FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\chif
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General Information
Symbol
Dmel\chif
Species
D. melanogaster
Name
chiffon
Annotation Symbol
CG5813
Feature Type
protein_coding_gene
FlyBase ID
FBgn0000307
Gene Model Status
Current
Stock Availability
21 publicly available
Gene Summary
chiffon (chif) encodes a protein associated with the origin recognition complex that binds to chromosomal origins of replication and is required for the initiation of cellular DNA replication. It is involved in DNA replication, chorion production, muscle homeostasis, and axon guidance. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)
All Summaries
Also Known As

BG:DS09218.1 , Dbf4, BG:DS09218.2

Key Links
Genomic Location
Cytogenetic map
35F1-35F12
Sequence location
Recombination map
2-52
RefSeq locus
NT_033779 REGION:16342430..16352628
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (14 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables protein kinase activator activity
inferred from direct assay
(Stephenson et al., 2015)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
enables nucleic acid binding
inferred from electronic annotation with InterPro:IPR006572
(InterPro Project Members, 2004-)
enables protein serine/threonine kinase activator activity
inferred from biological aspect of ancestor with PANTHER:PTN002310337
(GO Reference Genome Project, 2011-)
enables zinc ion binding
inferred from electronic annotation with InterPro:IPR006572
(InterPro Project Members, 2004-)
Biological Process (8 terms)
Terms Based on Experimental Evidence (6 terms)
CV Term
Evidence
References
involved_in chorion-containing eggshell formation
inferred from high throughput mutant phenotype
(Schupbach and Wieschaus, 1991)
involved_in chromatin remodeling
inferred from direct assay
(Torres-Zelada et al., 2019)
involved_in eggshell chorion gene amplification
inferred from mutant phenotype
(Stephenson et al., 2015, Claycomb et al., 2004, Zhang and Tower, 2004, Landis and Tower, 1999)
involved_in muscle cell cellular homeostasis
inferred from mutant phenotype
(Kucherenko et al., 2011)
inferred from genetic interaction with FLYBASE:Dg; FB:FBgn0034072
(Kucherenko et al., 2011)
inferred from genetic interaction with FLYBASE:Dys; FB:FBgn0260003
(Kucherenko et al., 2011)
involved_in photoreceptor cell axon guidance
inferred from mutant phenotype
(Marrone et al., 2011)
involved_in positive regulation of DNA replication
inferred from mutant phenotype
(Stephenson et al., 2015)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in positive regulation of nuclear cell cycle DNA replication
inferred from biological aspect of ancestor with PANTHER:PTN002310337
(GO Reference Genome Project, 2011-)
involved_in regulation of cell cycle phase transition
inferred from biological aspect of ancestor with PANTHER:PTN002310337
(GO Reference Genome Project, 2011-)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
part_of Dbf4-dependent protein kinase complex
inferred from direct assay
(Stephenson et al., 2015)
part_of H3 histone acetyltransferase complex
inferred from direct assay
(Torres-Zelada et al., 2019)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
part_of Dbf4-dependent protein kinase complex
inferred from biological aspect of ancestor with PANTHER:PTN002310337
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
chiffon (chif) encodes a protein associated with the origin recognition complex that binds to chromosomal origins of replication and is required for the initiation of cellular DNA replication. It is involved in DNA replication, chorion production, muscle homeostasis, and axon guidance. [Date last reviewed: 2019-03-07]
Gene Group (FlyBase)
CHAT COMPLEX -
The chiffon histone acetyltransferase (CHAT) complex is a unique Gcn5 complex specific to insects that shows characteristic histone acetyltransferase activity towards histone H3. (Adapted from FBrf0241207, FBrf0247978 and FBrf0248705).
DBF4-DEPENDENT CDC7 KINASE COMPLEX -
The Dbf4-dependent Cdc7 kinase complex is a serine/threonine-protein kinase that phosphorylates components of the pre-replication complex during DNA replication initiation. (Adapted from FBrf0227432.)
Protein Function (UniProtKB)
A bicistronic gene producing two proteins that are components of different complexes and have separate properties and functions (PubMed:30559249). Full-length protein is proteolytically cleaved, producing a 50kDa N-terminal product (Chiffon-A) that forms part of the DDK complex; it is unclear if the C-terminal proteolytic product is stable or functional (Probable). Alternative initiation from an internal ribosome entry site produces a C-terminal 48kDa product (Chiffon-B or Isoform E) that forms part of the CHAT complex (PubMed:30559249). Involved in regulation of gene expression during embryonic development (PubMed:34908116).
(UniProt, Q9NK54)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
chif: chiffon (T. Schupbach)
Female sterile, homozygous females lay eggs which have thin, fragile chorions and remain unfertilized.
Summary (Interactive Fly)

