FlyBase Allele Report: Dmel\TkR99D[f02797]

General Information

Symbol

Dmel\TkR99D^f02797

Species

D. melanogaster

Name

FlyBase ID

FBal0159681

Feature type

allele

Associated gene

Dmel\TkR99D

Associated Insertion(s)

PBac{WH}TkR99D^f02797

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

piggyBac activity

Nature of the Allele

Allele class

Mutagen

piggyBac activity

(Bloomington Drosophila Stock Center, 2004.11.6)

Progenitor genotype

Associated Insertion(s)

PBac{WH}TkR99D^f02797

Cytology

Description

Allele components

Component

Use(s)

Inserted element

PBac{WH}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:29,958,398..29,958,398 [-]

(Gu et al., 2022, Bloomington Drosophila Stock Center, 2004.11.6)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

larval multidendritic class IV neuron | third instar larval stage | conditional (with TkR99D^MB09356)

(Im et al., 2015)

Detailed Description

Statement

Reference

TkR99D^f02797/TkR99D^f02797 early third instar larvae do not have significantly different baseline thermal nociception (uninjured larval responses to thermal probes at 45[o] or 48[o]C) compared to controls. 24 hours after UV-induced nociceptive sensitization, early third instar TkR99D^f02797/TkR99D^f02797, TkR99D^f02797/TkR99D^MB09356 or TkR99D^f02797/Df(3R)Exel6213 larvae show significant reduction of thermal allodynia (withdrawal responses to a sub-threshold 38[o]C probe) compared to wild type or heterozygote larvae.

TkR99D^f02797/TkR99D^MB09356 third instar larval fillets do not show a UV-induced increase in action potential firing rate at higher temperatures (36-39[o]C) in class IV neurons, as seen in controls.

(Im et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

TkR99D^f02797/TkR99D^MB09356 is rescued by Scer\GAL4^ppk.1.9/TkR99D^UAS.EGFP

(Im et al., 2015)

Comments

Expression of TkR99D^{Scer\UAS.T:Avic\GFP-EGFP} driven by Scer\GAL4^ppk.1.9 significantly rescues the reduction of thermal allodynia (withdrawal responses to a sub-threshold 38[o]C probe after UV-induced nociceptive sensitization) seen in early third instar TkR99D^f02797/TkR99D^MB09356 larvae. TkR99D^{Scer\UAS.T:Avic\GFP-EGFP} driven by Scer\GAL4^ppk.1.9 significantly restores a UV-induced increase in action potential firing rate in class IV neurons at higher temperatures (36-39[o]C) in third instar TkR99D^f02797/TkR99D^MB09356 larval fillets.

(Im et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Takr99D^f02797

TkR99D^f02797

(Gu et al., 2022)

dtkr^f02797

(Im et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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