Open Close
General Information
Symbol
P{UAS-DIAP1.PRAP}
FlyBase ID
FBtp0053781
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
Construct components
Component allele Diap1PRAP.UAS.Venus
Product class / Tool use(s)
Description

UASt regulatory sequences drive expression of an in vivo reporter of Diap1 protein turnover. The reporter consists of Diap1 coding sequence that is tagged with the Venus fluorescent protein at its C-terminus. In addition, to ensure that the protein does not interfere with caspase-dependent cell death signaling when it is expressed, it contains containing two point mutations (amino acid replacements N117K and H283Y) which have been shown to block binding to activated Drice and Dcp-1 and impair binding to pro-Dronc respectively.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
binary
component
molecular
tag
Component Alleles
Reference(s)
Molecular data

UASt regulatory sequences drive expression of an in vivo reporter of Diap1 protein turnover. The reporter consists of Diap1 coding sequence that is tagged with the Venus fluorescent protein at its C-terminus. In addition, to ensure that the protein does not interfere with caspase-dependent cell death signaling when it is expressed, it contains containing two point mutations (amino acid replacements N117K and H283Y) which have been shown to block binding to activated Drice and Dcp-1 and impair binding to pro-Dronc respectively.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
P{UAS-DIAP1.PRAP}
Secondary FlyBase IDs
    References (2)