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General Information
Symbol
P{ple-GAL4.F}
FlyBase ID
FBtp0114847
Feature type
transgenic_transposable_element
Component Allele(s)
Also Known As
TH-GAL4, ple-GAL4, THGAL4, (TH)-Gal4, pleGAL4, TH Gal4, TH, Tyrosine Hydroxylase (TH)-GAL4, P{TH-GAL4}
Size
Expression Data
GAL4 reporter/driver
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
Construct components
Product class / Tool use(s)
Marker(s)
Component allele Scer\GAL4ple.PF
Product class / Tool use(s)
Regulatory region(s)
Description

A ple genomic fragment from -4168 to +136bp relative to the transcription start site is fused upstream of sequence encoding a GAL4 driver plus a 3' translation termination element from the Hsp70 gene. The ple coding sequence (from +183 to +6820 relative to the transcription start site) is fused downstream of the Hsp70 translation termination sequence (so that any regulatory sequences present in the coding region are included, but the ple coding sequence is not translated).

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
binary
component
selectable
marker
Component Alleles
Reference(s)
(Aguilar et al., 2021, Aqsa and Sarkar, 2021, Bhat et al., 2021, Bridi et al., 2021, Buck et al., 2021, Carvajal-Oliveros et al., 2021, Gumeni et al., 2021, Ham et al., 2021, Landayan et al., 2021, Lund et al., 2021, Melnattur et al., 2021, Sareen et al., 2021, Xie et al., 2021, Blosser et al., 2020, Dabool et al., 2020, Davla et al., 2020, Delventhal and Steinhauer, 2020, Fernandez-Chiappe et al., 2020, Fernández-Cruz et al., 2020, Golomidov et al., 2020, Ham et al., 2020, King et al., 2020, Klein et al., 2020, Kondo et al., 2020, Luan et al., 2020, Park et al., 2020, Petridi et al., 2020, Pirooznia et al., 2020, Pradhan et al., 2020, Rallis et al., 2020, Robles-Murguia et al., 2020, Saikumar et al., 2020, Sharma and Hasan, 2020, Shimizu et al., 2020, Siima et al., 2020, Sun et al., 2020, Wang et al., 2020, Wiggin et al., 2020, Williams et al., 2020, Yalgin et al., 2020, Zhao and Zhou, 2020, Agrawal et al., 2019, Babski et al., 2019, Boto et al., 2019, Campbell et al., 2019, Chen et al., 2019, Deng et al., 2019, Gao et al., 2019, Hajji et al., 2019, Handler et al., 2019, Hill et al., 2019, Maitra et al., 2019, Man Anh et al., 2019, Massey et al., 2019, Meissner et al., 2019, Ni et al., 2019, Ortega-Arellano et al., 2019, Phan et al., 2019, Sapiro et al., 2019, Schaffner et al., 2019, Shukla et al., 2019, Trostnikov et al., 2019, Truong et al., 2019, Wu et al., 2019, Zhang et al., 2019, Zhou et al., 2019, Zhou et al., 2019, Cackovic et al., 2018, Cornelissen et al., 2018, Cunningham et al., 2018, Ferris et al., 2018, Issa et al., 2018, Jois et al., 2018, Landayan et al., 2018, Lee et