FlyBase Insertion Report: Dmel\P{GawB}NP6520

General Information

Symbol

Dmel\P{GawB}NP6520

Species

D. melanogaster

Name

FlyBase ID

FBti0143411

Feature type

transposable_element_insertion_site

Also Known As

NP6520-Gal4, NP6520

Description

Inserted Element

P{GawB}

LINE ID

Affected gene(s)

Scer\GAL4

Viability / fertility

Associated allele(s)

Scer\GAL4^NP6520

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

Sequence Location

Target / Docking site

Member of Large Scale Dataset(s)

Dataset

TI_set_P{GawB}.NP

Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w^+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

(Hayashi et al., 2002)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

P{GawB}

(Ng et al., 2011)

Size (Kb)

11.279

Component Allele(s)

Scer\GAL4^wB

w^+mW.hs

Molecular map

Affected Gene(s)

Insertion may affect gene

Scer\GAL4

(Ng et al., 2011, Awasaki et al., 2008)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Scer\GAL4^NP6520

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

larval neuropil glial cell | subset

(Losada-Perez et al., 2016)

third instar larval stage

embryonic/larval ensheathing glial cell

(Spéder and Brand, 2014)

late third instar larval stage -- pupal stage P6

transient interhemispheric fibrous ring

(Lovick et al., 2017)

adult stage

adult ensheathing glial cell

(Melom and Littleton, 2013, Awasaki et al., 2008)

adult brain cell body glial cell | faint

(Awasaki et al., 2008)

Additional Information

Statement

Reference

Drives expression in ensheathing glial cells.

(FlyBase Curators, 2017)

Scer\GAL4^NP6520 drives expression in a subset of ensheathing glia in the larval and pupal central brain.

(Lovick et al., 2017)

The larval neuropil glial cells are Gs2-positive and comprise ScerGAL4^alrm.PD-positive, pros-positive and ScerGAL4^NP6520-positive, pros-negative subsets.

(Losada-Perez et al., 2016)

ScerGAL4^NP6520 drives expression in ensheathing glia, and faintly in cortex glia.

(Awasaki et al., 2008)

Marker for

adult ensheathing glial cell

(Melom and Littleton, 2013)

ensheathing glial cell

(Lovick et al., 2017)

Reflects expression of

Reporter construct used in assay

Mmus\Cd8a^UAS.cLa.EGFP

(Awasaki et al., 2008)

Mmus\Cd8a^UAS.mRFP1

(Awasaki et al., 2008)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Kyoto

105240

y^* w^*; P{GawB}NP6520 / TM6, P{UAS-lacZ.UW23-1}UW23-1

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

EG GAL4

(Miyashita et al., 2023)

EG-Gal4

(Coll-Tané et al., 2021)

NP6520

(Miyashita et al., 2023, Kato et al., 2020, Nakano et al., 2019, Delgado et al., 2018, Losada-Perez et al., 2016, Schulz et al., 2015, Stratoulias and Heino, 2015, Stratoulias and Heino, 2015, Ng et al., 2011, Awasaki et al., 2008)

NP6520-GAL4

(Ma et al., 2024, Kowada et al., 2021, Kim et al., 2020, Long and Giebultowicz, 2017, Spéder and Brand, 2014)

NP6520-Gal4

(Clayworth et al., 2024, Petitgas et al., 2024, Shu et al., 2023, Coll-Tané et al., 2021, Walkowicz et al., 2021, Deng et al., 2019, Galagovsky et al., 2018, Kunduri et al., 2018, Aw et al., 2017, Foo et al., 2017, Lovick et al., 2017, Richier et al., 2017, Ou et al., 2016, Okamoto and Nishimura, 2015, Omoto et al., 2015)

P{GawB}NP6520

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

References (33)

Report Sections

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