FlyBase Insertion Report: Dmel\P{PZ}ck[07130]

General Information

Symbol

Dmel\P{PZ}ck⁰⁷¹³⁰

Species

D. melanogaster

Name

FlyBase ID

FBti0004834

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

Uses

lacZ reporter

Affected gene(s)

ck, Ecol\lacZ

Viability / fertility

Associated allele(s)

ck⁰⁷¹³⁰, Ecol\lacZ^ck-07130

Stock availability

1 publicly available

LINE ID

l(2)07130

Genomic Location

Chromosomal Location

2L ( 35B9 )

Sequence Location

2L:15,057,843..15,057,843 [+]

Target / Docking site

Genomic Maps

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Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2L:15,057,843..15,057,843 [+]

(BDGP Project Members, 1994-1999)

Orientation

Cytological location (computed by FlyBase)

35B9 (inferred by FlyBase from sequence location)

Cytological location (reported)

35C1-35C3 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AQ025664

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Location-dependent role

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

(Rudrapatna et al., 2014, BDGP Project Members, 1994-1999)

Ecol\lacZ

(Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

Alleles and Phenotypes

Allele

ck⁰⁷¹³⁰

Reference(s)

(Rudrapatna et al., 2014, Todi et al., 2005, Kiehart et al., 2004, Ashburner et al., 1999, Ashburner et al., 1999, Ashburner et al., 1999.10.12, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Phenotype Data

Phenotypic Class Phenotype Manifest in Detailed Description

Allele

Ecol\lacZ^ck-07130

Reference(s)

(Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

Phenotype Data

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

In wandering third instar larva, no detectable β-gal staining.

(Meister and Braun, 1995.10)

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)07130

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

12337

cn¹ P{PZ}ck⁰⁷¹³⁰/CyO; ry⁵⁰⁶

External Crossreferences and Linkouts ( 0 )

Comments

Location 2L:15053772-15053773 confirmed by FlyBase alignment of dbGSS accession AQ025664 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.

(FlyBase, 2005)

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

P(PZ)07130

(Kiehart et al., 2004)

P2337

(Meister and Braun, 1995.10)

P{PZ}07130

(Ashburner et al., 1999)

P{PZ}ck⁰⁷¹³⁰

(FlyBase, 2005, BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{PZ}l(2)07130⁰⁷¹³⁰

(FlyBase, 1992-)

P{PZ}l(2)35Bd⁰⁷¹³⁰

(FlyBase, 1992-)

P{ry^+t7.2=PZ}l(2)35Bd⁰⁷¹³⁰

(FlyBase, 1992-)

ck⁰⁷¹³⁰

(Rudrapatna et al., 2014)

Name Synonyms

Secondary FlyBase IDs

FBti0000444

References (18)

Report Sections

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