N-terminal domain chif (1-375aa) is expressed under chif 3' and 5' regulatory elements. Tagged at the N-terminal with 2xTag:HA and at the C-terminal with Tag:FLAG.
fertile, with chif401-1695.Tag:HA,Tag:FLAG, chifDsRed/chifETBE3
chif1-375.Tag:HA,Tag:FLAG rescues chifETBE3
chif1-375.Tag:HA,Tag:FLAG/chif401-1695.Tag:HA,Tag:FLAG rescues chifDsRed/chifETBE3
chif1-375.Tag:HA,Tag:FLAG fails to rescue chifDsRed/chifETBE3
chif1-375.Tag:HA,Tag:FLAG fails to rescue Df(2L)RA5/chifETBE3
chif1-375.Tag:HA,Tag:FLAG fails to rescue Df(2L)RA5/chifDsRed