FlyBase Allele Report: Scer\GAL4[twi.2PE]

General Information

Symbol

Scer\GAL4^twi.2PE

Species

S. cerevisiae

Name

FlyBase ID

FBal0060664

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{GAL4-twi.2xPE}

Also Known As

2xPE-GAL4, 2xPE-twi-GAL4, twist-Gal4, twi.2xPE-GAL4, twi::Gal4, twi-GAL4, twist2xPE-Gal4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Schubiger, 1997.3.14)

Progenitor genotype

Ecol\lacZ^twi.2PE

(Schubiger, 1997.3.14)

Carried in construct

P{GAL4-twi.2xPE}

Cytology

Description

Construct: BamHI and XbaI were used to remove Ecol\lacZ and replace it with Scer\GAL4^wB.

(Schubiger, 1997.3.14)

Allele components

Component

Use(s)

Regulatory region(s)

twi

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

mesoderm

(Mendoza-García et al., 2017)

embryonic stage 5

organism | ventral

(Schubiger, 1997.3.14)

Additional Information

Statement

Reference

Drives mesodermal expression

(FlyBase Curators, 2017)

In embryos at cellular blastoderm, expressed at high levels in the ventralmost 12-14 cells, with sharp boundaries at lateral borders.

(Schubiger, 1997.3.14)

Marker for

Reflects expression of

twi

(Mendoza-García et al., 2017, Schubiger, 1997.3.14)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration | embryonic stage 8, with Rac1^V12.UAS

(van Impel et al., 2009)

abnormal cell migration | embryonic stage 8, with Scer\GAL4^Mef2.PR, pbl^{DH-PH.UAS.Tag:HA}

(van Impel et al., 2009)

abnormal cell migration | embryonic stage 8, with Scer\GAL4^Mef2.PR, pbl^{ΔN-term.UAS.Tag:HA}

(van Impel et al., 2009)

abnormal cell number | embryonic stage, with CAP^HM05250, Scer\GAL4^how-24B

(Jammrath et al., 2020)

abnormal cytokinesis | embryonic stage 8, with Scer\GAL4^Mef2.PR, pbl^{ΔN-term.UAS.Tag:HA}

(van Impel et al., 2009)

decreased cell number | embryonic stage 16, with Scer\GAL4^Mef2.247, Spyo\Cas9^UAS.P2, Ubx^{UAS.gRNAx4.pCFD6}

(Domsch et al., 2021)

decreased cell number | embryonic stage, with Egfr^DN.UAS.cBa, Scer\GAL4^how-24B

(Schwarz et al., 2018)

decreased cell number | embryonic stage, with rho^UAS.cdCa

(Schwarz et al., 2018)

female sterile, with shd¹/shd², shd^UAS.cPa

(Petryk et al., 2003)

increased cell number | embryonic stage, with edl^UAS.cYa

(Schwarz et al., 2018)

lethal, with E2f1^UAS.cNa

(Morris et al., 2006)

lethal, with rpr^UAS.cZa

(Morris et al., 2006)

lethal - all die before end of first instar larval stage, with Mes2^UAS.cZa

(Zimmermann et al., 2006)

some die during first instar larval stage, with Mes2^UAS.cZa

(Zimmermann et al., 2006)

viable, with shd¹/shd², shd^UAS.cPa

(Petryk et al., 2003)

Phenotype Manifest In

corpus cardiacum primordium, with E(spl)m8-HLH^UAS.cUa

(Park et al., 2011)

corpus cardiacum primordium, with N^CA.UAS.cUa

(Park et al., 2011)

corpus cardiacum primordium, with twi^UAS.cBa

(Park et al., 2011)

dorsal vessel wall cell, with HLH54F^UAS.cIa, Scer\GAL4^how-24B

(Ismat et al., 2010)

dorsal vessel wall cell | increased number, with Doc2^UAS.cRa

(Reim and Frasch, 2005)

egg chamber, with shd¹/shd², shd^UAS.cPa

(Petryk et al., 2003)

embryonic/larval alary muscle cell | embryonic stage, with org-1^UAS.cSa

(Boukhatmi et al., 2014)

embryonic/larval hypodermal muscle cell | decreased number | embryonic stage 16, with Scer\GAL4^Mef2.247, Spyo\Cas9^UAS.P2, Ubx^{UAS.gRNAx4.pCFD6}

