FB2026_03 , released September 17, 2026
Insertion: Dmel\P{GawB}c309
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General Information
Symbol
Dmel\P{GawB}c309
Species
D. melanogaster
Name
FlyBase ID
FBti0006248
Feature type
transposable_element_insertion_site
Also Known As
c309, c309-GAL4
Description
Inserted Element
P{GawB}
LINE ID
Affected gene(s)
Scer\GAL4
Viability / fertility
fertile, viable
Associated allele(s)
Scer\GAL4c309
Stock availability
1 publicly available
Genomic Location
Chromosomal Location
2-
Sequence Location
Target / Docking site
Member of Large Scale Dataset(s)
Dataset
Description
Detailed Mapping Data
Chromosome (arm)

2-

(Bloomington Drosophila Stock Center, 2003.3.24)
Sequence Location
Orientation
Cytological location (computed by FlyBase)
Cytological location (reported)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct
P{GawB}
Size (Kb)

11.279

Component Allele(s)
Scer\GAL4wB
w+mW.hs
Molecular map
Affected Gene(s)
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
larval ventral nerve cord
(Pfeiffenberger and Allada, 2012)
embryonic/larval mushroom body
(Kester and Nambu, 2011)
adult ventral nerve cord
(Kester and Nambu, 2011)
eye-antennal disc
(Kester and Nambu, 2011)
wandering third instar larval stage
antennal disc | restricted
(Manseau et al., 1997)
medulla anlage
(Manseau et al., 1997)
eye disc | posterior
(Manseau et al., 1997)
adult stage
adult brain | restricted
(Connolly et al., 1996)
adult ventral nerve cord
(Connolly et al., 1996)
adult mushroom body
(Pfeiffenberger and Allada, 2012, Dubnau et al., 2001, Waddell et al., 2000, Connolly et al., 1996)
adult mushroom body | restricted
(Bushey et al., 2009, Zhou et al., 2008, Nitz et al., 2002)
adult mushroom body alpha-lobe
(Krashes et al., 2007)
adult mushroom body beta-lobe
(Krashes et al., 2007)
ellipsoid body
(Pfeiffenberger and Allada, 2012)
gamma Kenyon cell
(Pauls et al., 2010)
alpha/beta Kenyon cell
(Pauls et al., 2010)
alpha'/beta' Kenyon cell
(Pauls et al., 2010)
adult mushroom body gamma-lobe
(Pauls et al., 2010, Aso et al., 2009, Krashes et al., 2007)
adult mushroom body beta'-lobe | restricted
(Krashes et al., 2007)
adult mushroom body alpha'-lobe | restricted
(Krashes et al., 2007)
mushroom body beta lobe core layer | intense
(Aso et al., 2009)
mushroom body alpha lobe core layer | intense
(Aso et al., 2009)
mushroom body beta lobe surface layer | intense
(Aso et al., 2009)
mushroom body alpha lobe surface layer | intense
(Aso et al., 2009)
mushroom body beta lobe posterior layer | intense
(Aso et al., 2009)
mushroom body alpha lobe posterior layer | intense
(Aso et al., 2009)
adult antennal lobe | faint
(Aso et al., 2009)
ellipsoid body | faint
(Aso et al., 2009)
medulla | intense
(Aso et al., 2009)
lobula | faint
(Aso et al., 2009)
lobula plate | faint
(Aso et al., 2009)
adult lateral horn | faint
(Aso et al., 2009)
adult antennal nerve
(Aso et al., 2009)
adult deutocerebrum
(Aso et al., 2009)
adult tritocerebrum
(Aso et al., 2009)
adult gnathal ganglion
(Aso et al., 2009)
glial cell of adult brain
(Aso et al., 2009)
adult pars intercerebralis | faint
(Aso et al., 2009)
adult pars intercerebralis
(Connolly et al., 1996)
adult mushroom body alpha-lobe
(Pauls et al., 2010)
adult mushroom body beta-lobe
(Pauls et al., 2010)
adult mushroom body alpha'-lobe
(Pauls et al., 2010)
adult mushroom body beta'-lobe
(Pauls et al., 2010)
adult stage | male
adult mushroom body alpha-lobe
(Blum et al., 2009)
adult mushroom body beta-lobe
(Blum et al., 2009)
adult mushroom body gamma-lobe
(Blum et al., 2009)
Additional Information
Statement
Reference

Drives expression in the alpha, beta, alpha', beta' and gamma lobes of the mushroom body.

