FlyBase Allele Report: Dmel\Adc[b-1]

General Information

Symbol

Dmel\Adc^b-1

Species

D. melanogaster

Name

FlyBase ID

FBal0000858

Feature type

allele

Associated gene

Dmel\Adc

Associated Insertion(s)

Carried in Construct

Also Known As

b¹, b

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

spontaneous

Nature of the Allele

Allele class

loss of function allele

(Lindsley and Grell, 1968)

Mutagen

spontaneous

(Ashburner et al., 1999.10.12, Lindsley and Grell, 1968)

Progenitor genotype

Cytology

Description

There is an amino acid replacement in a conserved residue (Y348H, nucleotide substitution T1042C) compared to the wild-type sequence, and, in addition, upstream of the amino acid replacement, four bases (ATCC) have been replaced by an eight base pair insertion (TACCTACC) at nucleotide position +530, resulting in a frameshift that is expected to produce a truncated, enzymatically-inactive protein.

(Phillips et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

2L:13,821,916..13,821,919 [+]

Comment:

Four bases (ATCC) have been replaced by an eight base pair insertion (TACCTACC), causing a frameshift. One of two changes affecting the coding sequence identified in mutant strain.

Inserted_sequence:

TACCTACC

(Phillips et al., 2005)

point_mutation

2L:13,822,961..13,822,961 [+]

Nucleotide change:

T13822961C

Reported nucleotide change:

T1042C

Amino acid change:

Y348H | Adc-PA; Y348H | Adc-PB; Y287H | Adc-PC

Reported amino acid change:

Y348H

Comment:

One of two changes affecting the coding sequence identified in mutant strain.

(Phillips et al., 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body color

(Lindsley and Grell, 1968)

abnormal visual behavior

(Phillips et al., 2005)

viable

(Phillips et al., 2005)

Phenotype Manifest In

adult cuticle

(Lindsley and Grell, 1968)

puparium cuticle

(Lindsley and Grell, 1968)

tarsal segment

(Lindsley and Grell, 1968)

trident

(Lindsley and Grell, 1968)

wing vein

(Lindsley and Grell, 1968)

Detailed Description

Statement

Reference

b¹ flies have a normal electroretinogram. b¹ flies show no difference in walking speed or walking distance compared to wild-type flies when assayed in Buridan's paradigm. However, the number of walks initiated by b¹ flies is significantly different from that of wild-type flies and fixation behaviour is defective in b¹ flies in this assay.

(Phillips et al., 2005)

When unpigmented b¹ pharate adult tissue is incubated with dopamine in vitro, the extent of inducible pigmentation of body cuticle, hairs and bristle sockets resembles that of wild-type, while pigmentation of the bristle shafts is significantly reduced.

(Walter et al., 1996)

Levels of β-alanine transaminase activity are elevated compared to wild-type in newly emerged adults up to five hours postemergence. Levels of β-alanine transaminase activity are higher in su(b)⁶ ; b¹ double mutant flies than in su(b)⁶ single mutant flies. Levels of β-alanine transaminase activity in su(b)⁶; b¹ double mutant flies are lower than wild-type levels.

(Weber et al., 1992)

Black pigment on body and tarsi and along wing veins, darkening with age. Heterozygote shows somewhat darker trident, but is never confused with homozygote. Puparium usually somewhat lighter than wild type and newly emerged flies not clearly distinguishable from wild type. RK1 in aged flies.

(Lindsley and Grell, 1968)

Body colour: black. RK1 in aged flies.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

pyd3^Lb5/Adc^b-1 is an enhancer of visible | heat sensitive phenotype of Dcr-2^UAS.cDa, Eogt^GD5084, Scer\GAL4^en.PU

(Müller et al., 2013)

Adc^b-1 is an enhancer of abnormal body color phenotype of Streak¹

(Lindsley and Zimm, 1992)

Suppressor of

Statement

Reference

pyd3^+t3.8, pyd3^Lb10, Adc^b-1 is a suppressor | partially of visible | heat sensitive phenotype of Dcr-2^UAS.cDa, Eogt^GD5084, Scer\GAL4^en.PU

(Müller et al., 2013)

Adc^b-1 is a suppressor of visible | heat sensitive phenotype of Dcr-2^UAS.cDa, Eogt^GD5084, Scer\GAL4^en.PU

(Müller et al., 2013)

NOT Suppressor of

Statement

Reference

Adc^b-1 is a non-suppressor of abnormal neurophysiology phenotype of e¹¹

(Phillips et al., 2005)

Other

Statement

Reference

Adc^b-1, r^C, su(r)¹ has abnormal neurophysiology phenotype

(Chaturvedi et al., 2014)

Adc^b-1, r^C, su(r)¹ has abnormal visual behavior phenotype

(Chaturvedi et al., 2014)

Adc^b-1, Eogt^GD5084, su(r)¹ has lethal phenotype

(Müller et al., 2013)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Adc^b-1 has phenotype, non-suppressible by su(Hw)²

(Lindsley and Grell, 1968)

Enhancer of

Statement

Reference

pyd3^Lb5/Adc^b-1 is an enhancer of wing blade posterior compartment | heat sensitive phenotype of Dcr-2^UAS.cDa, Eogt^GD5084, Scer\GAL4^en.PU

(Müller et al., 2013)

Adc^b-1 is an enhancer of scutellum phenotype of Streak¹

(Lindsley and Zimm, 1992)

Suppressor of

Statement

Reference

Adc^b-1 is a suppressor of wing blade posterior compartment | heat sensitive phenotype of Dcr-2^UAS.cDa, Eogt^GD5084, Scer\GAL4^en.PU

(Müller et al., 2013)

NOT Suppressor of

Statement

Reference

Adc^b-1 is a non-suppressor of retina phenotype of e¹¹

(Phillips et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

su(r)¹, r^C; b¹ mutants show wild-type freezing responses in 60% of flies and exhibit a delayed response in only 20% of flies.

(Chaturvedi et al., 2014)

b¹ ; pyd3^Lb5 enhances the wing blistering phenotype seen in the posterior compartment of wings in flies expressing Eogt^GD5084 under the control of Scer\GAL4^en.PU in the presence of Dcr-2^Scer\UAS.cDa.

b¹ ; pyd3^+t3.8 ; pyd3^Lb10 partially suppresses the wing blistering phenotype seen in the posterior compartment of wings in flies expressing Eogt^GD5084 under the control of Scer\GAL4^en.PU in the presence of Dcr-2^Scer\UAS.cDa at 27[o]C.

The wing blistering phenotype seen in the posterior compartment of wings in flies expressing Eogt^GD5084 under the control of Scer\GAL4^en.PU in the presence of Dcr-2^Scer\UAS.cDa is robustly suppressed at 27[o]C if the flies also carry b¹.

(Müller et al., 2013)

The electroretinogram phenotype of b¹ ; e¹¹ double mutant flies is identical to that of e¹¹ single mutants.

(Phillips et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (1)

Holtzman, S. and Kaufman, T. (2013)

Mutant

Wild-type