FlyBase Gene Report: Dmel\THG

General Information

Symbol

Dmel\THG

Species

D. melanogaster

Name

tRNA-histidine guanylyltransferase

Annotation Symbol

CG4103

Feature Type

protein_coding_gene

FlyBase ID

FBgn0283659

Gene Model Status

Current

Stock Availability

8 publicly available

Enzyme Name (EC)

tRNA(His) guanylyltransferase (2.7.7.79)

Gene Summary

Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage. (UniProt, Q9V3N8)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

BG:DS00929.4 , l(2)br4, br4, l(2)br11, l(2)br13

Key Links

Genomic Location

Cytogenetic map

35B4-35B8

Sequence location

2L:15,008,489..15,009,724 [-]

Recombination map

2-50

RefSeq locus

NT_033779 REGION:15008489..15009724

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (4 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables magnesium ion binding

inferred from electronic annotation with InterPro:IPR007537, InterPro:IPR024956

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables tRNA guanylyltransferase activity

inferred from biological aspect of ancestor with PANTHER:PTN000977687

(assigned by GO_Central )

(Gaudet et al., 2011)

inferred from sequence or structural similarity with UniProtKB:P53215

(assigned by UniProt )

(UniProt Curators, 2002-)

inferred from sequence or structural similarity with UniProtKB:Q9NWX6

(assigned by UniProt )

(UniProt Curators, 2002-)

Biological Process (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in tRNA modification

inferred from sequence or structural similarity with UniProtKB:P53215

(assigned by UniProt )

(UniProt Curators, 2002-)

involved_in tRNA processing

inferred from sequence or structural similarity with UniProtKB:Q9NWX6

(assigned by UniProt )

(UniProt Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN000977687

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

GUANYLYLTRANSFERASES

Protein Family (UniProt)

Belongs to the tRNA(His) guanylyltransferase family. (Q9V3N8)

Protein Signatures (InterPro)

Catalytic Activity (EC)

Experimental Evidence

Predictions / Assertions

p-tRNA(His) + ATP + GTP + H(2)O = pG-P-tRNA(His) + AMP + 2 diphosphate (2.7.7.79)

Summaries

Gene Group (FlyBase)

GUANYLYLTRANSFERASES -: Guanylyltransferases catalyse the transfer of a guanylyl group to an acceptor. Many eukaryotic guanylyl transferases are capping enzymes that catalyze the formation of the 5' cap in messenger RNAs. Others specifically add a guanine nucleotide to the transfer RNA for histidine, which is required for it to be aminoacylated by the histidine tRNA synthetase. Guanylyl transferases also exist for transferring guanosine nucleotides to sugar molecules, such as mannose and fucose. (Adapted from https://en.wikipedia.org/wiki/Guanylyltransferase.)

Protein Function (UniProtKB)

Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage.

(UniProt, Q9V3N8)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\THG for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.45

Sequence Ontology: Class of Gene

gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

THG-RA

FBtr0080727

NM_135893

1149

286

THG-RB

FBtr0303561

NM_001201886

1076

286

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

THG-PA

FBpp0080286

33.1

286

7.60

NP_609737

AAF53429

THG-PB

FBpp0292594

33.1

286

7.60

NP_001188815

ADV37065

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

286 aa isoforms: THG-PA, THG-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9V3N8

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\THG using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

l(2)35Bc_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\THG in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\THG

View exonic expression by tissue for Dmel\THG

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB

34876/tissue=All

EMBL-EBI Single Cell Expression Atlas

FBgn0283659

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 9 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of THG

Transgenic constructs containing regulatory region of THG

Aberrations (Deficiencies and Duplications) ( 112 )

Inferred from experimentation ( 112 )

Gene not disrupted in

Df(2L)A47

(Gubb et al., 1990, Ashburner et al., 1982)

Df(2L)A63

(Ashburner et al., 1982)

Df(2L)A178

(Ashburner et al., 1982)

Df(2L)A217

(Ashburner et al., 1982)

Df(2L)A245

(Ashburner et al., 1982)

Df(2L)A260

(Ashburner et al., 1982)

Df(2L)A266

(Ashburner et al., 1982)

Df(2L)A379

(Ashburner et al., 1982)