(Domsch et al., 2021)

embryonic/larval hypodermal muscle cell | embryonic stage 16, with Scer\GAL4^Mef2.247, Spyo\Cas9^UAS.P2, Ubx^{UAS.gRNAx4.pCFD6}

(Domsch et al., 2021)

embryonic/larval midgut muscle cell, with HLH54F^UAS.cIa, Scer\GAL4^how-24B

(Ismat et al., 2010)

embryonic/larval thoracic alary-related muscle cell | embryonic stage, with org-1^UAS.cSa

(Boukhatmi et al., 2014)

embryonic cardioblast, with CAP^HM05250, Scer\GAL4^how-24B

(Jammrath et al., 2020)

embryonic cardioblast, with Egfr^DN.UAS.cBa, Scer\GAL4^how-24B

(Schwarz et al., 2018)

embryonic cardioblast, with pyr^UAS.Tag:HA

(Klingseisen et al., 2009)

embryonic cardioblast | increased number, with edl^UAS.cYa

(Schwarz et al., 2018)

embryonic heart ostial cardioblast, with rho^UAS.cdCa

(Schwarz et al., 2018)

embryonic heart ostial cardioblast | increased number, with edl^UAS.cYa

(Schwarz et al., 2018)

embryonic pericardial cell, with Egfr^DN.UAS.cBa

(Schwarz et al., 2018)

embryonic somatic muscle cell, with HLH54F^UAS.cIa, Scer\GAL4^how-24B

(Ismat et al., 2010)

heart primordium, with edl^UAS.cYa

(Schwarz et al., 2018)

heart primordium, with Egfr^DN.UAS.cBa

(Schwarz et al., 2018)

heart primordium, with Egfr^DN.UAS.cBa, Scer\GAL4^how-24B

(Schwarz et al., 2018)

heart primordium, with rho^UAS.cdCa

(Schwarz et al., 2018)

larval somatic muscle cell, with Scer\GAL4^how-24B, org-1^UAS.cSa

(Schaub et al., 2012)

mesoderm, with pyr^UAS.Tag:HA

(Klingseisen et al., 2009)

mesoderm | embryonic stage 8, with Rac1^V12.UAS

(van Impel et al., 2009)

mesoderm | embryonic stage 8, with Scer\GAL4^Mef2.PR, pbl^{DH-PH.UAS.Tag:HA}

(van Impel et al., 2009)

mesoderm | embryonic stage 8, with Scer\GAL4^Mef2.PR, pbl^{ΔN-term.UAS.Tag:HA}

(van Impel et al., 2009)

muscle founder cell & visceral muscle primordium, with Alk^UAS.DN

(Lee et al., 2003)

muscle founder cell & visceral muscle primordium | ectopic, with jeb^UAS.cWa

(Lee et al., 2003)

muscle founder cell & visceral muscle primordium | ectopic, with Ras85D^V12.UAS

(Lee et al., 2003)

visceral mesoderm & muscle founder cell | ectopic, with Hsap\RAF1^gof.UAS

(Lee et al., 2003)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^twi.2PE, pbl^{ΔBRCT.UAS.Tag:MYC}, pbl³ has abnormal cell migration | embryonic stage phenotype, suppressible by Rac1^UAS.cLa

(van Impel et al., 2009)

NOT suppressed by

Statement

Reference

Scer\GAL4^twi.2PE, pbl^{ΔBRCT.UAS.Tag:MYC}, pbl³ has abnormal cell migration | embryonic stage phenotype, non-suppressible by Rho1^UAS.cMa

(van Impel et al., 2009)

Enhancer of

Statement

Reference

Scer\GAL4^twi.2PE/Rac1^N17.UAS is an enhancer of abnormal cell migration | embryonic stage phenotype of pbl⁵

(van Impel et al., 2009)

Scer\GAL4^twi.2PE/Rac1^V12.UAS is an enhancer of abnormal cell migration | embryonic stage phenotype of pbl⁵

(van Impel et al., 2009)

NOT Enhancer of

Statement

Reference

Scer\GAL4^twi.2PE/Rho1^V14.UAS is a non-enhancer of abnormal cell migration | embryonic stage phenotype of pbl⁵

(van Impel et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

Doc2^UAS.cRa, Scer\GAL4^twi.2PE has dorsal vessel wall cell | increased number phenotype, enhanceable by pnr^UAS.cHa/tin^UAS.cYa, Scer\GAL4^twi.2PE