Dbf4 homolog - regulatory subunit of Cdc7-Dbf4 dimer - associated with the origin recognition complex activates the kinase function of its dimerization partner, thus triggering the initiation of cellular DNA replication

Gene Model and Products
Number of Transcripts
4
Number of Unique Polypeptides
3

Please see the JBrowse view of Dmel\chif for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9NK54)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Multiphase exon postulated: this gene shares a region of coding sequence with an overlapping gene, but different reading frames are utilized in the overlapping coding region; overlap >20aa.

Gene model reviewed during 5.43

Gene model reviewed during 5.50

Complex/atypical overlap: Shares 5' UTR with downstream gene; in addition, one transcript isoform shares 3' UTR with downstream gene.

Gene model reviewed during 6.27

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
chif-RA
FBtr0080838
NM_078859
6624
1711
chif-RB
FBtr0080839
NM_165156
6575
1695
chif-RD
FBtr0321313
NM_001273570
6170
1695
chif-RE
FBtr0475053
NM_001382183
6575
576
Additional Transcript Data and Comments
Reported size (kB)

6.6, 6.5 (northern blot)

(Landis and Tower, 1999)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
chif-PA
FBpp0080396
189.2
1711
8.73
Q9NK54
NP_523583
AAF53530
chif-PB
FBpp0080397
188.0
1695
8.92
M9PD16
NP_723965
AAG22437
chif-PD
FBpp0302856
188.0
1695
8.92
M9PD16
NP_001260499
AGB93034
chif-PE
FBpp0423267
63.6
576
4.79
QJC20912
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1695 aa isoforms: chif-PB, chif-PD
Additional Polypeptide Data and Comments
Reported size (kDa)

1711, 1695 (aa)

(Landis and Tower, 1999)
Comments
External Data
Post Translational Modification

May be proteolytically cleaved to produce a N-terminal 50 kDa product.

(UniProt, Q9NK54)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\chif using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.92

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism | ubiquitous

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
head mesoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
mesoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 7 -- 8
antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
visual anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
ventral ectoderm
(Fisher et al., 2012)
embryonic stage 9 -- 10
antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 11 -- 12
anterior midgut primordium
(Fisher et al., 2012)
central brain primordium
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
posterior midgut primordium
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 13 -- 16
embryonic brain
(Fisher et al., 2012)
embryonic/larval midgut
(Fisher et al., 2012)
organism | faint | ubiquitous
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
larval ventral nerve cord
(Fisher et al., 2012)
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 2
organism
(Landis and Tower, 1999)
adult stage & oogenesis
nurse cell
(Landis and Tower, 1999)
follicle cell
(Landis and Tower, 1999)
adult stageoogenesis stage S10 -- S11
(Landis and Tower, 1999)
adult stageoogenesis stage S13 -- S14
(Landis and Tower, 1999)
Additional Descriptive Data

Abundant chif transcripts are detected in nurse cells in egg chambers. Low levels of chif transcripts are detected in follicle cells. chif transcripts are detected in egg chambers and very early embryos on northern blots indicating that they are maternally supplied to the embryo.

