FlyBase Allele Report: Scer\GAL4[twi.2PE]

General Information

Symbol

Scer\GAL4^twi.2PE

Species

S. cerevisiae

Name

FlyBase ID

FBal0060664

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{GAL4-twi.2xPE}

Also Known As

2xPE-twi-GAL4, twist-Gal4, 2xPE-GAL4, twi.2xPE-GAL4, twist2xPE-Gal4, twi::Gal4, twi-GAL4

Allele class

Mutagen

in vitro construct

Nature of the Allele

Allele class

Mutagen

in vitro construct

(Schubiger, 1997.3.14)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Ecol\lacZ^twi.2PE

(Schubiger, 1997.3.14)

Carried in construct

P{GAL4-twi.2xPE}

Cytology

Nature of the lesion

Statement

Reference

Construct: BamHI and XbaI were used to remove Ecol\lacZ and replace it with Scer\GAL4^wB.

(Schubiger, 1997.3.14)

Allele components

Product class / Tool use(s)

Regulatory region(s)

twi

Encoded product / tool

GAL4

binary expression system - driver

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

mesoderm

(Mendoza-García et al., 2017)

embryonic stage 5

organism | ventral

(Schubiger, 1997.3.14)

Additional Information

Statement

Reference

Drives mesodermal expression

(FlyBase Curators, 2017)

In embryos at cellular blastoderm, expressed at high levels in the ventralmost 12-14 cells, with sharp boundaries at lateral borders.

(Schubiger, 1997.3.14)

Marker for

Reflects expression of

twi

(Mendoza-García et al., 2017, Schubiger, 1997.3.14)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration | embryonic stage 8, with Rac1^V12.UAS

(van Impel et al., 2009)

abnormal cell migration | embryonic stage 8, with Scer\GAL4^Mef2.PR, pbl^{DH-PH.UAS.Tag:HA}

(van Impel et al., 2009)

abnormal cell migration | embryonic stage 8, with Scer\GAL4^Mef2.PR, pbl^{ΔN-term.UAS.Tag:HA}

(van Impel et al., 2009)

abnormal cytokinesis | embryonic stage 8, with Scer\GAL4^Mef2.PR, pbl^{ΔN-term.UAS.Tag:HA}

(van Impel et al., 2009)

decreased cell number | embryonic stage, with Egfr^DN.UAS.cBa, Scer\GAL4^how-24B

(Schwarz et al., 2018)

decreased cell number | embryonic stage, with rho^UAS.cdCa

(Schwarz et al., 2018)

female sterile, with shd¹/shd², shd^UAS.cPa

(Petryk et al., 2003)

increased cell number | embryonic stage, with edl^UAS.cYa

(Schwarz et al., 2018)

lethal, with E2f1^UAS.cNa

(Morris et al., 2006)

lethal, with rpr^UAS.cZa

(Morris et al., 2006)

lethal - all die before end of first instar larval stage, with Mes2^UAS.cZa

(Zimmermann et al., 2006)

some die during first instar larval stage, with Mes2^UAS.cZa

(Zimmermann et al., 2006)

viable, with shd¹/shd², shd^UAS.cPa

(Petryk et al., 2003)

Phenotype Manifest In

cardioblast, with HLH54F^UAS.cIa, Scer\GAL4^how-24B

(Ismat et al., 2010)

cardioblast | increased number, with Doc2^UAS.cRa

(Reim and Frasch, 2005)

corpus cardiacum primordium, with E(spl)m8-HLH^UAS.cUa

(Park et al., 2011)

corpus cardiacum primordium, with N^int.U.UAS

(Park et al., 2011)

corpus cardiacum primordium, with twi^UAS.cBa

(Park et al., 2011)

egg chamber, with shd¹/shd², shd^UAS.cPa

(Petryk et al., 2003)

embryonic/larval alary muscle | embryonic stage, with org-1^UAS.cSa

(Boukhatmi et al., 2014)

embryonic/larval midgut muscle, with HLH54F^UAS.cIa, Scer\GAL4^how-24B

(Ismat et al., 2010)

embryonic/larval somatic muscle, with Scer\GAL4^how-24B, org-1^UAS.cSa

(Schaub et al., 2012)

embryonic/larval thoracic alary-related muscle | embryonic stage, with org-1^UAS.cSa

