Amino acid replacement: N117K.
UASt regulatory sequences drive expression of an in vivo reporter of Diap1 protein turnover. The reporter consists of Diap1 coding sequence that is tagged with the Venus fluorescent protein at its C-terminus. In addition, to ensure that the protein does not interfere with caspase-dependent cell death signaling when it is expressed, it contains containing two point mutations (amino acid replacements N117K and H283Y) which have been shown to block binding to activated Drice and Dcp-1 and impair binding to pro-Dronc respectively.
Amino acid replacement: H283Y.