(Landis and Tower, 1999)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
day 5 of adulthood | male -- day 7 of adulthood | male
spermatozoon
(Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
part_of Dbf4-dependent protein kinase complex
inferred from direct assay
(Stephenson et al., 2015)
part_of H3 histone acetyltransferase complex
inferred from direct assay
(Torres-Zelada et al., 2019)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\chif in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 21 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 15 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of chif
Transgenic constructs containing regulatory region of chif
Aberrations (Deficiencies and Duplications) ( 67 )
Inferred from experimentation ( 67 )
Gene disrupted in
Df(2L)BSC781
(Kahsai and Cook, 2018, Kahsai et al., 2016, Christensen et al., 2009.5.6)
Df(2L)cact-255rv64
(Dawson et al., 1995, Tower et al., 1993)
Df(2L)Exel7065
(Ryder, 2004.4.26)
Df(2L)k04216-rv2
(Roote, 2000.6.13)
Df(2L)k04216-rv3
(Roote, 2000.6.13)
Df(2L)k08106-rv4
(Roote, 2000.6.13)
Df(2L)k08106-rv6
(Roote, 2000.6.13, Roote, 1999.7.7)
Df(2L)k08106-rv15
(Roote, 2000.6.13, Roote, 1999.7.7)
Df(2L)k14608-mr23
(Roote, 2000.6.13)
Df(2L)k17027rv2
(Roote, 2000.6.13)
Df(2L)r10
(Schupbach and Wieschaus, 1991, Ashburner et al., 1990)
Df(2L)RA5
(Levine et al., 2007, Eberl et al., 1998, Roote, 1996.8, Ashburner et al., 1990)
Df(2L)RM5
(Ashburner et al., 1990)
Df(2L)RN2
(Eberl et al., 1998, Dawson et al., 1995, Ashburner et al., 1990)
Df(2L)TE35D-16
(Ashburner et al., 1990)
Df(2L)TE35D-21
(Roote, 1996.8, Dawson et al., 1995, Ashburner et al., 1990)
Df(2L)TE35D-23
(Ashburner et al., 1990)
Gene not disrupted in
Df(2L)64j
(Ashburner et al., 1990)
Df(2L)A72
(Ashburner et al., 1990)
Df(2L)A267
(Ashburner et al., 1990)
Df(2L)ARR1
(Ashburner et al., 1990)
Df(2L)b80e4
(Ashburner et al., 1990)
Df(2L)el20
(Ashburner et al., 1990)
Df(2L)el28
(Ashburner et al., 1990)
Df(2L)fn12
(Ashburner et al., 1990)
Df(2L)fn26
(Ashburner et al., 1990)
Df(2L)fn27
(Ashburner et al., 1990)
Df(2L)H20
(Ashburner et al., 1990)
Df(2L)H60-3
(Roote, 1996.1.11, Roote, 1996.8, Dawson et al., 1995)
Df(2L)II30
(Roote, 1996.8)
Df(2L)III18
(Roote, 1996.8)
Df(2L)MN-I
(Levine et al., 2007)
Df(2L)n78l3
(Ashburner et al., 1990)
Df(2L)noc11
(Ashburner et al., 1990)
Df(2L)osp29
(Eberl et al., 1998)
Df(2L)osp38
(Ashburner et al., 1990)
Df(2L)rd9
(Ashburner et al., 1990)
Df(2L)Sco7
(Ashburner et al., 1990)
Df(2L)Scorv16
(Ashburner et al., 1990)
Df(2L)Scorv18
(Ashburner et al., 1990)
Df(2L)Scorv19
(Ashburner et al., 1990)
Df(2L)Scorv25
(Ashburner et al., 1990)
Df(2L)SS1
(Ashburner et al., 1990)
Df(2L)TE35BC-3
(Ashburner et al., 1990)
Df(2L)TE35BC-4
(Ashburner et al., 1990)
Df(2L)TE35BC-34
(Ashburner et al., 1990)
Df(2L)TE35D-1
(Ashburner et al., 1990)
Df(2L)TE35D-2
(Ashburner et al., 1990)
Df(2L)TE35D-3
(Ashburner et al., 1990)
Df(2L)TE35D-4
(Ashburner et al., 1990)
Df(2L)TE35D-6
(Ashburner et al., 1990)
Df(2L)TE35D-13
(Eberl et al., 1998, Dawson et al., 1995, Ashburner et al., 1990)
Df(2L)TE35D-14
(Eberl et al., 1998, Ashburner et al., 1990)
Df(2L)TE35D-15
(Ashburner et al., 1990)
Df(2L)TE35D-19
(Ashburner et al., 1990)
Df(2L)TE35D-20
(Ashburner et al., 1990)
Df(2L)TE35D-22
(Ashburner et al., 1990)
In(2L)dpps22Ldppd36R
(Ashburner et al., 1990)
In(2L)el18
(Eberl et al., 1998, Dawson et al., 1995, Ashburner et al., 1990)
In(2LR)Scorv1-DV7
(Ashburner et al., 1990)
T(2;3)Scorv7
(Ashburner et al., 1990)
Gene duplicated in
Dp(2;3)osp3
(Ashburner et al., 1990)
Gene not duplicated in
Dp(2;1)Scorv23
(Ashburner et al., 1990)
Dp(2;2)GYL
(Ashburner et al., 1990)
Dp(2;2)TE35B-54L
(Ashburner et al., 1990)
In(2L)C163.41LC158R
(Ashburner et al., 1990)
In(2L)Scorv17LC158R
(Ashburner et al., 1990)
Inferred from location ( 7 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal (with chifDsRed)
lethal (with chifETBE3)
lethal (with Df(2L)RA5)
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Sterility
Allele
female sterile
female sterile (with Df(2L)BSC781)
female sterile | recessive
fertile, with chif1-375.Tag:HA,Tag:FLAG, chifDsRed/chifETBE3
fertile, with chif401-1695.Tag:HA,Tag:FLAG, chifDsRed/chifETBE3
fertile, with chifDsRed/chifETBE3
fertile, with Df(2L)RA5/chifDsRed
fertile, with Df(2L)RA5/chifETBE3
fertile (with chifDsRed), with chif1-375.Tag:HA,Tag:FLAG, chif401-1695.Tag:HA,Tag:FLAG
fertile (with chifDsRed), with chif1-1695.Tag:HA,Tag:FLAG
fertile (with chifDsRed), with chif376aa.Tag:HA,Tag:FLAG
fertile (with chifETBE3), with chif1-375.Tag:HA,Tag:FLAG, chif401-1695.Tag:HA,Tag:FLAG
fertile (with chifETBE3), with chif1-1695.Tag:HA,Tag:FLAG
fertile (with chifETBE3), with chif376aa.Tag:HA,Tag:FLAG