al., 2018, Lee et al., 2018, Martinez-Perez et al., 2018, Nguyen et al., 2018, Omoto et al., 2018, Ordonez et al., 2018, Pu et al., 2018, Pu et al., 2018, Ravi et al., 2018, Sonn et al., 2018, Steinkellner et al., 2018, Sun et al., 2018, Tran et al., 2018, Valadas et al., 2018, Xie et al., 2018, Xu and Xu, 2018, Aguilar et al., 2017, Branch et al., 2017, Cervantes-Sandoval et al., 2017, Chng et al., 2017, Cichewicz et al., 2017, Cording et al., 2017, Edenharter et al., 2017, Hartenstein et al., 2017, Hosaka et al., 2017, Inoshita et al., 2017, Jang et al., 2017, Kim et al., 2017, Kim et al., 2017, Liu et al., 2017, Niens et al., 2017, Pathak et al., 2017, Qian et al., 2017, Quintero-Espinosa et al., 2017, Søvik et al., 2017, Ueno et al., 2017, Xie et al., 2017, Yang et al., 2017, Cervantes-Sandoval et al., 2016, Clark et al., 2016, Davis et al., 2016, Drago and Davis, 2016, Filograna et al., 2016, Freyberg et al., 2016, Grillet et al., 2016, Guven-Ozkan et al., 2016, Ma et al., 2016, Pimentel et al., 2016, Ren et al., 2016, Scholz-Kornehl and Schwärzel, 2016, Sen et al., 2016, Wang et al., 2016, Williams et al., 2016, Xu et al., 2016, Yilmazer et al., 2016, Zhu et al., 2016, Agrawal and Hasan, 2015, Berry et al., 2015, Breda et al., 2015, Cartier et al., 2015, Cassar et al., 2015, Cohn et al., 2015, Gehrke et al., 2015, Hartenstein et al., 2015, Jeong et al., 2015, Kirkhart and Scott, 2015, Krüttner et al., 2015, Linford et al., 2015, Lovick and Hartenstein, 2015, Mortiboys et al., 2015, Okamoto and Nishimura, 2015, Pathak et al., 2015, Poças et al., 2015, Sadaf et al., 2015, Shankar et al., 2015, Shih et al., 2015, Srivastav et al., 2015, Stratoulias and Heino, 2015, Stratoulias and Heino, 2015, Tschida and Bhandawat, 2015, Wiemerslage and Lee, 2015, Zhang et al., 2015, Afsari et al., 2014, Bath et al., 2014, Bingol et al., 2014, Bjordal et al., 2014, Boto et al., 2014, Choksi et al., 2014, Dawydow et al., 2014, Galili et al., 2014, Hamilton et al., 2014, Hwang et al., 2014, Inamdar et al., 2014, Klein et al., 2014, Knight et al., 2014, Kunst et al., 2014, Lin et al., 2014, Mukherjee et al., 2014, Navarro et al., 2014, Oh et al., 2014, Roy and Jackson, 2014, Schinaman et al., 2014, Schoofs et al., 2014, Shiraishi et al., 2014, Shukla et al., 2014, Shukla et al., 2014, Slawson et al., 2014, Varga et al., 2014, Vogt et al., 2014, von Philipsborn et al., 2014, Yin et al., 2014, Yuan et al., 2014, Yun et al., 2014, Alekseyenko et al., 2013, Bader et al., 2013, Calcagno et al., 2013, Esposito et al., 2013, Hamilton et al., 2013, Hartl et al., 2013, Hindle et al., 2013, Kim and Yim, 2013, Ortega-Arellano et al., 2013, Pech et al., 2013, Pech et al., 2013, Riemensperger et al., 2013, Wiemerslage et al., 2013, Wu et al., 