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General Information
Symbol
Dmel\twiUAS.cBa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Baylies
FlyBase ID
FBal0050680
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-twi, UAS-twist
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Expression of a full length twi cDNA is governed by UASt regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The gl-positive corpora cardiaca precursor cells are missing in stage 12 embryos expressing twiScer\UAS.cBa under the control of Scer\GAL4twi.2PE.

Developing indirect flight muscles degenerate in animals expressing twiScer\UAS.cBa under the control of Scer\GAL41151.

When twiScer\UAS.cBa is driven by Scer\GAL4twi.PB mutant embryos exhibit a near complete loss of cluster I slou expressing muscle founder cells. Cluster II cells are unaffected.

There are no dorsal-ventral indirect flight muscle founder myoblasts in twiScer\UAS.cBa; Scer\GAL41151 pupae at 12 hours after puparium formation.

Over expression of twiScer\UAS.cBa when driven by Scer\GAL4twi.PB converts non-somatic mesoderm into somatic muscle. Ectopic multinucleated somatic muscle cells are detected dorsally where the heart normally forms and around the gut and central nervous system. These muscles do not spread out and form a pattern owing to lack of epidermis.

Expression of twiScer\UAS.cBa under the control of Scer\GAL4twi.PG leads to occasional extra eve-expressing progenitor cells in the mesoderm.

The larval templates of the indirect flight muscles do not split in animals expressing twiScer\UAS.cBa under the control of Scer\GAL41151 and the indirect flight muscles degenerate completely. Direct flight muscles are present and appear normal.

When expression is driven by Scer\GAL4how-24B somatic myogenesis and segregation of twi expressing adult myoblasts occur normally. With extra copies of P{UAS-twi.B} some somatic muscle patterning defects do occur and minor defects in the differentiation of visceral mesoderm (e.g. gastric caecae) and heart occur. When expression is driven by Scer\GAL4twi.PB, the somatic muscles develop normally but the development of the visceral mesoderm and heart are disturbed. The number of visceral mesoderm progenitors was reduced and the cardial and pericardial cells were missing or deranged. Somatic muscle-like structures form on the gut and heart. When expression is driven by Scer\GAL4da.G32 ectodermal derivatives fail to develop. External (normally epidermal) cells express myosin and fuse to form bi- or tri-nucleate syncytia.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Suppressed by
Enhancer of
Suppressor of
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

The number of extra eve-expressing progenitor cells in the mesoderm is substantially increased when twiScer\UAS.cBa is co-expressed with wgScer\UAS.cGa under the control of Scer\GAL4twi.PG. Co-expression of twiScer\UAS.cBa and Ras85DG13Q.Scer\UAS under the control of Scer\GAL4twi.PG results in a lateral expansion of the eve-expressing progenitor clusters in the mesoderm. Co-expression of twiScer\UAS.cBa, Ras85DG13Q.Scer\UAS and wgScer\UAS.cGa under the control of Scer\GAL4twi.PG results in an almost continuous anteroposterior stripe of eve-expressing progenitor cells confined to the dorsal mesoderm.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

twiScer\UAS.cBa completely rescues all phenotypes seen in twi1/twi1. The addition of da5/+ to twiScer\UAS.cBa, Scer\GAL4twi.PB embryos enhances the losses in gut and heart forming mesoderm. Two copies can also enhance the ectopic muscle phenotype as well.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
twiScer\UAS.cBa
twiUAS.cBa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Baylies
Secondary FlyBase IDs
    References (19)