fertile (with Df(2L)RA5), with chif1-1695.Tag:HA,Tag:FLAG
fertile (with Df(2L)RA5), with chif376aa.Tag:HA,Tag:FLAG
sterile, with chifDsRed/chifETBE3
sterile, with Df(2L)RA5/chifDsRed
sterile, with Df(2L)RA5/chifETBE3
sterile (with chifDsRed), with chif401-1695.Tag:HA,Tag:FLAG
sterile (with chifDsRed), with chifWF24.Tag:HA,Tag:FLAG
sterile (with chifETBE3), with chif401-1695.Tag:HA,Tag:FLAG
sterile (with chifETBE3), with chifWF24.Tag:HA,Tag:FLAG
sterile (with Df(2L)RA5), with chif401-1695.Tag:HA,Tag:FLAG
sterile (with Df(2L)RA5), with chifWF24.Tag:HA,Tag:FLAG
Other Phenotypes
Allele
abnormal locomotor behavior | dominant | temperature conditional
abnormal neuroanatomy | third instar larval stage
decreased occurrence of cell division | somatic clone
increased cell death | oogenesis | somatic clone
short lived | heat sensitive, with Scer\GAL4tin.CΔ4
visible, with Scer\GAL4pnr-MD237
visible | recessive
wild-type
Phenotype manifest in
Allele
axon | third instar larval stage
egg chorion
eye
follicle cell | somatic clone
indirect flight muscle cell (with chifA507)
indirect flight muscle cell (with chifBG02820a)
indirect flight muscle cell (with chifEY05746)
lamina plexus | third instar larval stage
oocyte | somatic clone
scutellar bristle
trichogen cell, with Scer\GAL4pnr-MD237
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (2)
Hsap\DBF4B
7 of 14
Yes
Yes
Hsap\DBF4
7 of 14
Yes
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (1)
Rnor\Dbf4
4 of 14
Yes
Yes
Mus musculus (laboratory mouse) (1)
Mmus\Dbf4
5 of 14
Yes
Yes
Xenopus tropicalis (Western clawed frog) (4)
Xtro\dbf4b
4 of 13
Yes
Yes
Xtro\dbf4
4 of 13
Yes
Yes
Xtro\gpr4
1 of 13
No
Yes
Xtro\xpo6
1 of 13
No
No
Danio rerio (Zebrafish) (2)
Drer\dbf4
6 of 14
Yes
Yes
Drer\dbf4b
5 of 14
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (0)
Anopheles gambiae (African malaria mosquito) (1)
Agam\AgaP_AGAP012023
6 of 12
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\DBF4
4 of 13
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (2)
Spom\dfp1
5 of 12
Yes
Yes
Spom\spo6
3 of 12
No
Yes
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:chif. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 2 )
    Allele
    Disease
    Interaction
    References
    chifBG02820a
    ameliorates  muscular dystrophy
    modeled by DgO86
    (Marrone et al., 2011)
    exacerbates  muscular dystrophy
    modeled by DgO86
    (Kucherenko et al., 2011)
    modeled by Dg323
    (Kucherenko et al., 2011)
    chifEY05746
    exacerbates  muscular dystrophy
    modeled by DgO86
    (Kucherenko et al., 2011)
    modeled by DgRNAi.UAS
    (Kucherenko et al., 2011)
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    DBF4B; DBF4B-CDC7 kinase regulatory subunit
    7 of 14
    611661
        DBF4; DBF4-CDC7 kinase regulatory subunit
        7 of 14
        604281
            Functional Complementation Data
            Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
            Interactions
            Summary of Physical Interactions
            Summary of Genetic Interactions
            Interaction Browsers
            View in FlyBase Interactions Browser   View in MIST tool (external link)
            Please look at the allele data for full details of the genetic interactions
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            Dg
            enhanceable
            chif
            (Kucherenko et al., 2011, Kucherenko et al., 2008)
            External Data
            Linkouts
            DroID - A comprehensive database of gene and protein interactions.
            MIST (genetic) - An integrated Molecular Interaction Database
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Pathways
            Signaling Pathways (FlyBase)
            Metabolic Pathways
            FlyBase
            External Links
            External Data
            Linkouts
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            Class of Gene
            Genomic Location and Detailed Mapping Data
            Chromosome (arm)
            2L
            Recombination map
            2-52
            Cytogenetic map
            35F1-35F12
            Sequence location
            FlyBase Computed Cytological Location
            Cytogenetic map
            Evidence for location
            35F1-35F12
            Experimentally Determined Cytological Location
            Cytogenetic map
            Notes
            References
            35F-36A
            (Landis, 1999.6.10)
            35F11-35F12
            (determined by in situ hybridisation)
            (Ashburner et al., 1999)
            35F11-35F12
            (BDGP Project Members, 1994-1999)
            Experimentally Determined Recombination Data
            Location