2013, Wu et al., 2013, Zhu et al., 2013, Aso et al., 2012, Chen et al., 2012, Chen et al., 2012, Duran et al., 2012, Fouillet et al., 2012, Freeman et al., 2012, Gonzalo-Gomez et al., 2012, Humphrey et al., 2012, Inagaki et al., 2012, Ishida et al., 2012, Islam et al., 2012, Kahsai et al., 2012, Kallijärvi et al., 2012, Keleman et al., 2012, Kim et al., 2012, Koh et al., 2012, Liu et al., 2012, Liu et al., 2012, Liu et al., 2012, Mandilaras and Missirlis, 2012, Marella et al., 2012, Munoz-Soriano et al., 2012, Neckameyer and Bhatt, 2012, Plaçais et al., 2012, Schneider et al., 2012, Xiong et al., 2012, Bang et al., 2011, Barone et al., 2011, Chiang et al., 2011, Demilly et al., 2011, Gajula Balija et al., 2011, Giang et al., 2011, Hernández-Vargas et al., 2011, Jepson et al., 2011, Kaun et al., 2011, Kolodziejczyk and Nässel, 2011, Lee et al., 2011, Riemensperger et al., 2011, Rival et al., 2011, Stavropoulos and Young, 2011, Tio et al., 2011, Vickrey and Venton, 2011, Wakabayashi-Ito et al., 2011, Wang et al., 2011, Alekseyenko et al., 2010, Aso et al., 2010, Bayersdorfer et al., 2010, Daubert et al., 2010, de Haro et al., 2010, Du et al., 2010, Gehrke et al., 2010, Imai et al., 2010, Kim et al., 2010, Kong et al., 2010, Liu and Lu, 2010, Makos et al., 2010, Melicharek et al., 2010, Pauls et al., 2010, Pauls et al., 2010, White et al., 2010, Wu et al., 2010, Yarali and Gerber, 2010, Yu et al., 2010, Al-Anzi et al., 2009, Claridge-Chang et al., 2009, Corl et al., 2009, Gruenewald et al., 2009, Honjo and Furukubo-Tokunaga, 2009, Jansen et al., 2009, Mao and Davis, 2009, Palgi et al., 2009, Park et al., 2009, Sakai et al., 2009, Selcho et al., 2009, Tain et al., 2009, Venderova et al., 2009, Botella et al., 2008, Gao et al., 2008, Imai et al., 2008, Johard et al., 2008, Kaplan et al., 2008, Lee et al., 2008, Lee et al., 2008, Lee et al., 2008, Nässel et al., 2008, Sitaraman et al., 2008, Tanaka et al., 2008, Trinh et al., 2008, Vömel and Wegener, 2008, Yang et al., 2008, Yang et al., 2008, Dierick and Greenspan, 2007, Draper et al., 2007, Lavara-Culebras and Paricio, 2007, Lee et al., 2007, Liu et al., 2007, Muñoz-Soriano and Paricio, 2007, Sang et al., 2007, Zhang et al., 2007, Zhang et al., 2007, Cooper et al., 2006, Ganguly-Fitzgerald et al., 2006, Park et al., 2006, Park et al., 2006, Schroll et al., 2006, Wheeler et al., 2006, Yang et al., 2006, Yuan et al., 2006, Andretic et al., 2005, Auluck et al., 2005, Lima and Miesenbock, 2005, Melcher and Pankratz, 2005, Menzies et al., 2005, Riemensperger et al., 2005, Sykes and Condron, 2005, Whitworth et al., 2005, Yang, 2005, Yang et al., 2005, Friggi-Grelin et al., 2003, Friggi-Grelin et al., 2003, Schroeder et al., 2003, Schwaerzel et al., 2003)
Molecular data

