FlyBase Gene Report: Dmel\DCTN5-p25

General Information

Symbol

Dmel\DCTN5-p25

Species

D. melanogaster

Name

Dynactin 5, p25 subunit

Annotation Symbol

CG10846

Feature Type

protein_coding_gene

FlyBase ID

FBgn0040228

Gene Model Status

Current

Stock Availability

10 publicly available

Gene Snapshot

Dynactin 5, p25 subunit (DCTN5-p25) encodes a motor protein involved in spermatogenesis. It also contributes to the dendritic restriction of the product of Dscam1. [Date last reviewed: 2019-08-01]

Other Summaries

Alliance
Auto summary
Gene Group
Red Book
Interactive Fly

Also Known As

l(2)35Bf, l(2)br2, dyn-p25, p25, br2

Key Links

Genomic Location

Cytogenetic map

35B8-35B8

Sequence location

2L:15,005,733..15,006,607 [+]

Recombination map

2-50

RefSeq locus

NT_033779 REGION:15005733..15006607

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (2 terms)

Molecular Function (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in retrograde axonal transport

inferred from mutant phenotype

(Yang et al., 2008)

involved_in spermatogenesis

inferred from mutant phenotype

(Lindsley et al., 2013)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

DYNACTIN COMPLEX

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

DYNACTIN COMPLEX -: Dynactin is a large 1.2 MDa multisubunit protein complex that associates with the cytoplasmic dynein complex to drive microtubule-based transport. (Adapted from PMID:15473859 and PMID:25751425).

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

l(2)35Bf

Escapers show thin, short bristles and outstretched wings. l(2)35Bf3 partially complements l(2)35Bf1 and l(2)35Bf2 for viability; female-sterile alleles recovered by Lindsley.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\DCTN5-p25 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.45

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

DCTN5-p25-RA

FBtr0080693

NM_165097

730

189

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

DCTN5-p25-PA

FBpp0080255

20.6

189

8.44

NP_723893

AAF53426

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\DCTN5-p25 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

dyn-p25_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\DCTN5-p25 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\DCTN5-p25

View exonic expression by tissue for Dmel\DCTN5-p25

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0040228

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG10846-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10846

Flygut - An atlas of the Drosophila adult midgut

FBgn0040228

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 13 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of DCTN5-p25

Transgenic constructs containing regulatory region of DCTN5-p25

Deletions and Duplications ( 103 )

Not disrupted in

Df(2L)A47

(Ashburner et al., 1982)

Df(2L)A63

(Ashburner et al., 1982)

Df(2L)A178

(Ashburner et al., 1982)

Df(2L)A217

(Ashburner et al., 1982)

Df(2L)A245

(Ashburner et al., 1982)

Df(2L)A260

(Ashburner et al., 1982)

Df(2L)A266

(Ashburner et al., 1982)

Df(2L)A379

(Ashburner et al., 1982)

Df(2L)A445

(Ashburner et al., 1982)

Df(2L)Antp^Ctxrv1

(Roote, 1996.8)

Df(2L)b75

(Woodruff and Ashburner, 1979)

Df(2L)b84a7

(Furukawa et al., 1992)

Df(2L)el77

(Woodruff and Ashburner, 1979)

Df(2L)fn2

(Ashburner et al., 1982, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)fn3

(Ashburner, 1982, Ashburner et al., 1982, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)fn7

(Ashburner, 1982, Ashburner et al., 1982, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)fn36

(Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)k08808-rv70

(Roote, 2000.6.13)

Df(2L)rd9

(Furukawa et al., 1992)

Df(2L)Sco-1

(Ashburner, 1982)

Df(2L)TE35B-3

(Gubb et al., 1985)

Df(2L)TE35B-4

(Gubb et al., 1985)

Df(2L)TE35B-7

(Gubb et al., 1985)

Df(2L)TE35B-8

(Gubb et al., 1985)

Df(2L)TE35B-9

(Gubb et al., 1985)

Df(2L)TE35B-10

(Gubb et al., 1985)

