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General Information
Symbol
Dmel\b1
Species
D. melanogaster
Name
FlyBase ID
FBal0000858
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
b
Key Links
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Comment:

Four bases (ATCC) have been replaced by an eight base pair insertion (TACCTACC), causing a frameshift. One of two changes affecting the coding sequence identified in mutant strain.

Inserted_sequence:

TACCTACC

Nucleotide change:

T13822961C

Reported nucleotide change:

T1042C

Amino acid change:

Y348H | b-PA; Y348H | b-PB; Y287H | b-PC

Reported amino acid change:

Y348H

Comment:

One of two changes affecting the coding sequence identified in mutant strain.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

There is an amino acid replacement in a conserved residue (Y348H, nucleotide substitution T1042C) compared to the wild-type sequence, and, in addition, upstream of the amino acid replacement, four bases (ATCC) have been replaced by an eight base pair insertion (TACCTACC) at nucleotide position +530, resulting in a frameshift that is expected to produce a truncated, enzymatically-inactive protein.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

b1 flies have a normal electroretinogram. b1 flies show no difference in walking speed or walking distance compared to wild-type flies when assayed in Buridan's paradigm. However, the number of walks initiated by b1 flies is significantly different from that of wild-type flies and fixation behaviour is defective in b1 flies in this assay.

When unpigmented b1 pharate adult tissue is incubated with dopamine in vitro, the extent of inducible pigmentation of body cuticle, hairs and bristle sockets resembles that of wild-type, while pigmentation of the bristle shafts is significantly reduced.

Levels of β-alanine transaminase activity are elevated compared to wild-type in newly emerged adults up to five hours postemergence. Levels of β-alanine transaminase activity are higher in su(b)6 ; b1 double mutant flies than in su(b)6 single mutant flies. Levels of β-alanine transaminase activity in su(b)6; b1 double mutant flies are lower than wild-type levels.

Black pigment on body and tarsi and along wing veins, darkening with age. Heterozygote shows somewhat darker trident, but is never confused with homozygote. Puparium usually somewhat lighter than wild type and newly emerged flies not clearly distinguishable from wild type. RK1 in aged flies.

Body colour: black. RK1 in aged flies.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement
Reference

pyd3Lb5/b1 is an enhancer of visible | heat sensitive phenotype of Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU

b1 is an enhancer of abnormal body color phenotype of Streak1

Suppressor of
Statement
Reference

pyd3+t3.8, pyd3Lb10, b1 is a suppressor | partially of visible | heat sensitive phenotype of Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU

b1 is a suppressor of visible | heat sensitive phenotype of Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU

NOT Suppressor of
Statement
Reference

b1 is a non-suppressor of abnormal neurophysiology phenotype of e11

Other
Phenotype Manifest In
NOT suppressed by
Statement
Reference

b1 has phenotype, non-suppressible by su(Hw)2

Enhancer of
Statement
Reference

b1 is an enhancer of scutellum phenotype of Streak1

Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference

b1 is a non-suppressor of retina phenotype of e11

Additional Comments
Genetic Interactions
Statement
Reference

su(r)1, rC; b1 mutants show wild-type freezing responses in 60% of flies and exhibit a delayed response in only 20% of flies.

b1 ; pyd3Lb5 enhances the wing blistering phenotype seen in the posterior compartment of wings in flies expressing EogtGD5084 under the control of Scer\GAL4en.PU in the presence of Dcr-2Scer\UAS.cDa.

b1 ; pyd3+t3.8 ; pyd3Lb10 partially suppresses the wing blistering phenotype seen in the posterior compartment of wings in flies expressing EogtGD5084 under the control of Scer\GAL4en.PU in the presence of Dcr-2Scer\UAS.cDa at 27[o]C.

The wing blistering phenotype seen in the posterior compartment of wings in flies expressing EogtGD5084 under the control of Scer\GAL4en.PU in the presence of Dcr-2Scer\UAS.cDa is robustly suppressed at 27[o]C if the flies also carry b1.

The electroretinogram phenotype of b1 ; e11 double mutant flies is identical to that of e11 single mutants.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (1)
Stocks (551)
Notes on Origin
Discoverer

Morgan, 1910.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
References (22)