A ple genomic fragment from -4168 to +136bp relative to the transcription start site is fused upstream of sequence encoding a GAL4 driver plus a 3' translation termination element from the Hsp70 gene. The ple coding sequence (from +183 to +6820 relative to the transcription start site) is fused downstream of the Hsp70 translation termination sequence (so that any regulatory sequences present in the coding region are included, but the ple coding sequence is not translated).

Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
ellipsoid body

Comment: medium expression

eye

Comment: faint expression

Additional Information
Statement
Reference

Drives expression in dopaminergic neurons.

Expression driven by Scer\GAL4ple.PF was restricted via a transgenic cell class-lineage intersection (CLIn) system to a subset of neuronal clusters - PPM3 and a subset of PPM2 and PPL1 neurons - of specific Type II neuroblast lineages using Klac\KDRstg14. All 8 PPM3 neurons are part of the DM6 neuroblast lineage, two of these, originating from the first larval born secondary neuroblast, are EB neurons, while the mFB and vFB neurons originate from other secondary neuroblasts. The subset of PPM2 neurons are part of the DM2 (0-1 neurons), DM3 (0-1 neurons) and DM4 (1 neuron) neuroblast lineages. The 2 dFB neurons of the dopaminergic PPL1 cluster are part of the DL1 neuroblast lineage, while the remaining 5 non-dFB neurons are part of the DL2 neuroblast lineage. These 5 neurons include a MB-alpha neuron of the dopaminergic PPL1 cluster, a MB-alpha' neuron of the dopaminergic PPL1 cluster, a mushroom body vertical lobe arborizing neuron 1 and a further neuron that targets the superior medial protocerebrum and superior intermediate protocerebrum.

Scer\GAL4ple.PF drives expression in 1-3 SP1 neurons, 1-4 SP2 neurons, 3-6 IP neurons, all LP1 neurons, 2-4 LP2 neurons, 1-3 SE1 neurons, 1-3 adult serotonergic LP3 neurons and 1 adult serotonergic AP neuron. Additionally, it also drives expression in small numbers (1-14) of non-serotonergic neurons adjacent to SP1, SP2, IP, LP1, LP2, LP3, SE1, SE3, T1, T2, T3, and abdominal serotonergic neurons.

Scer\GAL4ple.PF drives expression in all dopaminergic neurons of the PAL PPM1/2, PPL1. PPM3 and PPL2 clusters which project to the vertical lobes of the mushroom body, the ellipsoid and the fan-shaped body. Expression is only found in around 10% of dopaminergic neurons of the PAM cluster.

Scer\GAL4ple.PF drives expression in the dopaminergic PPL1 neurons (includes MB-MP1, MB-V1 and MB-MV1 neurons), PPL2ab, in 118 PAM neurons per hemisphere (includes MB-M3 neurons), in PPM3, in 1 PPM1 neuron per hemisphere, in PPM2, PPL2c, VUM1 and VUM2 neurons. Expression of Scer\GAL4ple.PF is also detected in dopaminergic T1, SVP1, D1 and SP1 neurons.

Scer\GAL4ple.PF drives expression in a subset of the dopaminergic neurons in the adult brain labelled by ple : around 8 PAM neurons, 5 PAL neurons, 4 PPM1 neuron, 8 PPM2 neurons, 7 PPM3 neurons, 11 PPL1 neurons and 6 PPL2 neurons.

Expression observed in neurons that innervate the mushroom body vertical lobes and peduncles, the fan-shaped body and the protocerebral bridge.

Scer\GAL4ple.PF is expressed in approximately 12 PPL1, 11 PPL2, 8 PPM1/2, 7 PPM3 and 5 PAL dopaminergic neurons, but is not expressed well in the PAM cluster. PAL neurons are seen to project across the midline. Neurons of the PPM1/2 cluster are seen to project ventrally. PPL1 cluster neurons are seen to project medially and dorsally towards the mushroom body, and the PPM3 neurons are observed to project towards the central complex.

Approximately 127 Scer\GAL4ple.PF expressing cells can be identified in the adult protocerebrum. Scer\GAL4ple.PF is highly expressed in neurons that terminate in the vertical lobes of the mushroom body and in the lateral part of the mushroom body horizontal lobes. There are also a few fibers wrapped around the distal tip of the mushroom body horizontal lobes.

Scer\GAL4ple.PF drives expression in the dopaminergic cluster PAL, PPL1, PPL2ab, PPM1, 10 neurons of the PPM2 cluster, 8 neurons of the PPM3 cluster and 13 neurons of the PAM cluster.

Scer\GAL4ple.PF generally labels all dopaminergic neurons.

Scer\GAL4ple.PF labelled neurons that innervated, particularly, the , midregion of the peduncle, the heel, alpha lobe and lateral region of the gamma lobe. Labelled processes ate also found surrounding the median portion of the beta and gamma lobes. Expression in larva is similar to adults, with Scer\GAL4ple.PF driving expression in neurons that innervate the the vertical lobes and heel.

