FlyBase Allele Report: Dmel\TkR99D[s2222]

General Information

Symbol

Dmel\TkR99D^s2222

Species

D. melanogaster

Name

FlyBase ID

FBal0011147

Feature type

allele

Associated gene

Dmel\TkR99D

Associated Insertion(s)

P{lacW}TkR99D^s2222

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

Progenitor genotype

Associated Insertion(s)

P{lacW}TkR99D^s2222

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:29,952,708..29,952,708 [-]

(Levis, 2001.8.30, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal pain response | early third instar larval stage | conditional (with Df(3R)Exel6213)

(Im et al., 2015)

abnormal pain response | early third instar larval stage | conditional (with TkR99D^MB09356)

(Im et al., 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

24 hours after UV-induced nociceptive sensitization, early third instar TkR99D^s2222/Df(3R)Exel6213 or TkR99D^MB09356/TkR99D^s2222 larvae show significant reduction of thermal allodynia (withdrawal responses to a sub-threshold 38[o]C probe) compared to wild type or heterozygote larvae.

(Im et al., 2015)

FlyBase curator comment: Stated to be recessive lethal, however see Cook, 2006.1.31, personal communication to FlyBase for evidence that lethality does not map to insertion.

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

12161

w¹¹¹⁸; P{lacW}TkR99D^s2222/TM3, Sb¹

Kyoto

111424

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}82B P{lacW}TkR99D^s2222/TM6B, P{Car20y}TPN1, Tb¹

Notes on Origin

Discoverer

M. Scott.

Comments

P{lacW}Takr99D^s2222 complements Df(3R)X3F showing that the homozygous lethality of the P{lacW}Takr99D^s2222 chromosome is not due to the insertion itself.

(Cook, 2006.1.31)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Takr99D^s2222

TkR99D^s2222

dtkr^s2222

(Im et al., 2015)

l(3)s2222

(Spradling et al., 1999)

l(3)s2222^s2222

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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