(FlyBase Curators, 2017)

Expression is limited to alpha, beta, and gamma neurons in the adult mushroom body.

(Blum et al., 2009)

Expression is found in only a few alpha'/beta' neurons.

(Krashes et al., 2007)

Expression of ScerGAL4c309 is within a large subset of the Kenyon cells.

(Nitz et al., 2002)

Expression is highest in the mushroom bodies, but is generally widespread.

(Waddell et al., 2000)

In third instar larva, expression in antennal disc restricted to groups of cells.

(Manseau et al., 1997)

Expression in adult brain primarily in corpora pedunculata (mushroom body).

(Connolly et al., 1996)
 
Marker for
Reflects expression of
Reporter construct used in assay
Ecol\lacZUAS.cBa
(Waddell et al., 2000)
shi1.UAS
(Nitz et al., 2002, Dubnau et al., 2001)
Avic\GFPUAS.cUa
(Dubnau et al., 2001)
TbhUAS.cMa
(Zhou et al., 2008)
Fmr1EP3517
(Bushey et al., 2009)
Mmus\Cd8aUAS.cLa.EGFP
(Pauls et al., 2010, Aso et al., 2009, Blum et al., 2009, Krashes et al., 2007)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (10)
Reported As
Symbol Synonym
C309
(Wang et al., 2022, Donlea et al., 2009, Qian et al., 2007, Xia and Tully, 2007, Bhandari et al., 2006)
C309-Gal4
(Yatsenko et al., 2021, Melicharek et al., 2010, Zhou et al., 2008)
P{GAL4}c309
(Koushika et al., 1996, FlyBase, 1992-)
P{GawB}C309
(Connolly et al., 1996, FlyBase, 1992-)
P{GawB}c309
(Kester and Nambu, 2011, Bushey et al., 2009, Bloomington Drosophila Stock Center, 2003.3.24, Koushika et al., 1996, FlyBase, 1992-)
c309
(Matsuno et al., 2026, Rojo-Cortés et al., 2026, Machado Almeida et al., 2021, Deng et al., 2019, Ghezzi et al., 2013, Ueno et al., 2013, Miyashita et al., 2012, Naganos et al., 2012, Pfeiffenberger and Allada, 2012, Bang et al., 2011, Jepson et al., 2011, Kang et al., 2011, Pauls et al., 2010, Pauls et al., 2010, Aso et al., 2009, Blum et al., 2009, van Swinderen et al., 2009, Chen et al., 2008, Hong et al., 2008, Horiuchi et al., 2008, Krashes et al., 2007, Qian et al., 2007, Yamazaki et al., 2007, Joiner et al., 2006, Pitman et al., 2006, Nitz et al., 2002, Rodan et al., 2002, McGuire et al., 2001, Koushika et al., 2000, Waddell et al., 2000, Manseau et al., 1997)
c309-GAL4
(Hidalgo et al., 2021, Bai et al., 2018, Seugnet et al., 2017, Ueno et al., 2017, Tong et al., 2016, Seugnet et al., 2011, Crocker et al., 2010, Lee et al., 2007, Weasner et al., 2007)
c309-Gal4
(O'Hara et al., 2024, Liu et al., 2022, Rojo-Cortés et al., 2022, Hidalgo et al., 2021, Li et al., 2021, Valadas et al., 2018, Afonso et al., 2015, Bai and Sehgal, 2015, Jeong et al., 2015, Shankar et al., 2015, Oh et al., 2014, Ghezzi et al., 2013, Kottler et al., 2013, Kucherenko et al., 2012, Stavropoulos and Young, 2011)
c309GAL4
(Peng et al., 2024)
cb309-GAL4
(Weasner et al., 2007)
Name Synonyms
Secondary FlyBase IDs
  • FBti0004164
  • FBti0004201
References (73)