Df(2L)A445

(Ashburner et al., 1982)

Df(2L)b75

(Gubb et al., 1990, Woodruff and Ashburner, 1979)

Df(2L)b84a7

(Furukawa et al., 1992)

Df(2L)el77

(Woodruff and Ashburner, 1979)

Df(2L)fn2

(Ashburner et al., 1982, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)fn3

(Ashburner, 1982, Ashburner et al., 1982, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)fn7

(Ashburner, 1982, Ashburner et al., 1982, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)fn36

(Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)GT4

(Gubb et al., 1990)

Df(2L)rd9

(Furukawa et al., 1992)

Df(2L)Sco-1

(Ashburner, 1982)

Df(2L)TE35B-3

(Gubb et al., 1985)

Df(2L)TE35B-4

(Gubb et al., 1985)

Df(2L)TE35B-7

(Gubb et al., 1985)

Df(2L)TE35B-8

(Gubb et al., 1985)

Df(2L)TE35B-9

(Gubb et al., 1985)

Df(2L)TE35B-10

(Gubb et al., 1985)

Df(2L)TE35B-11

(Gubb et al., 1985)

Df(2L)TE35B-12

(Gubb et al., 1985)

Df(2L)TE35B-15

(Gubb et al., 1985)

Df(2L)TE35BC-1

(Gubb et al., 1984, Ashburner, 1982)

Df(2L)TE35BC-3

(Gubb et al., 1984, Ashburner, 1982)

Df(2L)TE35BC-24

(Iyengar et al., 1999, Gubb et al., 1984)

Df(2L)TE35D-2

(Furukawa et al., 1992)

Df(2L)TE35D-6

(Furukawa et al., 1992)

Df(2L)TE35D-13

(Furukawa et al., 1992)

Df(2L)TE35D-15

(Furukawa et al., 1992)

Df(2L)TE35D-18

(Furukawa et al., 1992)

Df(2L)W

(Woodruff and Ashburner, 1979)

In(2L)75c^LC158.1^R

(Woodruff and Ashburner, 1979)

T(2;3)Sco^rv7

(Woodruff and Ashburner, 1979)

T(Y;2)a15

(Davis et al., 1997)

T(Y;2)el⁴

(Davis et al., 1997)

T(Y;2)J165^DP58^P

(Woodruff and Ashburner, 1979)

T(Y;2)R15^DJ165^P

(Woodruff and Ashburner, 1979)

Gene disrupted in

Df(2L)64j

(Furukawa et al., 1992, Ashburner, 1982, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)A48

(Gubb et al., 1990, Ashburner et al., 1982)

Df(2L)A72

(Ashburner et al., 1982)

Df(2L)A215

(Ashburner et al., 1982)

Df(2L)A220

(Ashburner et al., 1982)

Df(2L)A246

(Ashburner et al., 1982)

Df(2L)A263

(Furukawa et al., 1992, Ashburner et al., 1982)

Df(2L)A264

(Ashburner et al., 1982)

Df(2L)A267

(Ashburner et al., 1982)

Df(2L)A376

(Ashburner, 1982, Ashburner et al., 1982)

Df(2L)A377

(Ashburner, 1982, Ashburner et al., 1982)

Df(2L)A400

(Ashburner, 1982, Ashburner et al., 1982)

Df(2L)ARR1

(Furukawa et al., 1992, Ashburner, 1982)

Df(2L)b84a3

(Furukawa et al., 1992)

Df(2L)b84h50

(Furukawa et al., 1992)

Df(2L)do1

(Ashburner, 1982)

Df(2L)el28

(Furukawa et al., 1992)

Df(2L)Exel7061

(Ryder, 2004.4.26)

Df(2L)fn1

(Ashburner et al., 1982, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)fn5

(Furukawa et al., 1992, Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)fn12

(Ashburner et al., 1982, Woodruff and Ashburner, 1979)

Df(2L)fn15

(Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)fn26

(Ashburner et al., 1982)

Df(2L)fn27

(Ashburner, 1982, Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)fn30

(Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)fn31

(Ashburner, 1982, Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)GT5

(Gubb et al., 1990)

(Roote, 2002.2.18)