(Reim and Frasch, 2005)

Suppressed by

Statement

Reference

Scer\GAL4^twi.2PE, pbl^{ΔBRCT.UAS.Tag:MYC}, pbl³ has mesoderm phenotype, suppressible by Rac1^UAS.cLa

(van Impel et al., 2009)

NOT suppressed by

Statement

Reference

Scer\GAL4^twi.2PE, pbl^{ΔBRCT.UAS.Tag:MYC}, pbl³ has mesoderm phenotype, non-suppressible by Rho1^UAS.cMa

(van Impel et al., 2009)

Enhancer of

Statement

Reference

Scer\GAL4^twi.2PE/Rac1^N17.UAS is an enhancer of mesoderm phenotype of pbl⁵

(van Impel et al., 2009)

Scer\GAL4^twi.2PE/Rac1^V12.UAS is an enhancer of mesoderm phenotype of pbl⁵

(van Impel et al., 2009)

pnr^UAS.cHa/tin^UAS.cYa, Scer\GAL4^twi.2PE is an enhancer of dorsal vessel wall cell | increased number phenotype of Doc2^UAS.cRa, Scer\GAL4^twi.2PE

(Reim and Frasch, 2005)

NOT Enhancer of

Statement

Reference

Scer\GAL4^twi.2PE/Rho1^V14.UAS is a non-enhancer of mesoderm phenotype of pbl⁵

(van Impel et al., 2009)

Suppressor of

Statement

Reference

Ras85D^V12.UAS/Scer\GAL4^twi.2PE is a suppressor of visceral trunk mesoderm phenotype of jeb^-c1

(Lee et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

pbl^UAS.Tag:HA/Scer\GAL4^twi.2PE rescues pbl³

(van Impel et al., 2009)

Scer\GAL4^twi.2PE/pbl^{DH-PH.UAS.Tag:HA} rescues pbl³

(van Impel et al., 2009)

Partially rescues

pbl^{ΔBRCT.UAS.Tag:MYC}/Scer\GAL4^twi.2PE partially rescues pbl³

(van Impel et al., 2009)

pbl^{ΔC-term.UAS.Tag:HA}/Scer\GAL4^twi.2PE partially rescues pbl³

(van Impel et al., 2009)

Scer\GAL4^twi.2PE/shd^UAS.cPa partially rescues shd¹/shd²

(Petryk et al., 2003)

Fails to rescue

pbl^{ΔBRCT.UAS.Tag:MYC}/Scer\GAL4^twi.2PE fails to rescue pbl³

(van Impel et al., 2009)

Scer\GAL4^twi.2PE/pbl^{ΔN-term.UAS.Tag:HA} fails to rescue pbl³

(van Impel et al., 2009)

Scer\GAL4^twi.2PE/pbl^{DH.UAS.Tag:HA} fails to rescue pbl³

(van Impel et al., 2009)

pbl^{ΔC-term.UAS.Tag:HA}/Scer\GAL4^twi.2PE fails to rescue pbl³

(van Impel et al., 2009)

Scer\GAL4^twi.2PE/pbl^{DH-PH-V531D.UAS.Tag:HA} fails to rescue pbl³

(van Impel et al., 2009)

Scer\GAL4^twi.2PE/wit^UAS.cMa fails to rescue wit^unspecified

(Marques et al., 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (9)

Bloomington

2517

y¹ w^*; P{GAL4-twi.2xPE}2

58804

y¹ w^*; P{GAL4-twi.2xPE}3

84311

w^*; P{GAL4-twi.2xPE}1; P{UAS-GC3Ai}3

84290

w^*; P{GAL4-twi.2xPE}1/CyO; P{UAS-CC3Ai}3

84332

w^*; P{GAL4-twi.2xPE}1/CyO; P{UAS-VC3Ai}3

25707

P{UAS-Dcr-2.D}1, w¹¹¹⁸; P{GAL4-twi.2xPE}1

67053

w^*; P{GAL4-twi.2xPE}1/CyO; P{UAS-3xFLAG.dCas9.VPR}attP2

67084

w^*; P{GAL4-twi.2xPE}1/CyO; P{UAS-Cas9.P2}attP2/TM6B, Tb¹

Kyoto

107376

y¹ w^*; P{GAL4-twi.2xPE}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

GAL4^twi.2PE

Scer\GAL4^twi.2PE

Name Synonyms

Secondary FlyBase IDs

References (38)

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