FlyBase Gene Report: Dmel\mth

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Gene: Dmel\mth

General Information

Symbol

Dmel\mth

Species

D. melanogaster

Name

methuselah

Annotation Symbol

CG6936

Feature Type

protein_coding_gene

FlyBase ID

FBgn0023000

Gene Model Status

Stock Availability

14 publicly available

Gene Summary

methuselah (mth) encodes a member of the Class B secretin family of G-protein-coupled receptors involved in lifespan. mth product also plays a role in the processes of learning and memory formation via the CREB pathway. [Date last reviewed: 2018-09-20] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

Sequence location

3L:343,378..346,957 [-]

Recombination map

3-0.5

RefSeq locus

NT_037436 REGION:343378..346957

Sequence

Get Decorated FASTA

Genomic Maps

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (15 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

enables G protein-coupled peptide receptor activity

inferred from physical interaction with FLYBASE:sun; FB:FBgn0014391

(Cvejic et al., 2004)

enables G protein-coupled receptor activity

inferred from direct assay

(Ja et al., 2007)

enables peptide binding

inferred from physical interaction with FLYBASE:sun; FB:FBgn0014391

(Cvejic et al., 2004)

enables protein binding

inferred from physical interaction with UniProtKB:Q9VXN2

(Cvejic et al., 2004)

inferred from physical interaction with UniProtKB:A0A1F4

(Ja et al., 2009)

inferred from physical interaction with UniProtKB:P05623

(Ja et al., 2009)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables G protein-coupled peptide receptor activity

inferred from biological aspect of ancestor with PANTHER:PTN002932399

(GO Reference Genome Project, 2011-)

enables G protein-coupled receptor activity

traceable author statement

(Brody and Cravchik, 2000)

inferred from electronic annotation with InterPro:IPR000832, InterPro:IPR010596, InterPro:IPR036272

(InterPro Project Members, 2004-)

enables transmembrane signaling receptor activity

inferred from electronic annotation with InterPro:IPR017981

(InterPro Project Members, 2004-)

Biological Process (8 terms)

Terms Based on Experimental Evidence (7 terms)

CV Term

Evidence

References

involved_in determination of adult lifespan

inferred from direct assay

(Ja et al., 2007)

inferred from mutant phenotype

(Petrosyan et al., 2014)

inferred from mutant phenotype

(Schmidt et al., 2000, Lin et al., 1998)

involved_in G protein-coupled receptor signaling pathway

inferred from direct assay

(Ja et al., 2007, Cvejic et al., 2004)

involved_in response to heat

inferred from mutant phenotype

(Lin et al., 1998)

involved_in response to paraquat

inferred from mutant phenotype

(Shukla et al., 2014)

involved_in response to reactive oxygen species

inferred from mutant phenotype

(Lin et al., 1998)

involved_in response to starvation

inferred from mutant phenotype

(Lin et al., 1998)

involved_in synaptic vesicle exocytosis

inferred from mutant phenotype

(Song et al., 2002)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in cell surface receptor signaling pathway

inferred from electronic annotation with InterPro:IPR017981

(InterPro Project Members, 2004-)

involved_in G protein-coupled receptor signaling pathway

inferred from biological aspect of ancestor with PANTHER:PTN002932399

(GO Reference Genome Project, 2011-)

traceable author statement

(Brody and Cravchik, 2000)

inferred from electronic annotation with InterPro:IPR000832

(InterPro Project Members, 2004-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in plasma membrane

inferred from direct assay

(Cvejic et al., 2004)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in membrane

inferred from electronic annotation with InterPro:IPR000832, InterPro:IPR017981

(InterPro Project Members, 2004-)

traceable author statement

(Brody and Cravchik, 2000)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN002932399

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

METHUSELAH-TYPE RECEPTORS

Protein Family (UniProt)

Belongs to the G-protein coupled receptor 2 family. Mth subfamily. (O97148)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

methuselah (mth) encodes a member of the Class B secretin family of G-protein-coupled receptors involved in lifespan. mth product also plays a role in the processes of learning and memory formation via the CREB pathway. [Date last reviewed: 2018-09-20]

Gene Group (FlyBase)

METHUSELAH-TYPE RECEPTORS -: Methuselah GPCRs are Class B GPCRs, forming the B3 subgroup. The gene family's founding member methuselah (mth) was identified as a Drosophila GPCR involved in the modulation of life span and stress response. No counterpart for the methuselah gene family have been identified in vertebrates. (Adapted from FBrf0129744 and FBrf0218628).

Protein Function (UniProtKB)

Involved in biological aging and stress response. Essential for adult survival. Required in the presynaptic motor neuron to up-regulate neurotransmitter exocytosis at larval glutamatergic neuromuscular junctions (NMJs). Regulates a step associated with docking and clustering of vesicles at release sites. SP/Acp70A and sun are agonists that activate mth in vitro.