(Roote, 2000.6.13)

(Roote, 2000.6.13)

(Roote, 2000.6.13)

(Roote, 2000.6.13)

(Roote, 2000.6.13)

(Furukawa et al., 1992, Chia et al., 1985)

Df(2L)Sco^rv4

(Woodruff and Ashburner, 1979)

Df(2L)Sco^rv23

(Ashburner, 1982)

Df(2L)TE35B-1

(Gubb et al., 1985)

Df(2L)TE35B-2

(Gubb et al., 1985)

Df(2L)TE35B-5

(Gubb et al., 1985)

Df(2L)TE35B-6

(Gubb et al., 1985)

Df(2L)TE35BC-4

(Gubb et al., 1984, Ashburner, 1982)

Df(2L)TE35BC-6

(Gubb et al., 1984)

Df(2L)TE35BC-7

(Furukawa et al., 1992, Gubb et al., 1984)

Df(2L)TE35BC-8

(Gubb et al., 1984)

Df(2L)TE35BC-23

(Gubb et al., 1984)

Df(2L)TE35BC-28

(Furukawa et al., 1992, Gubb et al., 1984)

Df(2L)TE35BC-29

(Gubb et al., 1984)

Df(2L)TE35BC-31

(Gubb et al., 1984)

Df(2L)TE35BC-34

(Gubb et al., 1984)

Df(2L)TE35BC-35

(Gubb et al., 1984)

In(2L)75c

(Ashburner, 1982, Woodruff and Ashburner, 1979)

In(2L)C158^LSco^rv11R

(Ashburner and Harrington, 1984)

In(2L)C158^LSco^rv17R

(Ashburner and Harrington, 1984)

In(2L)TE35B-13

(Gubb et al., 1985)

In(2LR)noc^4LSco^rv9R

(Ashburner and Harrington, 1984)

In(2LR)TE35B-6^LSco^rv9R

(Ashburner and Harrington, 1984)

T(2;3)TE35B-2a

(Chia et al., 1985)

T(2;3)TE35B-3^DG16^P

(Chia et al., 1985)

T(Y;2)A80^DR15^P

(Woodruff and Ashburner, 1979)

Tp(2;2)A446

(Ashburner et al., 1982)

Ts(2Lt;3Lt)Mpe+Ts(2Rt;3Rt)G16

(Chia et al., 1985)

Ts(2Lt;3Lt)TE35B-4+Ts(2Rt;3Rt)Sco^rv13

(Chia et al., 1985, Ashburner and Harrington, 1984)

Ts(2Lt;3Lt)TE35BC-3+Ts(2Rt;3Rt)Sco^rv13

(Ashburner and Harrington, 1984)

Gene duplicated in

Dp(2;1)Sco^rv23

(Ashburner, 1982)

Dp(2;2)GYL

(Ashburner, 1982)

Dp(2;2)GYS

(Ashburner, 1982)

In(2L)C163.41^LC158^R

(Ashburner, 1982)

In(2L)Sco^rv17LC158^R

(Ashburner, 1982)

Gene not duplicated in

Dp(2;2)Adh3

(Ashburner, 1982)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

lethal | recessive

lethal - all die before end of P-stage, with Scer\GAL4^nub.PU

THG^GD11217

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

THG^GD11217

viable, with Scer\GAL4^pnr-MD237

THG^GD11217

viable, with Scer\GAL4^tin.CΔ4

THG^GD11217

Other Phenotypes

Allele

abnormal neuroanatomy | larval stage

THG^GD11217

visible, with Scer\GAL4^pnr-MD237

THG^GD11217

Phenotype manifest in

Allele

ganglion mother cell | larval stage

THG^GD11217

mesothoracic tergum, with Scer\GAL4^pnr-MD237

THG^GD11217

trichogen cell, with Scer\GAL4^pnr-MD237

THG^GD11217

wing, with Scer\GAL4^salm.EPv

THG^GD11217

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\THG1L

15 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Thg1l

14 of 15

Yes

Rattus norvegicus (Norway rat) (1)

Rnor\Thg1l

13 of 13

Yes

Xenopus tropicalis (Western clawed frog) (2)

Xtro\thg1l

9 of 12

Yes

Xtro\LOC101733043

3 of 12

Yes

Danio rerio (Zebrafish) (1)

Drer\thg1l

10 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (0)

No records found.