Df(2L)TE35B-11

(Gubb et al., 1985)

Df(2L)TE35B-12

(Gubb et al., 1985)

Df(2L)TE35B-15

(Gubb et al., 1985)

Df(2L)TE35BC-1

(Gubb et al., 1984, Ashburner, 1982)

Df(2L)TE35BC-3

(Gubb et al., 1984, Ashburner, 1982)

Df(2L)TE35BC-24

(Iyengar et al., 1999, Gubb et al., 1984)

Df(2L)TE35D-2

(Furukawa et al., 1992)

Df(2L)TE35D-6

(Furukawa et al., 1992)

Df(2L)TE35D-13

(Furukawa et al., 1992)

Df(2L)TE35D-15

(Furukawa et al., 1992)

Df(2L)TE35D-18

(Furukawa et al., 1992)

Df(2L)W

(Woodruff and Ashburner, 1979)

In(2L)75c^LC158.1^R

(Woodruff and Ashburner, 1979)

T(2;3)Sco^rv7

(Woodruff and Ashburner, 1979)

T(Y;2)J165^DP58^P

(Woodruff and Ashburner, 1979)

T(Y;2)R15^DJ165^P

(Woodruff and Ashburner, 1979)

Disrupted in

Df(2L)64j

(Roote, 2010.1.4, Furukawa et al., 1992, Ashburner, 1982, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)A48

(Ashburner et al., 1982)

Df(2L)A72

(Ashburner et al., 1982)

Df(2L)A215

(Ashburner et al., 1982)

Df(2L)A220

(Ashburner et al., 1982)

Df(2L)A246

(Ashburner et al., 1982)

Df(2L)A263

(Furukawa et al., 1992, Ashburner et al., 1982)

Df(2L)A264

(Ashburner et al., 1982)

Df(2L)A267

(Ashburner et al., 1982)

Df(2L)A376

(Ashburner, 1982, Ashburner et al., 1982)

Df(2L)A377

(Ashburner, 1982, Ashburner et al., 1982)

Df(2L)A400

(Roote, 2010.1.4, Ashburner, 1982, Ashburner et al., 1982)

Df(2L)ARR1

(Furukawa et al., 1992, Ashburner, 1982)

Df(2L)b80k

(Roote, 2010.1.4)

Df(2L)b84a3

(Furukawa et al., 1992)

Df(2L)b84h50

(Furukawa et al., 1992)

Df(2L)do1

(Ashburner, 1982)

Df(2L)ED1050

(Roote, 2004.6.24)

Df(2L)el28

(Furukawa et al., 1992)

Df(2L)Exel7061

(Ryder, 2004.4.26)

Df(2L)fn1

(Ashburner et al., 1982, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)fn5

(Furukawa et al., 1992, Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)fn12

(Ashburner et al., 1982, Woodruff and Ashburner, 1979)

Df(2L)fn15

(Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)fn26

(Ashburner et al., 1982)

Df(2L)fn27

(Ashburner, 1982, Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)fn30

(Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)fn31

(Ashburner, 1982, Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)osp29

(Furukawa et al., 1992)

Df(2L)Sco^rv4

(Woodruff and Ashburner, 1979)

Df(2L)Sco^rv23

(Ashburner, 1982)

Df(2L)TE35B-1

(Gubb et al., 1985)

Df(2L)TE35B-2

(Gubb et al., 1985)

Df(2L)TE35B-5

(Gubb et al., 1985)

Df(2L)TE35B-6

(Gubb et al., 1985)

Df(2L)TE35BC-4

(Gubb et al., 1984, Ashburner, 1982)

Df(2L)TE35BC-6

(Gubb et al., 1984)

Df(2L)TE35BC-7

(Furukawa et al., 1992, Gubb et al., 1984)

Df(2L)TE35BC-8

(Gubb et al., 1984)

Df(2L)TE35BC-23

(Gubb et al., 1984)

Df(2L)TE35BC-28

(Furukawa et al., 1992, Gubb et al., 1984)