 
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (46)
Reported As
Symbol Synonym
Pple(TH)-Gal4
P{ple-GAL4.G}
P{w+mC=ple-GAL4.F}
TH (tyrosine hydroxylase)-GAL4
TH-GAL4
(Aguilar et al., 2021, Buck et al., 2021, Melnattur et al., 2021, Sareen et al., 2021, Kondo et al., 2020, Petridi et al., 2020, Saikumar et al., 2020, Shimizu et al., 2020, Siima et al., 2020, Yalgin et al., 2020, Zhao and Zhou, 2020, Agrawal et al., 2019, Gao et al., 2019, Hajji et al., 2019, Man Anh et al., 2019, Meissner et al., 2019, Ortega-Arellano et al., 2019, Phan et al., 2019, Shukla et al., 2019, Truong et al., 2019, Cackovic et al., 2018, Cornelissen et al., 2018, Jois et al., 2018, Lee et al., 2018, Nguyen et al., 2018, Ordonez et al., 2018, Pu et al., 2018, Pu et al., 2018, Steinkellner et al., 2018, Tran et al., 2018, Xie et al., 2018, Aguilar et al., 2017, Branch et al., 2017, Cording et al., 2017, Edenharter et al., 2017, Hosaka et al., 2017, Kim et al., 2017, Qian et al., 2017, Søvik et al., 2017, Ueno et al., 2017, Xie et al., 2017, Yang et al., 2017, Davis et al., 2016, Drago and Davis, 2016, Filograna et al., 2016, Freyberg et al., 2016, Grillet et al., 2016, Guven-Ozkan et al., 2016, Pimentel et al., 2016, Ren et al., 2016, Yilmazer et al., 2016, Cartier et al., 2015, Cassar et al., 2015, Poças et al., 2015, Shih et al., 2015, Tschida and Bhandawat, 2015, Wiemerslage and Lee, 2015, Zhang et al., 2015, Afsari et al., 2014, Bath et al., 2014, Bjordal et al., 2014, Boto et al., 2014, Choksi et al., 2014, Galili et al., 2014, Inamdar et al., 2014, Klein et al., 2014, Knight et al., 2014, Kunst et al., 2014, Lin et al., 2014, Mukherjee et al., 2014, Navarro et al., 2014, Roy and Jackson, 2014, Varga et al., 2014, Vogt et al., 2014, von Philipsborn et al., 2014, Yun et al., 2014, Bader et al., 2013, Hamilton et al., 2013, Wu et al., 2013, Zhu et al., 2013, Aso et al., 2012, Chen et al., 2012, Freeman et al., 2012, Ishida et al., 2012, Kahsai et al., 2012, Keleman et al., 2012, Koh et al., 2012, Liu et al., 2012, Liu et al., 2012, Munoz-Soriano et al., 2012, Schneider et al., 2012, Xiong et al., 2012, Barone et al., 2011, Chiang et al., 2011, Giang et al., 2011, Kaun et al., 2011, Riemensperger et al., 2011, Rival et al., 2011, Tio et al., 2011, Wakabayashi-Ito et al., 2011, Aso et al., 2010, de Haro et al., 2010, Du et al., 2010, Imai et al., 2010, Kong et al., 2010, Makos et al., 2010, Pauls et al., 2010, Pauls et al., 2010, Yu et al., 2010, Claridge-Chang et al., 2009, Corl et al., 2009, Honjo and Furukubo-Tokunaga, 2009, Jansen et al., 2009, Mao and Davis, 2009, Palgi et al., 2009, Park et al., 2009, Selcho et al., 2009, Botella et al., 2008, Lee et al., 2008, Sitaraman et al., 2008, Tanaka et al., 2008, Trinh et al., 2008, Yang et al., 2008, Yang et al., 2008, Lavara-Culebras and Paricio, 2007, Lee et al., 2007, Muñoz-Soriano and Paricio, 2007, Sang et al., 2007, Cooper et al., 2006, Park et al., 2006, Yang et al., 2006, Auluck et al., 2005, Lima and Miesenbock, 2005, Menzies et al., 2005, Sykes and Condron, 2005, Yang, 2005, Yang et al., 2005, Friggi-Grelin et al., 2003, Friggi-Grelin et al., 2003)
