(2)34Fd-,
l(2)35Aa-, elB-, pu+
EP(2)2039-rv3 elB-, pu-
k13218 osp
225 w- revertants 7 were osp-, 3 were noc+/- osp-
k11524 l(2)35Bb
2 out of 7 excisions revert the lethality.
k08808 l(2)35Bc
121 excisions, 37 lethal over Df(2L)nBR107 (i.e. 84 precise
excisions): 31 imprecise excisions l(2)35Bc-, 5 deletions:
k08808-rv25 l(2)35Be+, l(2)35Bc-, l(2)35Bd-, l(2)35Bg+
k08808-rv52 l(2)35Be+, l(2)35Bc-, l(2)35Bd-, l(2)35Bg+
k08808-rv57 l(2)35Be+, l(2)35Bc-, l(2)35Bd-, l(2)35Bg+
k08808-rv63 l(2)35Be+, l(2)35Bc-, l(2)35Bd-, l(2)35Bg+
k08808-rv70 l(2)35Bf+, l(2)35Be-, l(2)35Bc-, l(2)35Bd+
Therefore the gene order is 35Bb, 35Bf, 35Be, 35Bc, 35Bd.
PZ05271 l(2)35Ea
1 excision out of 14 (from Julie Gates, Utah) is lethal.
PZ05271-rv54 l(2)35Ea-
k09834 PRL-1
k09834rv1 Ca-a1+, twe-, crp-, l(2)35Fb+
k09834rv2 Ca-a1+, twe-, crp+
k17027 cact
k17027rv1 fzy+, cact- (fs over H60-3), l(2)35Fe+
k17027rv2 cact+, l(2)35Fe-, chif+
k17027rv3 fzy+, cact- (fs over cactAR51.4), l(2)35Fe+
k04216 chif
RN2 chifWD18
k04216-rv1 female sterile female fertile!
k04216-rv2 female sterile female sterile
k04216-rv3 female sterile female sterile
k08106 multiple insert in 35F
14 excisions, selected as w-: 10 lethal, 4 semi-lethal and fs over
RN2, 6 retain weak w+.
k08106-rv4 cni+, fzy-, cact-, l(2)35Fe-, chif-,
l(2)35Fg-, dac+; w+ (weak)
k08106-rv6 cni+, fzy-, cact-, l(2)35Fe-, chif-,
l(2)35Fg-, dac+; w-
k08106-rv15 cni+, fzy-, cact-, l(2)35Fe-, chif-,
l(2)35Fg-, dac+; w-
Male recombinants - induced over dpov1 b1 cn1 bw1
k07245  BG:DS00797.1 
k07245-mr2 b kuz+, l(2)34Db- - Ance-.
k07245-mr9 dp kuz+/-
k07245-mr11 b kuz+, l(2)34Db- - wb-
k07245-mr28 dp kuz+/-, l(2)34Db+
k07245-mr31 b kuz+, l(2)34Db- - ms(2)35Ci-, esg+
k07245-mr32 b kuz+, l(2)34Db- - gft-, rd+
k05007 CycE
k05007-rv1 cn bw CycE+, l(2)35Di-, l(2)35Df+
mr1 cn bw lace+, CycE-, l(2)35Di-, l(2)35Df+
mr2 cn bw lace+, CycE-, l(2)35Di-,
l(2)35Df-, Gli-, l(2)35Ea+
mr3 cn bw lace+, CycE-, l(2)35Di-,
l(2)35Df-, Gli-, l(2)35Ea+
mr6 cn bw lace+, CycE-, l(2)35Di-,
l(2)35Df-, Gli-, l(2)35Ea+
mr9 cn bw lace+, CycE-, l(2)35Di-,
l(2)35Df-, Gli-, l(2)35Ea+
mr13 cn bw lace+, CycE-, l(2)35Di-,
l(2)35Df-, Gli-, l(2)35Ea+
mr15 cn bw lace+, CycE-, l(2)35Di-,
l(2)35Df-, Gli-, l(2)35Ea-, l(2)35Dh-, l(2)35De-
mr19 dp b vasa+, stc- - CycE-, l(2)35Di+
PZ07130 ck
PZ07130-mr1 dp b kuz- - ck-, l(2)35Cf+
PZ07130-mr2 cn bw Su(H)+, ck- - vasa-, stc+
PZ07130-mr3 cn bw Su(H)+, ck-, l(2)35Cf+
PZ07130-mr5 cn bw Su(H)+, ck- - esg-, wor+
PZ07130-mr7 cn bw Su(H)+, ck- - vasa-, stc+
PZ07130-mr8 cn bw Su(H)+, ck-, l(2)35Cf+
PZ07130-mr9 dp b l(2)35Bg- - ck-, l(2)35Cf+
PZ06430 gft
PZ06430-mr1 cn bw l(2)35Cc+, gft-, ms(2)35Ci+
mr2 dp b l(2)35Cc+, gft-, ms(2)35Ci+
mr3 cn bw l(2)35Cc+, gft-, ms(2)35Ci-, esg+
mr4 cn bw l(2)35Cc+, gft-, ms(2)35Ci-, esg+
mr5 cn bw l(2)35Cc+, gft-, ms(2)35Ci-, esg+
mr7 cn bw l(2)35Cc+, gft-, ms(2)35Ci-, esg+
mr8 cn bw l(2)35Cc+, gft-, ms(2)35Ci+
mr9 cn bw l(2)35Cc+, gft-, ms(2)35Ci-, esg+
mr10 cn bw l(2)35Cc+, gft-, ms(2)35Ci-, esg+
mr11 dp b l(2)35Cc+, gft-, ms(2)35Ci+
mr12 cn bw l(2)35Cc+, gft-, ms(2)35Ci-, esg+
mr13 cn bw l(2)35Cc+, gft-, ms(2)35Ci+
mr14 dp b rd+, l(2)35Cc, gft-, ms(2)35Ci+
PZ05271 l(2)35Ea
PZ05271-mr15 dp b Gli+, l(2)35Ea-, l(2)35De-,
l(2)35Dh-, BicC+
mr33 cn bw CycE+, l(2)35Di-, l(2)35Df-,
Gli-, l(2)35Ea+
mr45 dp b l(2)35Ea-
Note: The dp b deletion should go distally, cn bw proximally. However 35Di,
35Df and Gli are definitely distal of 35Ea.
