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Citation
Schwarz, B., Hollfelder, D., Scharf, K., Hartmann, L., Reim, I. (2018). Diversification of heart progenitor cells by EGF signaling and differential modulation of ETS protein activity.  eLife 7(): e32847.
FlyBase ID
FBrf0239405
Publication Type
Research paper
Abstract

For coordinated circulation, vertebrate and invertebrate hearts require stereotyped arrangements of diverse cell populations. This study explores the process of cardiac cell diversification in the Drosophila heart, focusing on the two major cardioblast subpopulations: generic working myocardial cells and inflow valve-forming ostial cardioblasts. By screening a large collection of randomly induced mutants, we identified several genes involved in cardiac patterning. Further analysis revealed an unexpected, specific requirement of EGF signaling for the specification of generic cardioblasts and a subset of pericardial cells. We demonstrate that the Tbx20 ortholog Midline acts as a direct target of the EGFR effector Pointed to repress ostial fates. Furthermore, we identified Edl/Mae, an antagonist of the ETS factor Pointed, as a novel cardiac regulator crucial for ostial cardioblast specification. Combining these findings, we propose a regulatory model in which the balance between activation of Pointed and its inhibition by Edl controls cardioblast subtype-specific gene expression.

PubMed ID
PubMed Central ID
PMC6033539 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    eLife
    Title
    eLife
    ISBN/ISSN
    2050-084X
    Data From Reference
    Aberrations (13)
    Alleles (59)
    Gene Groups (1)
    Genes (32)
    Natural transposons (1)
    Insertions (8)
    Experimental Tools (2)
    Transgenic Constructs (20)