FlyBase Gene Report: Dmel\moody

General Information

Symbol

Dmel\moody

Species

D. melanogaster

Name

moody

Annotation Symbol

CG4322

Feature Type

protein_coding_gene

FlyBase ID

FBgn0025631

Gene Model Status

Current

Stock Availability

14 publicly available

Gene Summary

Isoform A and isoform B are required in glia to regulate the acute sensitivity to cocaine and to continuously maintain the proper blood-brain barrier (BBB) function. A moody-mediated signaling pathway functions in glia to regulate nervous system insulation and drug-related behaviors. Galphai and Galphao, and the regulator of G protein signaling, loco, are required in the surface glia to achieve effective insulation. The components function by regulating the cortical actin and thereby stabilizing the extended morphology of the surface glia, which in turn is necessary for the formation of septate junctions of sufficient length to achieve proper sealing of the nerve cord. (UniProt, Q9W534)

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All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

EG:22E5.11 , EP1631

Key Links

Genomic Location

Cytogenetic map

2C7-2C8

Sequence location

X:2,044,292..2,050,120 [-]

Recombination map

1-0.5

RefSeq locus

NC_004354 REGION:2044292..2050120

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (13 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables G protein-coupled receptor activity

inferred from mutant phenotype

(Bainton et al., 2005)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables G protein-coupled receptor activity

inferred from electronic annotation with InterPro:IPR000276

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity

(Brody and Cravchik, 2000)

inferred from biological aspect of ancestor with PANTHER:PTN001200905

(GO Reference Genome Project, 2011-)

Biological Process (9 terms)

Terms Based on Experimental Evidence (9 terms)

CV Term

Evidence

References

involved_in axon ensheathment

inferred from mutant phenotype

(Bainton et al., 2005)

involved_in behavioral response to cocaine

inferred from mutant phenotype

(Bainton et al., 2005)

involved_in behavioral response to ethanol

inferred from mutant phenotype

(Bainton et al., 2005)

involved_in behavioral response to nicotine

inferred from mutant phenotype

(Bainton et al., 2005)

involved_in cortical actin cytoskeleton organization

inferred from mutant phenotype

(Schwabe et al., 2005)

involved_in establishment of glial blood-brain barrier

inferred from mutant phenotype

(Stork et al., 2008, Bainton et al., 2005, Schwabe et al., 2005)

involved_in G protein-coupled receptor signaling pathway

inferred from mutant phenotype

(Bainton et al., 2005)

involved_in septate junction assembly

inferred from mutant phenotype

(Schwabe et al., 2005)

involved_in ventral cord development

inferred from mutant phenotype

(Schwabe et al., 2005)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in G protein-coupled receptor signaling pathway

inferred from biological aspect of ancestor with PANTHER:PTN001200905

(GO Reference Genome Project, 2011-)

inferred from sequence or structural similarity

(Brody and Cravchik, 2000)

inferred from electronic annotation with InterPro:IPR000276

(InterPro Project Members, 2004-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in plasma membrane

inferred from direct assay

(Schwabe et al., 2005)

located_in pleated septate junction

inferred from direct assay

(Mayer et al., 2009)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

located_in membrane

inferred from electronic annotation with InterPro:IPR000276, InterPro:IPR017452

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity

(Brody and Cravchik, 2000)

Gene Group (FlyBase)

CLASS A GPCR NEUROPEPTIDE AND PROTEIN HORMONE RECEPTORS

Protein Family (UniProt)

Belongs to the G-protein coupled receptor 1 family. (Q9W534)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

CLASS A GPCR NEUROPEPTIDE AND PROTEIN HORMONE RECEPTORS -: The majority of neuropeptide/protein hormone GPCR belong to the Class A family of GPCRs. The secreted ligands for this subfamily include neuropeptides, peptide hormones and protein hormones. (Adapted from FBrf0195077).

Protein Function (UniProtKB)

(UniProt, Q9W534)

Summary (Interactive Fly)

G-protein coupled receptor - expressed in glia and required for blood-brain barrier formation

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\moody for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9W534)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.50

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.42

Multiphase exon postulated: exon reading frame differs in alternative transcripts; overlap >20aa.

Multiphasic exon (use of alternative reading frames in different transcripts) supported (FBrf0189897)

Gene model reviewed during 5.55

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

moody-RA

FBtr0070341

NM_130614

2941

670

moody-RB

FBtr0303043

NM_001201605

3782

670

moody-RC

FBtr0303044

NM_001201606

2939

633

moody-RD

FBtr0303045

NM_001201607

3780

633

moody-RE

FBtr0310288

NM_001272241

2937

670

moody-RF

FBtr0310289

NM_001272242

3014

670

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

moody-PA

FBpp0070327

71.9

670

9.37

FBpp0292162

71.9

670

9.37

FBpp0292163

69.0

633

8.93

FBpp0292164

69.0

633

8.93

FBpp0301971

71.9

670

9.37

FBpp0301972

71.9

670

9.37

Q9W534

NP_001259171

AGB95017

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

670 aa isoforms: moody-PA, moody-PB, moody-PE, moody-PF

633 aa isoforms: moody-PC, moody-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\moody using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.87

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 9

embryonic hemocyte primordium

(Kunwar et al., 2003)

posterior endoderm

(Kunwar et al., 2003)

longitudinal trunk visceral muscle primordium

(Kunwar et al., 2003)

embryonic stage 9 -- 10

anterior endoderm

(Fisher et al., 2012)

crystal cell primordium

Comment: reported as crystal cell specific anlage

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

plasmatocyte primordium

Comment: reported as plasmatocytes anlage

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

embryonic stage 13

fused midgut primordium

(Kunwar et al., 2003)

larval glial cell

(Kunwar et al., 2003)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic central brain

(Fisher et al., 2012)

embryonic central brain glial cell

(Fisher et al., 2012)

embryonic central brain neuron

(Fisher et al., 2012)

embryonic central brain surface glial cell

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

larval nervous system

(Fisher et al., 2012)

embryonic lateral cord

(Fisher et al., 2012)

larval lateral VNC glial cell

(Fisher et al., 2012)

lateral cord neuron

(Fisher et al., 2012)

larval lateral VNC surface glial cell

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

dorsal median cell

(Kearney et al., 2004)

larval B-subperineurial glial cell