Arabidopsis thaliana (thale-cress) (2)

Atha\AT2G31580

4 of 9

Yes

Atha\AT2G32320

4 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\THG1

14 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\SPCC63.07

11 of 12

Yes

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190C49 )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA17959

Drosophila persimilis

Dper\GL25730

Drosophila willistoni

Dwil\GK18307

Drosophila virilis

Dvir\GJ16007

Drosophila mojavensis

Dmoj\GI17333

Drosophila grimshawi

Dgri\GH10821

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150A1W )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17012693

Glossina morsitans

Tsetse fly

Gmor\GMOY000580

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_05382

Mayetiola destructor

Hessian fly

Mdes\Mdes009017

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL014040

Anopheles darlingi

American malaria mosquito

Adar\ADAC008325

Anopheles gambiae

Malaria mosquito

Agam\AGAP011752

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ000684

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W06AG )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA002973

Danaus plexippus

Monarch butterfly

Dple\DPOGS211096-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL012475

Apis florea

Little honeybee

Aflo\2905658036

Apis mellifera

Western honey bee

Amel\GB45435

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP21006

Bombus terrestris

Buff-tailed bumblebee

Bter\15211286

Linepithema humile

Argentine ant

Lhum\LH19180

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:002040

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG007456

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_08138

Tribolium castaneum

Red flour beetle

Tcas\TC014454

Pediculus humanus

Human body louse

Phum\PHUM410370

Rhodnius prolixus

Kissing bug

Rpro\RPRC004969

Cimex lectularius

Bed bug

Clec\CLEC012833

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI006122

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_18509

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0AR4 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR008080

Strigamia maritima

European centipede

Smar\SMAR003177

Ixodes scapularis

Black-legged tick

Isca\ISCW001226

Stegodyphus mimosarum

African social velvet spider

Smim\KFM81248.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur27g02090

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_39947

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0GYS )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_008521gn

Ciona intestinalis

Vase tunicate

Cint\LOC100179812

Gallus gallus

Domestic chicken

Ggal\THG1L

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (0)

No records found.

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

THG1L; tRNA-histidine guanylyltransferase 1 like

15 of 15

618802

SPINOCEREBELLAR ATAXIA, AUTOSOMAL RECESSIVE 28; SCAR28

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

THG

two hybrid

(Hu et al., 2017.6.13)

THG - snf

two hybrid

(Hu et al., 2017.6.13)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Smox

suppressible

THG

(Hevia et al., 2017)

External Data

Linkouts

MIST (protein-protein) - An integrated Molecular Interaction Database

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-50

Cytogenetic map

35B4-35B8

Sequence location

2L:15,008,489..15,009,724 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

35B4-35B8

Left limit from recombination mapping relative to mol (FBrf0033164) Right limit from recombination mapping relative to l(2)35Bd (FBrf0033164); Limits computationally determined from genome sequence between P{EP}elB^EP2039&P{PZ}osp^rJ571 and P{lacW}Su(H)^k07904

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

35B6-35B10

(determined by in situ hybridisation)

(Ashburner et al., 1999, Spradling et al., 1999)

35B6-35B10

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

2-50

(FlyBase, 2016)

2-47.8

(Comeron et al., 2012)

2-[50]

Left of (cM)

Su(H)

(Ashburner, 1982)

Rnmt

(Woodruff and Ashburner, 1979)

Right of (cM)

(Ashburner, 1982, Woodruff and Ashburner, 1979)

(Woodruff and Ashburner, 1979)

Notes

Complementation analysis establishes the following gene order (distal to proximal): dyn-p25, l(2)35Be, l(2)35Bc.

(Ashburner et al., 1999)

Excision of l(2)35Bc^k08808 indicates that the gene order is mol, dyn-p25, l(2)35Be, l(2)35Bc, l(2)35Bd.

