Subject: for fb updates Aubrey, Here is a load of stuff that MA and I have just gone through: New alleles: rkKe1 PM allele from Kerbiniou P strain, discovered by Shelton 35BbAM10 .. call this 35Bb10 with AM10 as synonym. On Df(2L)b74c6, which was caffeine + gamma ray induced by Alexandrov. 35Bbk11524 from Kiss collection, mapped here l(2)35BfQL53 on BicCQL53, ref Schupbach and Weischaus 1991 Gen 129:1119 , mapped here Su(H)E86 isolated and mapped by Cathy Shea (MIT), photoreceptor axon projection defects in optic lobe of 3rd instar larvae. (I don't know how it was induced.) Su(H)1356 ry+ P insert from Delattre et al. 1995 Genetics 141:1407 l(2)35BgPI2 PM allele from Shelton, using PI2 strain rdGJ3 from Glynnis Johnson (this lab), don't know how it was induced. gftGR18 on T(Y;2)TE35B-18 (gamma) esgflg: esgfleabag = fleabag, from Alan Shirras, Lancaster, PM induced from Athens 77Q strain l(2)35DfHL58 reference Schupbach and Weischaus 1986 Dev Biol 113:443 , Table 5 Class 2Di - Tiny eggs - failure in transport. HL58/35Df- escapers are fs and have small bristles. tweDTD22 associated with T(2;4)DTD22 fzy8 on Scorv16 fs(2)35Ec no alleles, overlapping deletion phenotype These are leaky fs: RA5/el28, TE35B-2, TE35B-13, Scorv10, Scorv14, TE35D-24, A246, nBR120, pk-Drv2, pk-Drv3 These are completely fs: RA5/Scorv7, TE35BC-8, TE35D-3, TE35D-11, TE35D-17, TE35D-5, TE35D-12, nco4LD6R fs(2)35Ed no alleles, overlapping deletion phenotype leaky fs: RM5/Sco-7 fs: RM5/TE35D-6, TE35D-2, TE35D-14 Df(2L)TE35D-4 broken in stc; should be in FBrf0085996 needs an stc allele name - stcTE35D4 Cheers, John Subject: update Aubrey, Various things are coming out of our check on Adh region stuff in prep for Gerry's visit. Here's a start: kuzIK5. IK5 is an allele of kuz (=34Da), not 35Da l(2)k05224 recently mapped genetically and molecularly to Sos l(2)k06321 recently mapped molecularly to Sos l(2)k05605 recently mapped genetically and molecularly to l(2)35Dg rk84b1 induced at the same time as b84b1, gamma wb91g2 induced at the same time as osp91g2, EMS l(2)k05612 wb allele l(2)k00805 wb allele l(2)k13507 wb allele John > Subject: Re: for fb updates Ele. This is the story: TE35B-14 and TE35B-4 are 2 inversions derived from TE35B, so they both break in noc. -14 breaks in 42F, just proximal of nec (necrotic), -4 breaks in pk. The recombinant In(2LR)TE35B-14LTE35B-4R carries 3 copies of w+, 1 on the left break and 2 on the right and is deleted for nec. The stock has a zeste eye-colour in a zeste1 mutant background. We selected for red eyed derivatives of this and made stocks. One of these had lost the 2 copies from TE35B-4R and we called it SW250 (spontaneous white) - it had (has) a dominant prickle phenotype. We then foolishly made gamma-ray induced revertants of pkD. There are ~12 of these. I will send you what I know about them soon. J > Subject: pkDrv Name Cytology Genetics pkD1 In(2LR)TE35B-4LTE35B-250R noc pkD pkD1-rv1 In(2LR)+In(2R)43;49F noc pk-sple pkD1-rv2 In(2LR)+Df(2LR) Df(2LR)noc-fs(2)35Ec; pk-sple pkD1-rv3 In(2LR)+Df(2LR) Df(2LR)noc-fs(2)35Ec; pk-sple pkD1-rv4 In(2LR) noc pk pkD1-rv5 In(2LR) noc pk pkD1-rv6 In(2LR)+T(2;3)43;80 noc pk-sple pkD1-rv7 In(2LR)+In(2)het noc pk-sple pkD1-rv8 In(2LR)+Df(2LR) Df(2LR)noc-stc;pk-cn pkD1-rv9 In(2LR) noc pk-sple pkD1-rv10 In(2LR)+Df(2LR) Df(2LR)noc-l(2)35Da; pk-l(2)43Ef pkD1-rv11 In(2LR)SW251L+Df(2R) noc Df(2R)pk--scra pkD1-rv12 In(2LR)+Df(2R) noc Df(2R)pk--l(2)43Bb SW251 is a white eyed derivative of: In(2LR)TE35B-4L TE35B-SW250R (NB TE35B-4L not TE35B-14)