Df(2L)TE35BC-29

(Gubb et al., 1984)

Df(2L)TE35BC-31

(Gubb et al., 1984)

Df(2L)TE35BC-34

(Gubb et al., 1984)

Df(2L)TE35BC-35

(Gubb et al., 1984)

In(2L)75c

(Ashburner, 1982, Woodruff and Ashburner, 1979)

In(2L)C158^LSco^rv11R

(Ashburner and Harrington, 1984)

In(2L)C158^LSco^rv17R

(Ashburner and Harrington, 1984)

In(2L)TE35B-13

(Gubb et al., 1985)

In(2LR)noc^4LSco^rv9R

(Ashburner and Harrington, 1984)

In(2LR)TE35B-6^LSco^rv9R

(Ashburner and Harrington, 1984)

T(Y;2)A80^DR15^P

(Woodruff and Ashburner, 1979)

Tp(2;2)A446

(Ashburner et al., 1982)

Ts(2Lt;3Lt)TE35B-4+Ts(2Rt;3Rt)Sco^rv13

(Ashburner and Harrington, 1984)

Ts(2Lt;3Lt)TE35BC-3+Ts(2Rt;3Rt)Sco^rv13

(Ashburner and Harrington, 1984)

Duplicated in

Dp(2;1)Sco^rv23

(Ashburner, 1982)

Dp(2;2)GYL

(Ashburner, 1982)

Dp(2;2)GYS

(Ashburner, 1982)

In(2L)C163.41^LC158^R

(Ashburner, 1982)

In(2L)Sco^rv17LC158^R

(Ashburner, 1982)

Not duplicated in

Dp(2;2)Adh3

(Ashburner, 1982)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^pnr-MD237

DCTN5-p25^GD3469

lethal | recessive

partially lethal - majority die | adult stage, with Scer\GAL4^pnr-MD237

DCTN5-p25^GD3469

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

DCTN5-p25^GD3469

viable, with Scer\GAL4^Mef2.PR

DCTN5-p25^GD3469

viable, with Scer\GAL4^tin.CΔ4

DCTN5-p25^GD3469

Sterility

Allele

female sterile

DCTN5-p25⁸

male sterile

DCTN5-p25^Z4556

male sterile (with DCTN5-p25⁴)

DCTN5-p25^Z4556

male sterile (with DCTN5-p25⁸)

DCTN5-p25^Z4556

male sterile (with DCTN5-p25^Z4556)

male sterile (with Df(2L)64j)

DCTN5-p25^Z4556

male sterile (with Df(2L)A400)

DCTN5-p25^Z4556

male sterile (with Df(2L)b80k)

DCTN5-p25^Z4556

Other Phenotypes

Allele

visible, with Scer\GAL4^pnr-MD237

DCTN5-p25^GD3469

Phenotype manifest in

Allele

macrochaeta

macrochaeta & thorax (with Df(2L)64j)

DCTN5-p25^Z4556

macrochaeta & thorax (with Df(2L)A400)

DCTN5-p25^Z4556

mesothoracic tergum, with Scer\GAL4^pnr-MD237

DCTN5-p25^GD3469

wing

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\DCTN5

13 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Dctn5

12 of 15

Yes

Rattus norvegicus (Norway rat) (1)

Rnor\Dctn5

9 of 13

Yes

Xenopus tropicalis (Western clawed frog) (1)

Xtro\dctn5

13 of 12

Yes

Danio rerio (Zebrafish) (1)

Drer\dctn5

9 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\dnc-5

11 of 15

Yes

Arabidopsis thaliana (thale-cress) (7)

Atha\GAMMA CA2

3 of 9

Yes

Atha\GAMMA CA1

2 of 9

Yes

Atha\GAMMA CA3

2 of 9

Yes

Atha\GAMMA CAL1

2 of 9

Yes

Atha\GAMMA CAL2

2 of 9

Yes

Atha\LpxD1

1 of 9

Yes

Atha\LpxD2

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190FXN )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA10595