k09033 Gli
k09033-mr9 dp b CycE+, l(2)35Di- - Gli-, l(2)35Ea+
k09033-mr19 dp b vasa+, stc- - Gli-, l(2)35Ea+
k14608 l(2)35Fe
k14608-mr4 dp b l(2)35Fe-
mr6 dp b l(2)35Di- - l(2)35Fe-
mr15 dp b fzy- - l(2)35Fe-
mr16 dp b twe- - l(2)35Fe-
mr23 cn bw l(2)35Fe- - chif-
mr27 dp b cact- - l(2)35Fe-
mr29 dp b l(2)35Ff- - l(2)35Fe
Double-P male recombinants - induced over dpov1 b1 cn1 bw1
k07245 - k05605  BG:DS00797.1  - RpII33
k07245L k05605R-mr1 kuz - RpII33
k07245L k05605R-mr3 kuz - RpII33
k07245L k05605R-mr5 l(2)34Db - MtPolB
k07245L k05605R-mr9 l(2)34Db - MtPolB
k07245L k05605R-mr6 l(2)34Db - l(2)34Fa
k07245L k05605R-mr8 l(2)34Db - l(2)34Fa
k07245L k05605R-mr10 l(2)34Db - RpII33
k07245L k05605R-mr12 l(2)34Db - RpII33
k07245L k05605R-mr7 l(2)34Db - RpII33
k07245 - k00811  BG:DS00797.1  - l(2)34Fa
k07245L k00811R-mr2 l(2)34Db - l(2)34Fa
k07245L k00811R-mr3 l(2)34Db - l(2)34Fa
k07245L k00811R-mr4 l(2)34Db - l(2)34Fa
k07245L k00811R-mr5 l(2)34Db - l(2)34Fa
k07245L k00811R-mr11 l(2)34Db - l(2)34Fa
k07245L k00811R-mr13 l(2)34Db - l(2)34Fa
k07245L k00811R-mr14 l(2)34Db - l(2)34Fa
k07245L k00811R-mr6 l(2)34Db - l(2)34Fa
k07245L k00811R-mr15 l(2)34Db - l(2)34Fa
k07245L k00811R-mr7 l(2)34Db - l(2)34Fa
k07245L k00811R-mr8 l(2)34Db - l(2)34Fa
k07245L k00811R-mr10 l(2)34Db - l(2)34Fa
k07245L k00811R-mr16 l(2)34Db - l(2)34Fa
k07245L k00811R-mr17 l(2)34Db - l(2)34Fa
w11P - PZ07130 rk - ck
rkw11PL PZ07130R-mr1 rk - ck
rkw11PL PZ07130R-mr2 rk - ck
rkw11PL PZ07130R-mr3 rk - ck
rkw11PL PZ07130R-mr4 rk - ck
rkw11PL PZ07130R-mr5 rk - ck
rkw11PL PZ07130R-mr6 rk - ck
rkw11PL PZ07130R-mr7 rk - ck
rkw11PL PZ07130R-mr8 rk - ck
rkw11PL PZ07130R-mr9 rk - ck
rkw11PL PZ07130R-mr10 rk - ck
rkw11PL PZ07130R-mr11 rk - ck
rkw11PL PZ07130R-mr12 rk - ck
rkw11PL PZ07130R-mr13 rk - ck
rkw11PL PZ07130R-mr14 rk - ck
rkw11PL PZ07130R-mr15 rk - ck
rkw11PL PZ07130R-mr16 rk - ck