(UniProt, O97148)

Summary (Interactive Fly)

G-protein coupled receptor: mutants have increased longevity, are resistant to stress and have increased body weight - Drosophila insulin release is triggered by the adipose Stunted ligand of the brain Methuselah receptor

Interactive Fly

Gene Model and Products

Number of Transcripts

3

Number of Unique Polypeptides

2

Please see the JBrowse view of Dmel\mth for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry O97148)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

1FJR
2PZX

Comments on Gene Model

Gene model reviewed during 5.40

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0072570

1967

514

FBtr0072571

1882

522

FBtr0306850

2420

514

Additional Transcript Data and Comments

Reported size (kB)

1.948 (longest cDNA)

(Lin et al., 1998)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

FBpp0072469

59.6

514

6.98

FBpp0072470

60.4

522

8.59

FBpp0297746

59.6

514

6.98

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

514 aa isoforms: mth-PA, mth-PE

Additional Polypeptide Data and Comments

Reported size (kDa)

514 (aa)

(Lin et al., 1998)

Comments

External Data

Subunit Structure (UniProtKB)

Homodimer.

(UniProt, O97148)

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

1FJR
2PZX

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mth using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

mth_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.65

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism | ubiquitous

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 8

cephalic furrow

(Patel et al., 2012)

amnioproctodeal invagination

(Patel et al., 2012)

(Patel et al., 2012)

embryonic stage 9 -- 10

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 16

ventral midline of embryo

(Patel et al., 2012)

embryonic/larval pharynx

(Patel et al., 2012)

embryonic stage 13

(Patel et al., 2012)

embryonic/larval dorsal pouch

(Patel et al., 2012)

embryonic stage 13 -- 16

organism | faint | ubiquitous

(Fisher et al., 2012)

third instar larval stage

eye disc morphogenetic furrow

(Patel et al., 2012)

eye disc | posterior

(Patel et al., 2012)

(Patel et al., 2012)

(Patel et al., 2012)

Additional Descriptive Data

mth expression is observed at stage 8 in the cephalic furrow, proctodeum, and ventral mesoderm. At stage 13, expression is seen in the ventral midline, pharynx, and dorsal pouch. In third instar larvae, expression is observed in the neuropil, at the morphogenetic furrow and in wing and leg discs.

(Patel et al., 2012)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in plasma membrane

inferred from direct assay

(Cvejic et al., 2004)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\mth in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\mth

View exonic expression by tissue for Dmel\mth

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0023000

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38058/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0023000

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0023000

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0023000

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6936

Flygut - An atlas of the Drosophila adult midgut

FBgn0023000

Images

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 13 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 11 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of mth

Transgenic constructs containing regulatory region of mth

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene partially disrupted in

(Rao and Deng, 2019.10.23)

Inferred from location ( 8 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die during embryonic stage | recessive

mth^Δ

mth¹

viable (with mth¹)

mth^Δ

viable (with mth^Δ)

mth¹

Sterility

Allele

mth¹

Other Phenotypes

Allele

abnormal neuroanatomy

mth¹

abnormal neurophysiology

mth¹

abnormal neurophysiology, with Scer\GAL4^hs.PB

mth^UAS.cSa

abnormal neurophysiology, with Scer\GAL4^Toll-6-D42

mth^UAS.cSa

abnormal neurophysiology (with mth¹)

mth^Δ6

abnormal neurophysiology (with mth^Δ6)

mth¹

abnormal oxidative stress response

mth¹

abnormal oxidative stress response, with Scer\GAL4^ple.PF

abnormal sleep | adult stage

mth^attP

abnormal stress response

mth¹

chemical resistant

mth¹

chemical resistant, with Scer\GAL4^ple.PF

mth^KK106399

chemical resistant | recessive

mth¹

chemical sensitive, with Scer\GAL4^ple.PF

mth^UAS.GFP

decreased cell number | spermatogenesis

mth^Ex28

increased occurrence of cell division | progressive | spermatogenesis

mth¹

increased size | adult stage, with Scer\GAL4^nub.PU

mth^KK106399

long lived | recessive

mth¹

some die during embryonic stage | recessive

mth^Δ

visible | adult stage, with Scer\GAL4^nub.PU

mth^KK106399

mth^R3

Phenotype manifest in

Allele

mth^Ex28

male germline stem cell

mth¹

mth¹

synaptic vesicle

mth¹

wing, with Scer\GAL4^nub.PU

mth^KK106399

wing vein | ectopic, with Scer\GAL4^nub.PU

mth^KK106399

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (35)

