FB2026_03 , released September 17, 2026

CV Term Report

Help Close All Open All

General Information

Term

multidimensional protein identification technology

ID (Ontology)

MI:0658 (Molecular Interactions)

Definition

MudPIT is a method for rapid and large-scale protein identification by multidimensional liquid chromatography associated with tandem mass spectrometry. The chromatography step consists of strong cation exchange material back-to-back with reversed phase material inside fused silica capillaries. The peptides bound to the cation-exchange resin are freed by the gradually increasing salt concentration of the buffer and are subsequently retained by the reversed phase resin. Increasing buffers hydrophobicity progressively elute peptides from the reversed phase packing directly into the mass spectrometer. Typically this mass spectrometer will be a tandem electrospray, so peptides undergo ionization in the liquid phase, are separated in a primary mass spectrometer, analysed in the second mass spectrometer and identified.[ PubMed:11231557 ]

Also Known As

"mudpit"

Comment

Links to External Ontologies

Annotations

Records annotated with this term OR any of its CHILD TERMS

Choose a record type to view annotations for this term and its child terms. Direct annotations and full annotation statements below also provide HitList buttons.

Results list data from multiple species. Choose a record type above to open its HitList.

Direct annotations: records annotated with this exact term (child terms are NOT included)

There are no annotations using this term.

Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records

Full annotation statements	Relevant FlyBase reports
Full annotation statements	Interaction Groups
multidimensional protein identification technology (all annotations which use CV term, excluding "NOT" statements)

Spanning Tree (Parents/Children)

predetermined participant
 |__confirmation by molecular weight_____
experimental participant identification  |
 |__protein sequence identification______|
 |__Identification by mass spectrometry__|
                                         multidimensional protein identification technology  39 rec.

Spanning Tree View Settings
Parents/Children View Depth	Show hierarchy levels: for parents, for children
Relationships
Is a	protein sequence identification Identification by mass spectrometry confirmation by molecular weight
Part of
Synonyms & Secondary IDs
Synonyms
	"mudpit" EXACT PSI-MI-short
Secondary IDs

External Crossreferences & Linkouts