Drosophila persimilis

Dper\GL26020

Drosophila willistoni

Dwil\GK18306

Drosophila virilis

Dvir\GJ11828

Drosophila mojavensis

Dmoj\GI13204

Drosophila grimshawi

Dgri\GH10910

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150BLX )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17008829

Glossina morsitans

Tsetse fly

Gmor\GMOY006069

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_11783

Mayetiola destructor

Hessian fly

Mdes\Mdes003537

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL007566

Anopheles darlingi

American malaria mosquito

Adar\ADAC009501

Anopheles gambiae

Malaria mosquito

Agam\AGAP013747

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ003121

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0EFD )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA005274

Danaus plexippus

Monarch butterfly

Dple\DPOGS204932-PA

Apis florea

Little honeybee

Aflo\2163580132

Apis mellifera

Western honey bee

Amel\Ame.2266

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP22892

Bombus terrestris

Buff-tailed bumblebee

Bter\15210497

Linepithema humile

Argentine ant

Lhum\LH10994

Linepithema humile

Argentine ant

Lhum\LH10995

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0028bc

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG010823

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_11416

Tribolium castaneum

Red flour beetle

Tcas\GLEAN_08312

Pediculus humanus

Human body louse

Phum\PHUM101570

Rhodnius prolixus

Kissing bug

Rpro\RPRC011459

Cimex lectularius

Bed bug

Clec\CLEC011943

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI004169

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_09099

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0EE8 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR004556

Strigamia maritima

European centipede

Smar\SMAR007399

Ixodes scapularis

Black-legged tick

Isca\ISCW010831

Stegodyphus mimosarum

African social velvet spider

Smim\KFM78522.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur04g03080

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_230296

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_106006

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0NK3 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Dynact_p25ph

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000011630

Gallus gallus

Domestic chicken

Ggal\DCTN5

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (0)

No records found.

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

DCTN5-p25^GD3469

exacerbates tauopathy

modeled by Hsap\MAPT^R406W.UAS

(Butzlaff et al., 2015)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

DCTN5; dynactin subunit 5

13 of 15

612962

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

DCTN5-p25 - Arp1

experimental knowledge based

(Guruharsha et al., 2011)

DCTN5-p25 - Arp10

experimental knowledge based

(Guruharsha et al., 2011)

DCTN5-p25 - DCTN2-p50

experimental knowledge based

(Guruharsha et al., 2011)

DCTN5-p25 - DCTN4-p62

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

60892

DroID - A comprehensive database of gene and protein interactions.

FBgn0040228

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

34873

MIST (protein-protein) - An integrated Molecular Interaction Database

DCTN5-p25

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-50

Cytogenetic map

35B8-35B8

Sequence location

2L:15,005,733..15,006,607 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

35B8-35B8

Limits computationally determined from genome sequence between P{EP}elB^EP2039&P{PZ}osp^rJ571 and P{lacW}Su(H)^k07904

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-50

(FlyBase, 2016)

2-47.8

(Comeron et al., 2012)

Left of (cM)

Su(H)

(Woodruff and Ashburner, 1979)

Right of (cM)

Ankrd49

(Woodruff and Ashburner, 1979)

Notes

Complementation analysis establishes the following gene order (distal to proximal): dyn-p25, l(2)35Be, l(2)35Bc.

(Ashburner et al., 1999)

Excision of l(2)35Bc^k08808 indicates that the gene order is mol, dyn-p25, l(2)35Be, l(2)35Bc, l(2)35Bd.

