FlyBase Allele Report: Dmel\Diap1[UAS.Tag:MYC.cBa]

General Information

Symbol

Dmel\Diap1^{UAS.Tag:MYC.cBa}

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Betz

FlyBase ID

FBal0246254

Feature type

allele

Associated gene

Dmel\Diap1

Associated Insertion(s)

Carried in Construct

P{UAS-Diap1.MYC.B}

Also Known As

UAS-dIAP1, UAS-myc-dIAP1

Key Links

Transgenic product class

wild_type

(Ryoo, 2013.7.26)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Ryoo, 2013.7.26)

Mutagen

in vitro construct

(Ryoo, 2013.7.26, Betz et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-Diap1.MYC.B}

Cytology

Description

UAS regulatory sequences drive expression of Diap1 tagged with 6 tandem repeats of Tag:MYC.

(Ryoo, 2013.7.26)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Diap1

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^C57

(Liu et al., 2010)

Phenotype Manifest In

synapse, with Scer\GAL4^C57

(Liu et al., 2010)

Detailed Description

Statement

Reference

The eyes of flies expressing th^{Scer\UAS.T:Hsap\MYC.cBa} under the control of Scer\GAL4^GMR.PF are similar in size to controls.

(Pechkovsky et al., 2013)

Overexpression of BacA\p35^Scer\UAS.cHa in presynaptic neurons under the control of Scer\GAL4^OK6 has no obvious effect on synapse elimination.

Overexpression of BacA\p35^Scer\UAS.cHa in postsynaptic neurons under the control of Scer\GAL4^C57 results in strong arrest of synapse elimination at 9 hours after puparium formation.

(Liu et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Diap1^{UAS.Tag:MYC.cBa}, Scer\GAL4^en-e16E is a suppressor | partially of abnormal size | third instar larval stage phenotype of Scer\GAL4^en-e16E, dome^ΔCYT.UAS

(Recasens-Alvarez et al., 2017)

Diap1^{UAS.Tag:MYC.cBa}, Scer\GAL4^en-e16E is a suppressor | partially of increased cell death | third instar larval stage phenotype of Scer\GAL4^en-e16E, dome^ΔCYT.UAS

(Recasens-Alvarez et al., 2017)

Diap1^{UAS.Tag:MYC.cBa}, Scer\GAL4^GMR.PF is a suppressor | partially of visible | adult stage phenotype of HAdV-C\E4orf4^UAS.cPa, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

Diap1^{UAS.Tag:MYC.cBa}, Scer\GAL4^GMR.PF is a suppressor | partially of abnormal size | adult stage phenotype of HAdV-C\E4orf4^UAS.cPa, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

Diap1^{UAS.Tag:MYC.cBa}, Scer\GAL4^GMR.PF is a suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.PF, rpr^UAS.cZa

(Pechkovsky et al., 2013)

Diap1^{UAS.Tag:MYC.cBa}, Scer\GAL4^GMR.PF is a suppressor of partially lethal - majority die phenotype of Scer\GAL4^GMR.PF, rpr^UAS.cZa

(Pechkovsky et al., 2013)

Scer\GAL4^Tub.PU/Diap1^{UAS.Tag:MYC.cBa} is a suppressor of increased cell death | conditional | third instar larval stage phenotype of Stat92E³⁹⁷

(Betz et al., 2008)

NOT Suppressor of

Statement

Reference

Diap1^{UAS.Tag:MYC.cBa}, Scer\GAL4^{VP16.nanos.UTR} is a non-suppressor of decreased cell number | embryonic stage phenotype of AIF^UAS.Tag:MYC, DNaseII^UAS.cTa, Scer\GAL4^{VP16.nanos.UTR}

(Tarayrah-Ibraheim et al., 2021)

Phenotype Manifest In

Suppressor of

Statement

Reference

Diap1^{UAS.Tag:MYC.cBa}, Scer\GAL4^en-e16E is a suppressor | partially of wing disc posterior compartment | third instar larval stage phenotype of Scer\GAL4^en-e16E, dome^ΔCYT.UAS

(Recasens-Alvarez et al., 2017)

Diap1^{UAS.Tag:MYC.cBa}, Scer\GAL4^GMR.PF is a suppressor | partially of eye phenotype of HAdV-C\E4orf4^UAS.cPa, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

Diap1^{UAS.Tag:MYC.cBa}, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, rpr^UAS.cZa

(Pechkovsky et al., 2013)

Diap1^{UAS.Tag:MYC.cBa}, Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of Scer\GAL4^GMR.PF, rpr^UAS.cZa

(Pechkovsky et al., 2013)

Scer\GAL4^Tub.PU/Diap1^{UAS.Tag:MYC.cBa} is a suppressor of wing disc | conditional | third instar larval stage phenotype of Stat92E³⁹⁷

(Betz et al., 2008)

NOT Suppressor of

Statement

Reference

Diap1^{UAS.Tag:MYC.cBa}, Scer\GAL4^{VP16.nanos.UTR} is a non-suppressor of primordial germ cell | embryonic stage | decreased number phenotype of AIF^UAS.Tag:MYC, DNaseII^UAS.cTa, Scer\GAL4^{VP16.nanos.UTR}

(Tarayrah-Ibraheim et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of Diap1^{Scer\UAS.T:Hsap\MYC.cBa} partially suppresses the severe decrease in size and the severe increase in apoptosis induced in the third instar wing disc posterior compartment by the expression of dome^{ΔCYT.Scer\UAS} under the control of Scer\GAL4^en-e16E.

(Recasens-Alvarez et al., 2017)

Expression of th^{Scer\UAS.T:Hsap\MYC.cBa} almost completely restores the eye phenotypes seen when rpr^Scer\UAS.cZa is expressed under the control of Scer\GAL4^GMR.PF at 29[o]C. Eye size is 98% of control and no roughness or other morphological abnormalities are observed. The loss of viability seen in flies expressing rpr^Scer\UAS.cZa is rescued upon co-expression of th^{Scer\UAS.T:Hsap\MYC.cBa}.

(Pechkovsky et al., 2013)

When th^Scer\UAS.cBa is over-expressed in the wing disc under the control of Scer\GAL4^tub.PU from first instar larval stage (by heat-shock induction to inactivate Scer\GAL80^αTub84B.PL), Stat92E³⁹⁷ mutant clones take up 7.7% of the wing pouch after x-irradiation treatment (5,000 rads daily for 3 days). Low levels of cell death are observed.

(Betz et al., 2008)

Xenogenetic Interactions

Statement

Reference

Expression of th^{Scer\UAS.T:Hsap\MYC.cBa} partially rescues the eye phenotypes seen when Zzzz\E4orf4^Scer\UAS.cPa is expressed under the control of Scer\GAL4^GMR.PF at 29[o]C. Eye size is restored to 75% and 87% of controls in males and females respectively. Eyes still appear rough and shape is disrupted.

(Pechkovsky et al., 2013)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Diap1^{Scer\UAS.T:Hsap\MYC.cBa}

Diap1^{UAS.Tag:MYC.cBa}

th^{Scer\UAS.T:Hsap\MYC.cBa}

th^Scer\UAS.cBa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Betz

Secondary FlyBase IDs

References (8)

Report Sections

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