(Ashburner et al., 1999.10.12)

Stocks and Reagents

Stocks (8)

Bloomington

10810

y¹ w^67c23; P{lacW}THG^k08808/CyO

30465

Adhⁿ¹ THG⁴ PolD3^rd-s pr¹ cn¹ l(2)CA2^HG1/CyO

3772

b¹ THG¹ pr¹/In(2L)Cy, In(2R)Cy, Duox^Cy Bl¹ cn²

Kyoto

101305

b¹ THG⁶ Adhⁿ⁴ / CyO

111247

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{lacW}THG^k08808 P{neoFRT}40A/CyO y⁺

101300

b¹ Adhⁿ² THG² pr¹ cn¹ / CyO, Duox^Cy dpy^lv1 pr¹ cn²

VDRC

v105438

P{KK102440}VIE-260B

v26390

w¹¹¹⁸; P{GD11217}v26390

Genomic Clones (21)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (9)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

IP10135

Other clones

DPiM_IP10135

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0283659

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: BG:DS00929.4 CG4103

(Kodira, 1999.11)

Source for identity of: THG l(2)35Bc

(FlyBase, 1992-)

Source for database merge of

Source for merge of: l(2)35Bc CG4103

(Huen and Roote, 2010.5.4)

Source for merge of: tRNA-guanylyltransferase l(2)35Bc

(FlyBase, 1992-)

Additional comments

"l(2)35Bc" probably corresponds to either " BG:DS00929.4 " or to " BG:DS00929.3 ".

(Ashburner et al., 1999)

" BG:DS00929.2 ", " BG:DS00929.3 " or " BG:DS00929.4 " may correspond to "l(2)35Bc".

(Ashburner et al., 1999.10.12)

" BG:DS00929.4 " may correspond to "l(2)35Bc".

(Ashburner et al., 1999.10.12)

The non-sequence localized gene 'tRNA-guanylyltransferase' (FBgn0023421), associated only with FBrf0052523, and the sequence localized gene 'l(2)35Bc/CG4103' (FBgn0261882) have been merged because the latter is the only CG-gene identified as a tRNA-guanylyltransferase (by protein domains or GO annotation). The symbol and full-name of the merged gene are based on the nomenclature used for this enzyme in other species.

(FlyBase, 1992-)

Other Comments

tRNA-guanylyltransferase enzyme has been purified and characterised.

(Williams et al., 1990)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 22 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34876

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9V3N8

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0283659

DRscDB

34876/tissue=All

EMBL-EBI Single Cell Expression Atlas

FBgn0283659

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

l(2)35Bc_1

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG4103

MARRVEL_MODEL

34876

Linkouts

MIST (protein-protein) - An integrated Molecular Interaction Database

Synonyms and Secondary IDs (19)

Reported As

Symbol Synonym

BG:DS00929.4

(Giot et al., 2003, Ashburner et al., 1999, Ashburner et al., 1999, Ashburner et al., 1999.10.12)

(O'Donnell et al., 1977)

CG4103

(Hevia et al., 2017, Hosono et al., 2015, Japanese National Institute of Genetics, 2012.5.21, Huen and Roote, 2010.5.4, Keleman et al., 2009.8.5)

THG

(Hu et al., 2017.6.13)

br4

(Furukawa et al., 1992, Gubb et al., 1985, Ashburner and Harrington, 1984)

l(2)35Bc

(Huen and Roote, 2010.5.4)

l(2)br

(Ashburner, 1982)

l(2)br11

(Woodruff and Ashburner, 1979)

l(2)br13

(Woodruff and Ashburner, 1979)

l(2)br4

(Gubb et al., 1984, Ashburner et al., 1982, Ashburner et al., 1982, Woodruff and Ashburner, 1979)

l(2)k08808

tRNA-guanylyltransferase

Name Synonyms

lethal (2) 35Bc

lethal 11 in the black-reduced region

(Woodruff and Ashburner, 1979)

lethal 13 in the black-reduced region

(Woodruff and Ashburner, 1979)

lethal 4 in the black-reduced region

(Woodruff and Ashburner, 1979)

lethal group C1

(O'Donnell et al., 1977)

tRNA guanylyltransferase

(Williams et al., 1990)

tRNA-histidine guanylyltransferase

Secondary FlyBase IDs

FBgn0023421
FBgn0261882
FBgn0001973
FBgn0028925

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (52)

Report Sections

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