2 of 14

Yes

Yes

2 of 14

Yes

No

2 of 14

Yes

No

2 of 14

Yes

No

2 of 14

Yes

No

2 of 14

Yes

No

2 of 14

Yes

Yes

2 of 14

Yes

Yes

2 of 14

Yes

Yes

2 of 14

Yes

No

2 of 14

Yes

No

2 of 14

Yes

No

2 of 14

Yes

No

2 of 14

Yes

No

2 of 14

Yes

No

2 of 14

Yes

No

2 of 14

Yes

No

2 of 14

Yes

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1

1 of 14

No

Yes

1 of 14

No

No

1 of 14

No

No

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (25)

2 of 14

Yes

Yes

2 of 14

Yes

No

2 of 14

Yes

No

2 of 14

Yes

Yes

2 of 14

Yes

Yes

2 of 14

Yes

Yes

2 of 14

Yes

No

2 of 14

Yes

No

2 of 14

Yes

No

2 of 14

Yes

No

2 of 14

Yes

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

Yes

1 of 14

No

Yes

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

Yes

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

Mus musculus (laboratory mouse) (28)

2 of 14

Yes

Yes

2 of 14

Yes

No

2 of 14

Yes

Yes

2 of 14

Yes

Yes

2 of 14

Yes

Yes

2 of 14

Yes

Yes

2 of 14

Yes

Yes

2 of 14

Yes

Yes

2 of 14

Yes

No

2 of 14

Yes

No

2 of 14

Yes

No

2 of 14

Yes

No

2 of 14

Yes

No

2 of 14

Yes

No

2 of 14

Yes

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

Xenopus tropicalis (Western clawed frog) (8)

2 of 13

Yes

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

Yes

1 of 13

No

Yes

Danio rerio (Zebrafish) (46)

3 of 14

Yes

Yes

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

Yes

2 of 14

No

Yes

2 of 14

No

No

2 of 14

No

Yes

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

1 of 14

No

No

1 of 14

No

Yes

1 of 14

No

Yes

1 of 14

No

Yes

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

Yes

1 of 14

No

Yes

1 of 14

No

Yes

1 of 14

No

Yes

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

Drer\LOC100331278

1 of 14

No

No

Drer\LOC100536657

1 of 14

No

Yes

Drer\LOC100538283

1 of 14

No

No

Drer\LOC103909389

1 of 14

No

Yes

Drer\LOC103909395

1 of 14

No

No

Drer\LOC103911493

1 of 14

No

Yes

Drer\LOC108181087

1 of 14

No

Yes

Drer\LOC108182851

1 of 14

No

Yes

Drer\LOC110439896

1 of 14

No

Yes

Drer\LOC110439897

1 of 14

No

Yes

Caenorhabditis elegans (Nematode, roundworm) (3)

1 of 14

Yes

No

1 of 14

Yes

No

1 of 14

Yes

No

Anopheles gambiae (African malaria mosquito) (11)

6 of 12

Yes

Yes

5 of 12

No

No

5 of 12

No

No

3 of 12

No

No

Agam\AgaP_AGAP000130

2 of 12

No

No

2 of 12

No

No

2 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

Arabidopsis thaliana (thale-cress) (1)

1 of 13

Yes

No

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:mth. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (22)

10 of 13

9 of 13

9 of 13

9 of 13

8 of 13

8 of 13

8 of 13

7 of 13

7 of 13

6 of 13

5 of 13

4 of 13

4 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

model of alcohol-related neurodevelopmental disorder

CEA

(Belhorma et al., 2021)

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

phenotype-based detection assay, peptide massfingerprinting

(Cvejic et al., 2004)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

Homodimer.

(UniProt, O97148 )

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0023000

MIST (protein-protein) - An integrated Molecular Interaction Database

38058

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

3L

Recombination map

3-0.5

Cytogenetic map

Sequence location

3L:343,378..346,957 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

61C1-61C1

Limits computationally determined from genome sequence between P{PZ}trh¹⁰⁵¹² and P{EP}EP3104^EP3104

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

61C-61C

(Lin, 1998.11.24)

Experimentally Determined Recombination Data

Location

3-0.5

(FlyBase, 2016)

3-0.1

(Comeron et al., 2012)

3-

(Lin et al., 1998)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (14)

Aberrations including deletions of this gene Aberrations including duplications of this gene

64194

w^*; P{UAS-mth.S}M-45

84655

TI{2A-GAL4}mth^2A-B.GAL4

27896

w^*; P{lacW}mth¹

97811

w¹¹¹⁸; P{UAS-mth.gRNAx3.pCFD6}attP1

27495

y¹ v¹; P{TRiP.JF02645}attP2

27904

w^*; mth^Δ6/TM6B, Tb¹

84654

TI{2A-GAL4}mth^2A-AE.GAL4/TM6B, Tb¹

84526

w^*; Df(3L)mth^attP, TI{TI}mth^attP

24837

w¹¹¹⁸; Mi{ET1}mth^MB04912 Ptpmeg^MB04912

36823

y¹ sc^* v¹ sev²¹; P{TRiP.GL01055}attP2

v330139

P{VSH330139}attP40

v102303

P{KK106399}VIE-260B

More stocks available...

