DTKR, Takr99D, l(3)s2222, tachykinin receptor
G-protein coupled receptor activated by tachykinin-related peptides - regulates stress induced insulin production and signaling in renal tubules, insulin-producing cells in the adult brain, and presynaptic suppression and olfactory behaviors in the antennal lobe of the brain
Gene model reviewed during 5.44
Stop-codon suppression (UGA) postulated; FBrf0216884.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.55
3.0 (northern blot)
None of the polypeptides share 100% sequence identity.
519 (aa); 57 (kD predicted)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\TkR99D using the Feature Mapper tool.
TkR99D is expressed in most midline neurons with the highest levels in median neuroblast MNB interneuron.
RT-PCR detects TkR99D transcript expression in adult heads and in isolated antennae.
Northern blots show that TkR99D transcripts are expressed throughout development with the highest levels in mid-late embryos and early larvae. In adults, expression is seen predominantly in the head. In situ hybridization in embryos shows that TkR99D transcripts are expressed in the embryonic brain and in a subset of neurons in each neuromere of the developing ventral ganglion. Hybridization to tissue sections of adults shows the strongest hybridization in the cortex of the brain which contains neuronal cell bodies. Little or no hybridization is observed in the central neuropil. Hybridization is also seen in the adult ventral ganglion.
TkR99D protein is detected in the adult and larval brain, in the cell bodies and in varicose processes. In the adult, 7 bilateral cell bodies are labelled: two pairs in the dorsolateral protocerebrum, three pairs in the anterior mediodorsal protocerebrum, one pair in the medial protocerebrum and one pair in the subesophageal ganglion. TkR99D is detected in varicose processes in the antennal lobe, fan-shaped body, pars intecerebralis and medulla. In the larval brain, TkR99D is strongly expressed in the cell body and processes of four anterior neurons. These neurons arborise in the brain, cross the midline and descend into all the neuromeres of the ventral nerve cord. TkR99D is also detected in type II Malpighian tubule cells.
GBrowse - Visual display of RNA-Seq signalsView Dmel\TkR99D in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: TkR99D Takr99D
Xenopus oocyte injection assays suggest that this gene encodes a member of the neurokinin receptor family.