FlyBase Insertion Report: Dmel\P{GawB}NP1131

General Information

Symbol

Dmel\P{GawB}NP1131

Species

D. melanogaster

Name

FlyBase ID

FBti0034208

Feature type

transposable_element_insertion_site

Also Known As

NP1131, NP1131-GAL4

Description

Inserted Element

P{GawB}

LINE ID

Affected gene(s)

Scer\GAL4

Viability / fertility

Associated allele(s)

Scer\GAL4^NP1131

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

Sequence Location

Target / Docking site

Member of Large Scale Dataset(s)

Dataset

TI_set_P{GawB}.NP

Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w^+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

(Hayashi et al., 2002)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AG218376

(FlyBase, 1992-)

Inserted Element

Construct

P{GawB}

Size (Kb)

11.279

Component Allele(s)

Scer\GAL4^wB

w^+mW.hs

Molecular map

Affected Gene(s)

Insertion may affect gene

Scer\GAL4

(Zhou et al., 2012, Buchanan and Davis, 2010, Pauls et al., 2010, Pauls et al., 2010, Aso et al., 2009, Mao and Davis, 2009, Tanaka et al., 2008)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Scer\GAL4^NP1131

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

surface layer of the larval mushroom body

(Pauls et al., 2010)

pedunculus of larval mushroom body

Comment: surface layer

(Pauls et al., 2010)

larval central nervous system

(Pauls et al., 2010)

embryonic/larval mushroom body

(Olson et al., 2019)

antennal disc

(Olson et al., 2019)

wing hinge primordium

(Olson et al., 2019)

wing pouch | restricted

(Olson et al., 2019)

adult stage

gamma Kenyon cell

(Flybrain Neuron Database et al., 2018.6.28, Yagi et al., 2016, Buchanan and Davis, 2010, Tanaka et al., 2008)

calyx of adult mushroom body

(Tanaka et al., 2008)

adult mushroom body gamma-lobe

(Pauls et al., 2010, Mao and Davis, 2009)

adult mushroom body gamma-lobe | intense

(Aso et al., 2009)

mushroom body beta' anterior-posterior layer

(Aso et al., 2009)

ellipsoid body | intense

(Aso et al., 2009)

nodulus | faint

(Aso et al., 2009)

medulla

(Aso et al., 2009)

adult tritocerebrum | intense

(Aso et al., 2009)

adult gnathal ganglion

(Aso et al., 2009)

adult ellipsoid body ring neuron R4d

(Martín-Peña et al., 2014)

adult pars intercerebralis | intense

(Aso et al., 2009)

alpha'/beta' anterior-posterior Kenyon cell

(Flybrain Neuron Database et al., 2018.6.28)

Kenyon cell

(Flybrain Neuron Database et al., 2018.6.28)

adult mushroom body alpha'-lobe

(Pauls et al., 2010)

adult mushroom body beta'-lobe

(Pauls et al., 2010)

alpha'/beta' Kenyon cell

(Yagi et al., 2016)

alpha/beta core Kenyon cell

(Yagi et al., 2016)

Additional Information

Statement

Reference

Drives expression in neurons of the adult mushroom body gamma-lobe, and in ring neurons R4d.

(FlyBase Curators, 2017)

ScerGAL4^NP1131 drives expression in about 5 ring neurons R4d at 7-8 days of age. Labeled neurons co-express ScerGAL80^ChAT.7.4P.

(Martín-Peña et al., 2014)

Labels a set of 25 embryonic-born gamma neurons on the surface layer of the third instar larvae mushroom body and is already visible at the first instar larval stage.

(Pauls et al., 2010)

Marker for

adult mushroom body gamma-lobe

(Mao and Davis, 2009)

Reflects expression of

ChAT

(Martín-Peña et al., 2014)

Reporter construct used in assay

Mmus\Cd8a^UAS.cLa.EGFP

(Pauls et al., 2010, Aso et al., 2009, Mao and Davis, 2009, Tanaka et al., 2008)

Avic\GFP^S65T.UAS

(Tanaka et al., 2008)

Nf1^UAS.cBa

(Buchanan and Davis, 2010)

Avic\GFP^{UAS.Tag:NLS(SV40-largeT)}

(Martín-Peña et al., 2014)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Kyoto

103898

y^* w^*; P{GawB}NP1131 / CyO, P{UAS-lacZ.UW14}UW14

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

1131

(Olson et al., 2019)

NP 1131

(Pauls et al., 2010)

NP1131-GAL4

(Schwartz et al., 2023, Sabandal et al., 2022, Sabandal et al., 2020, Aranda et al., 2017, Ries et al., 2017, Crocker et al., 2016, Drago and Davis, 2016, Perrat et al., 2013, Mao and Davis, 2009)

NP1131-Gal4

(Gouzi et al., 2018, Kirkhart and Scott, 2015, Kanellopoulos et al., 2012, Zhou et al., 2012)

NP1131-gal4

(Buchanan and Davis, 2010)

P{GawB}NP1131

(Martín-Peña et al., 2014, FlyBase, 1992-)

P{GawB}NP1131-3-1

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

References (42)

Report Sections

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