Abstract
The Drosophila brain contains about 50 distinct morphological types of dopamine neurons.1,2,3,4 Physiological studies of Drosophila dopamine neurons have been largely limited to one brain region, the mushroom body,5,6,7,8,9,10,11,12,13 where they are implicated in learning.14,15,16,17,18 By comparison, we know little about the physiology of other Drosophila dopamine neurons. Interestingly, a recent whole-brain imaging study found that dopamine neuron activity in several fly brain regions is correlated with locomotion.19 This is notable because many dopamine neurons in the rodent brain are also correlated with locomotion or other movements20,21,22,23,24,25,26,27,28,29,30; however, most rodent studies have focused on learned and rewarded behaviors, and few have investigated dopamine neuron activity during spontaneous (self-timed) movements. In this study, we monitored dopamine neurons in the Drosophila brain during self-timed locomotor movements, focusing on several previously uncharacterized cell types that arborize in the superior-lateral brain, specifically the lateral horn and superior-lateral protocerebrum. We found that activity of all of these dopamine neurons correlated with spontaneous fluctuations in walking speed, with different cell types showing different speed correlations. Some dopamine neurons also responded to odors, but these responses were suppressed by repeated odor encounters. Finally, we found that the same identifiable dopamine neuron can encode different combinations of locomotion and odor in different individuals. If these dopamine neurons promote synaptic plasticity-like the dopamine neurons of the mushroom body-then, their tuning profiles would imply that plasticity depends on a flexible integration of sensory signals, motor signals, and recent experience.
