FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{UAS-DIAP1.PRAP}
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General Information
Symbol
P{UAS-DIAP1.PRAP}
FlyBase ID
FBtp0053781
Feature type
transgenic_transposable_element
Component Allele(s)
Diap1PRAP.UAS.Venus
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Diap1PRAP.UAS.Venus
Transgenic product class
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Diap1
Tagged with
Venus
Description

UASt regulatory sequences drive expression of an in vivo reporter of Diap1 protein turnover. The reporter consists of Diap1 coding sequence that is tagged with the Venus fluorescent protein at its C-terminus. In addition, to ensure that the protein does not interfere with caspase-dependent cell death signaling when it is expressed, it contains containing two point mutations (amino acid replacements N117K and H283Y) which have been shown to block binding to activated Drice and Dcp-1 and impair binding to pro-Dronc respectively.

(Koto et al., 2009)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
PRAP
(Koto et al., 2009)
P{UAS-DIAP1.PRAP}
(FlyBase, 1992-)
UAS-PRAP
(Koto et al., 2009)
Secondary FlyBase IDs
    References (3)