(Boukhatmi et al., 2014)

embryonic cardioblast, with Egfr^DN.UAS.cBa, Scer\GAL4^how-24B

(Schwarz et al., 2018)

embryonic cardioblast, with pyr^UAS.Tag:HA

(Klingseisen et al., 2009)

embryonic cardioblast | increased number, with edl^UAS.cYa

(Schwarz et al., 2018)

embryonic ostial cardioblast, with rho^UAS.cdCa

(Schwarz et al., 2018)

embryonic ostial cardioblast | increased number, with edl^UAS.cYa

(Schwarz et al., 2018)

embryonic pericardial cell, with Egfr^DN.UAS.cBa

(Schwarz et al., 2018)

embryonic somatic muscle, with HLH54F^UAS.cIa, Scer\GAL4^how-24B

(Ismat et al., 2010)

heart primordium, with edl^UAS.cYa

(Schwarz et al., 2018)

heart primordium, with Egfr^DN.UAS.cBa

(Schwarz et al., 2018)

heart primordium, with Egfr^DN.UAS.cBa, Scer\GAL4^how-24B

(Schwarz et al., 2018)

heart primordium, with rho^UAS.cdCa

(Schwarz et al., 2018)

mesoderm, with pyr^UAS.Tag:HA

(Klingseisen et al., 2009)

mesoderm | embryonic stage 8, with Rac1^V12.UAS

(van Impel et al., 2009)

mesoderm | embryonic stage 8, with Scer\GAL4^Mef2.PR, pbl^{DH-PH.UAS.Tag:HA}

(van Impel et al., 2009)

mesoderm | embryonic stage 8, with Scer\GAL4^Mef2.PR, pbl^{ΔN-term.UAS.Tag:HA}

(van Impel et al., 2009)

muscle founder cell & visceral muscle primordium, with Alk^UAS.DN

(Lee et al., 2003)

muscle founder cell & visceral muscle primordium | ectopic, with jeb^UAS.cWa

(Lee et al., 2003)

muscle founder cell & visceral muscle primordium | ectopic, with Ras85D^V12.UAS

(Lee et al., 2003)

visceral mesoderm & muscle founder cell | ectopic, with Hsap\RAF1^gof.UAS

(Lee et al., 2003)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^twi.2PE, pbl^{ΔBRCT.UAS.Tag:MYC}, pbl³ has abnormal cell migration | embryonic stage phenotype, suppressible by Rac1^UAS.cLa

(van Impel et al., 2009)

NOT suppressed by

Statement

Reference

Scer\GAL4^twi.2PE, pbl^{ΔBRCT.UAS.Tag:MYC}, pbl³ has abnormal cell migration | embryonic stage phenotype, non-suppressible by Rho1^UAS.cMa

(van Impel et al., 2009)

Enhancer of

Statement

Reference

Scer\GAL4^twi.2PE/Rac1^N17.UAS is an enhancer of abnormal cell migration | embryonic stage phenotype of pbl⁵

(van Impel et al., 2009)

Scer\GAL4^twi.2PE/Rac1^V12.UAS is an enhancer of abnormal cell migration | embryonic stage phenotype of pbl⁵

(van Impel et al., 2009)

NOT Enhancer of

Statement

Reference

Scer\GAL4^twi.2PE/Rho1^V14.UAS is a non-enhancer of abnormal cell migration | embryonic stage phenotype of pbl⁵

(van Impel et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

Doc2^UAS.cRa, Scer\GAL4^twi.2PE has cardioblast | increased number phenotype, enhanceable by pnr^UAS.cHa/tin^UAS.cYa, Scer\GAL4^twi.2PE

(Reim and Frasch, 2005)

Suppressed by

Statement

Reference

Scer\GAL4^twi.2PE, pbl^{ΔBRCT.UAS.Tag:MYC}, pbl³ has mesoderm phenotype, suppressible by Rac1^UAS.cLa

(van Impel et al., 2009)