(Ashburner et al., 1999.10.12)

Stocks and Reagents

Stocks (10)

Bloomington

3214

b¹ Adhⁿ⁴ DCTN5-p25⁵/CyO

30467

Adhⁿ⁷ DCTN5-p25³ cn¹/CyO

10853

w¹¹¹⁸; PBac{PB}DCTN5-p25^c02174/CyO

29643

DCTN5-p25^Z4556 cn¹ bw¹/CyO

83589

y¹ sc^* v¹ sev²¹; P{SAM.dCas9.GS05225}attP40/CyO

30491

y¹ sc^* v¹ sev²¹; P{TRiP.HM05233}attP2/TM3, Sb¹

Kyoto

101301

b¹ Adhⁿ⁴ DCTN5-p25⁵ / CyO, Duox^Cy dpy^lv1 pr¹ cn²

VDRC

v104352

P{KK107799}VIE-260B

v8057

w¹¹¹⁸; P{GD3469}v8057

v8058

w¹¹¹⁸; P{GD3469}v8058

Genomic Clones (18)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (6)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0040228

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0040228

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34873

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: DCTN5-p25 dyn-p25

(Hays et al., 2015.6.30)

Source for database merge of

Source for merge of: dynactin-subunit-p25 BG:DS00929.1

(Bayraktaroglu, 2000.8.7)

Source for merge of: dyn-p25 l(2)35Bf

(Roote, 2004.9.30)

Additional comments

" BG:DS00929.1 " may correspond to "l(2)35Bf" or "l(2)35Be". " BG:DS00929.16 ", " BG:DS00929.1 " or " BG:DS00929.2 " may correspond to "l(2)35Bf".

(Ashburner et al., 1999.10.12)

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 35 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34873

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0040228

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

dyn-p25_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10846

Flygut - An atlas of the Drosophila adult midgut

FBgn0040228

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34873

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC008312

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG10846

modMine - A data warehouse for the modENCODE project

FBgn0040228

Linkouts

BioGRID - A database of protein and genetic interactions.

60892

DroID - A comprehensive database of gene and protein interactions.

FBgn0040228

DRSC - Results frm RNAi screens

FBgn0040228

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0040228

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG10846-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0040228

FlyMine - An integrated database for Drosophila genomics

FBgn0040228

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

34873

MIST (protein-protein) - An integrated Molecular Interaction Database

DCTN5-p25

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (21)

Reported As

Symbol Synonym

BG:DS00929.1

(Ashburner et al., 1999, Ashburner et al., 1999.10.12)

(O'Donnell et al., 1977)

CG10846

(Kondo et al., 2017, Hosono et al., 2015, Keleman et al., 2009.8.5, Perkins et al., 2009, Eckley and Schroer, 2003, Goldstein and Gunawardena, 2000)

DCTN5

(Kollmar, 2015.9.21, Hammesfahr and Kollmar, 2012)

DCTN5-p25

(Transgenic RNAi Project members, 2017-, Hays et al., 2015.6.30)

DS00929.1

(Reese et al., 2000)

P25

(Kollmar and Odronitz, 2006)

br2

(Furukawa et al., 1992, Gubb et al., 1985, Ashburner and Harrington, 1984)

dyn-p25

(Lindsley et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Roote, 2010.1.4, Yang et al., 2008)

l(2)35Bf

(Roote, 2001.5.23, Ashburner et al., 1999, Ashburner et al., 1999.10.12, Iyengar et al., 1999, Roote, 1997.6.6, Spradling et al., 1995)

l(2)br2

(The Moscow Regional Drosophila melanogaster Stock Center, Dubna, Gubb et al., 1984, Ashburner, 1982, Ashburner et al., 1982, Woodruff and Ashburner, 1979)

p25

(Butzlaff et al., 2015, Li et al., 2008, Yang et al., 2008, Parisi et al., 2004)

Name Synonyms

Dynactin 5

(Kondo et al., 2017)

Dynactin 5, p25 subunit

Dynactin subunit p-25

(Deivasigamani et al., 2014)

Dynactin5 p25

(Kollmar, 2015.9.15)

dynactin, p25 subunit

(Tekotte and Davis, 2002)

dynactin-subunit-p25

(Bloomington Drosophila Stock Center, 2004.9.23)

lethal 2 in the black-reduced region

(Woodruff and Ashburner, 1979)

lethal group C4

(O'Donnell et al., 1977)

lethal(2)35Bf

Secondary FlyBase IDs

FBgn0001976
FBgn0028929

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (74)

The following external sites may use different assemblies or annotations than FlyBase.

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