            2-52

            (FlyBase, 2016)

            2-49.7

            (Comeron et al., 2012)

            2-53

            Left of (cM)
            Right of (cM)
            Notes
            Stocks and Reagents
            Stocks (21)
            Genomic Clones (30)
            cDNA Clones (50)
             

            Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

            cDNA clones, fully sequenced
            BDGP DGC clones
            Other clones
              Drosophila Genomics Resource Center cDNA clones

              For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

              cDNA Clones, End Sequenced (ESTs)
              BDGP DGC clones
              Other clones
              RNAi and Array Information
              Linkouts
              DRSC - Results frm RNAi screens
              Antibody Information
              Laboratory Generated Antibodies
               

              polyclonal

              (Torres-Zelada et al., 2022)
              Commercially Available Antibodies
               
              Cell Line Information
              Publicly Available Cell Lines
               
                Other Stable Cell Lines
                 
                  Other Comments

                  The Hrp59 protein binds preferentially to a subset of mRNAs, including chif mRNA.

                  (Kiesler et al., 2005)

                  Hypomorphic mutations in chif cause thin, fragile chorions and female sterility, and eliminate chorion gene amplification. Null alleles have the additional phenotypes of rough eyes and thin thoracic bristles; phenotypes often associated with disruption of the normal cell cycle.

                  (Landis and Tower, 1999)

                  chif is involved in processes in addition to chorion gene amplification.

                  (Landis and Tower, 1995)

                  Mutations at the chif locus cause defects in midoogenesis.

                  (Schupbach and Wieschaus, 1991)

                  5 alleles are discussed but are not named.

                  (Ashburner et al., 1990)
                  Relationship to Other Genes
                  Source for database merge of

                  Source for merge of: chif CG5817

                  (FlyBase, 1996-)
                  Additional comments

                  Sequences corresponding to the 5' end of release 2 CG18115 overlap the 3' end of release 3 chif transcript CG5813-RA.

                  (FlyBase Genome Annotators, 2002-2003)

                  Release 1 annotation CG5817 overlaps part of the release 3.2 annotation for chif (CG5813).

                  (FlyBase, 1996-)
                  Nomenclature History
                  Source for database identify of
                  Nomenclature comments
                  Etymology
                  Synonyms and Secondary IDs (12)
                  Datasets (0)
                  Study focus (0)
                  Experimental Role
                  Project
                  Project Type
                  Title
                  Study result (0)
                  Result
                  Result Type
                  Title
                  External Crossreferences and Linkouts ( 64 )
                  Sequence Crossreferences
                  NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                  GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                  GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                  RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                  UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                  UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
                  Other crossreferences
                  AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                  BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                  DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                  EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                  FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                  FlyMine - An integrated database for Drosophila genomics
                  InterPro - A database of protein families, domains and functional sites
                  KEGG Genes - Molecular building blocks of life in the genomic space.
                  MARRVEL_MODEL - MARRVEL (model organism gene)
                  Linkouts
                  Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                  DroID - A comprehensive database of gene and protein interactions.
                  DRSC - Results frm RNAi screens
                  Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                  FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                  FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                  Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                  Flygut - An atlas of the Drosophila adult midgut
                  FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
                  iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                  Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
                  MIST (genetic) - An integrated Molecular Interaction Database
                  MIST (protein-protein) - An integrated Molecular Interaction Database
                  Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                  References (103)