Genomic Clones (17)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (32)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

FI09612

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0023000

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0023000

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

mth is required in the presynaptic motor neuron to acutely upregulate neurotransmitter exocytosis at larval neuromuscular junctions.

(Song et al., 2002)

Defined during a screen for extended lifespan.

(Lin and Benzer, 1997)

mth mutants show a greater than 50% increase in lifespan. Paraquat resistance is also increased.

(Lin and Benzer, 1997)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

CG6936

(Mao et al., 2024, Meadows, 2017.1.11, Friedrich and Jones, 2016, Jayakumar et al., 2016, Li et al., 2013, Chen et al., 2012, Ni et al., 2011.7.19, Zhang et al., 2010, Keleman et al., 2009.8.5, Perkins et al., 2009, Schiess et al., 2009, Wittkopp et al., 2008, Laviolette et al., 2005, Harmar, 2001, Mount, 2001.8.14)

MTH

(Chen et al., 2024, Lotfy et al., 2024, Zhu et al., 2024, Oxenkrug and Navrotska, 2023, Guo et al., 2022, Han et al., 2021, Yue et al., 2021, Wang et al., 2015, Peng et al., 2012, Peng et al., 2009, Longo, 1999)

Mth

(Wang et al., 2024, Himalian et al., 2022, Ratnaparkhi and Sudhakaran, 2022, Meschi and Delanoue, 2021, Gáliková and Klepsatel, 2018, Staats et al., 2018, Sheng et al., 2017, Sung et al., 2017, Zuo et al., 2013, Cui et al., 2012, Zuo et al., 2012, Heo et al., 2008, Ja et al., 2007, Landry et al., 2005, Longo, 1999)

mth

(Bischoff et al., 2025, Titus et al., 2023, Promislow et al., 2022, Schlichting et al., 2022, Liu et al., 2021, Yang et al., 2021, Yi et al., 2021, Delanoue and Romero, 2020, Flatt, 2020, Weigelt et al., 2020, Evangelakou et al., 2019, Huang et al., 2019, Krittika and Yadav, 2019, Rao and Deng, 2019.10.23, Tang et al., 2019, Wang et al., 2019, Schmähling et al., 2018, Zhang et al., 2018, Bayer et al., 2017, Jo and Imm, 2017, Friedrich and Jones, 2016, Pandey et al., 2015, Burke et al., 2014, Petrosyan et al., 2014, Shukla et al., 2014, Araújo et al., 2013, Gimenez et al., 2013, Sen et al., 2013, Sgrò et al., 2013, Webber et al., 2013, Pezzulo et al., 2012, Sharma et al., 2011, Lee et al., 2010, Dickman and Davis, 2009, Ja et al., 2009, Paaby and Schmidt, 2008, Suh et al., 2008, Wallenfang et al., 2008, Wittkopp et al., 2008, Laayouni et al., 2007, Martinez et al., 2007, Paaby and Schmidt, 2007, Petrosyan et al., 2007, Dierick and Greenspan, 2006, Kim et al., 2006, Wallenfang et al., 2006, Laviolette et al., 2005, Schmidt et al., 2005, Williams and Thompson, 2005, Aravamudan and Broadie, 2003, Duvernell et al., 2003)

Name Synonyms

Methuselah

(Saad and Hipfner, 2021, Texada et al., 2020, Lees et al., 2016, Petrosyan et al., 2014, Gimenez et al., 2013, Cui et al., 2012, Dong et al., 2012, Eleftherianos and Castillo, 2012, Heo et al., 2008, Ja et al., 2007, Petrosyan et al., 2007, Cho et al., 2005, Williams and Thompson, 2005)

methusalah

(Wallenfang et al., 2008)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 112 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

38058

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

O97148

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

O97148

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

E1JHX3

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

O97148

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0023000

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38058/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0023000

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0023000

FlyMine - An integrated database for Drosophila genomics

FBgn0023000

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG6936

MARRVEL_MODEL - MARRVEL (model organism gene)

38058

PDB - An information portal to biological macromolecular structures

1FJR
2PZX

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0023000

DroID - A comprehensive database of gene and protein interactions.

FBgn0023000

DRSC - Results frm RNAi screens

FBgn0023000

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

mth_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0023000

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0023000

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6936

Flygut - An atlas of the Drosophila adult midgut

FBgn0023000

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC030964

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

38058

References (183)