TH-Gal4
(Aqsa and Sarkar, 2021, Bhat et al., 2021, Landayan et al., 2021, Blosser et al., 2020, Davla et al., 2020, Fernandez-Chiappe et al., 2020, Fernández-Cruz et al., 2020, King et al., 2020, Luan et al., 2020, Park et al., 2020, Pirooznia et al., 2020, Pradhan et al., 2020, Rallis et al., 2020, Robles-Murguia et al., 2020, Sun et al., 2020, Wiggin et al., 2020, Babski et al., 2019, Boto et al., 2019, Campbell et al., 2019, Deng et al., 2019, Handler et al., 2019, Maitra et al., 2019, Ni et al., 2019, Sapiro et al., 2019, Wu et al., 2019, Zhang et al., 2019, Zhou et al., 2019, Cunningham et al., 2018, Issa et al., 2018, Landayan et al., 2018, Lee et al., 2018, Martinez-Perez et al., 2018, Omoto et al., 2018, Sonn et al., 2018, Sun et al., 2018, Xu and Xu, 2018, Cichewicz et al., 2017, Hartenstein et al., 2017, Inoshita et al., 2017, Kim et al., 2017, Liu et al., 2017, Niens et al., 2017, Quintero-Espinosa et al., 2017, Wang et al., 2016, Cohn et al., 2015, Gehrke et al., 2015, Hartenstein et al., 2015, Jeong et al., 2015, Kirkhart and Scott, 2015, Krüttner et al., 2015, Linford et al., 2015, Lovick and Hartenstein, 2015, Shankar et al., 2015, Stratoulias and Heino, 2015, Stratoulias and Heino, 2015, Dawydow et al., 2014, Hamilton et al., 2014, Hwang et al., 2014, Schoofs et al., 2014, Shukla et al., 2014, Shukla et al., 2014, Slawson et al., 2014, Yuan et al., 2014, Alekseyenko et al., 2013, Kim and Yim, 2013, Ortega-Arellano et al., 2013, Pech et al., 2013, Riemensperger et al., 2013, Wu et al., 2013, Chen et al., 2012, Duran et al., 2012, Humphrey et al., 2012, Islam et al., 2012, Kim et al., 2012, Liu et al., 2012, Mandilaras and Missirlis, 2012, Marella et al., 2012, Bang et al., 2011, Chiang et al., 2011, Gajula Balija et al., 2011, Kolodziejczyk and Nässel, 2011, Stavropoulos and Young, 2011, Wang et al., 2011, Alekseyenko et al., 2010, Gehrke et al., 2010, Liu and Lu, 2010, White et al., 2010, Wu et al., 2010, Al-Anzi et al., 2009, Venderova et al., 2009, Gao et al., 2008, Imai et al., 2008, Kaplan et al., 2008, Lee et al., 2008, Lee et al., 2008, Nässel et al., 2008, Liu et al., 2007, Ganguly-Fitzgerald et al., 2006, Schroll et al., 2006, Wheeler et al., 2006, Yuan et al., 2006, Melcher and Pankratz, 2005, Riemensperger et al., 2005, Schwaerzel et al., 2003)
Tph-Gal4
Tyrosine Hydroxylase (TH)-GAL4
Tyrosine hydroxylase-GAL4
ple/th-gal4
tyrosine hydoxylase (TH) GAL4
tyrosine hydroxylase (TH)-Gal4
tyrosine-hydroxylase-GAl4
Secondary FlyBase IDs
  • FBtp0020119
  • FBtp0093226
References (328)