NOT suppressed by

Statement

Reference

Scer\GAL4^twi.2PE, pbl^{ΔBRCT.UAS.Tag:MYC}, pbl³ has mesoderm phenotype, non-suppressible by Rho1^UAS.cMa

(van Impel et al., 2009)

Enhancer of

Statement

Reference

Scer\GAL4^twi.2PE/Rac1^N17.UAS is an enhancer of mesoderm phenotype of pbl⁵

(van Impel et al., 2009)

Scer\GAL4^twi.2PE/Rac1^V12.UAS is an enhancer of mesoderm phenotype of pbl⁵

(van Impel et al., 2009)

pnr^UAS.cHa/tin^UAS.cYa, Scer\GAL4^twi.2PE is an enhancer of cardioblast | increased number phenotype of Doc2^UAS.cRa, Scer\GAL4^twi.2PE

(Reim and Frasch, 2005)

NOT Enhancer of

Statement

Reference

Scer\GAL4^twi.2PE/Rho1^V14.UAS is a non-enhancer of mesoderm phenotype of pbl⁵

(van Impel et al., 2009)

Suppressor of

Statement

Reference

Ras85D^V12.UAS/Scer\GAL4^twi.2PE is a suppressor | partially of visceral mesoderm phenotype of jeb^-c1

(Lee et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

pbl^UAS.Tag:HA/Scer\GAL4^twi.2PE rescues pbl³

(van Impel et al., 2009)

Scer\GAL4^twi.2PE/pbl^{DH-PH.UAS.Tag:HA} rescues pbl³

(van Impel et al., 2009)

Partially rescues

pbl^{ΔBRCT.UAS.Tag:MYC}/Scer\GAL4^twi.2PE partially rescues pbl³

(van Impel et al., 2009)

pbl^{ΔC-term.UAS.Tag:HA}/Scer\GAL4^twi.2PE partially rescues pbl³

(van Impel et al., 2009)

Scer\GAL4^twi.2PE/shd^UAS.cPa partially rescues shd¹/shd²

(Petryk et al., 2003)

Fails to rescue

pbl^{ΔBRCT.UAS.Tag:MYC}/Scer\GAL4^twi.2PE fails to rescue pbl³

(van Impel et al., 2009)

Scer\GAL4^twi.2PE/pbl^{ΔN-term.UAS.Tag:HA} fails to rescue pbl³

(van Impel et al., 2009)

Scer\GAL4^twi.2PE/pbl^{DH.UAS.Tag:HA} fails to rescue pbl³

(van Impel et al., 2009)

pbl^{ΔC-term.UAS.Tag:HA}/Scer\GAL4^twi.2PE fails to rescue pbl³

(van Impel et al., 2009)

Scer\GAL4^twi.2PE/pbl^{DH-PH-V531D.UAS.Tag:HA} fails to rescue pbl³

(van Impel et al., 2009)

Scer\GAL4^twi.2PE/wit^UAS.cMa fails to rescue wit^unspecified

(Marques et al., 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (9)

Bloomington

2517

y¹ w^*; P{GAL4-twi.2xPE}2

58804

y¹ w^*; P{GAL4-twi.2xPE}3

84311

w^*; P{GAL4-twi.2xPE}1; P{UAS-GC3Ai}3

84290

w^*; P{GAL4-twi.2xPE}1/CyO; P{UAS-CC3Ai}3

84332

w^*; P{GAL4-twi.2xPE}1/CyO; P{UAS-VC3Ai}3

25707

P{UAS-Dcr-2.D}1, w¹¹¹⁸; P{GAL4-twi.2xPE}1

67053

w^*; P{GAL4-twi.2xPE}1/CyO; P{UAS-3xFLAG.dCas9.VPR}attP2

67084

w^*; P{GAL4-twi.2xPE}1/CyO; P{UAS-Cas9.P2}attP2/TM6B, Tb¹

Kyoto

107376

y¹ w^*; P{GAL4-twi.2xPE}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

GAL4^twi.2PE

Scer\GAL4^twi.2PE

Name Synonyms

Secondary FlyBase IDs

References (33)

Report Sections

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