FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\UbxUAS.gRNAx4.pCFD6
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General Information
Symbol
Dmel\UbxUAS.gRNAx4.pCFD6
Species
D. melanogaster
Name
FlyBase ID
FBal0365952
Feature type
allele
Associated gene
Dmel\Ubx
Associated Insertion(s)
Carried in Construct
P{UAS-Ubx.gRNAx4.pCFD6}
Key Links
Transgenic product class
sgRNA
(Domsch et al., 2021)
Mutagen
Nature of the Allele
Transgenic product class
sgRNA
(Domsch et al., 2021)
Mutagen
in vitro construct
(Domsch et al., 2021)
Progenitor genotype
Carried in construct
P{UAS-Ubx.gRNAx4.pCFD6}
Cytology
Description

UAS regulatory sequences drive expression of four sgRNAs that target Ubx.

(Domsch et al., 2021)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Ubx
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      CRISPR/Cas9-mediated mutagenesis of Ubx by the co-expression of UbxUAS.gRNAx4.pCFD6 and Spyo\Cas9UAS.P2 under the combined control of Scer\GAL4Mef2.247 and Scer\GAL4twi.2PE leads to stage 16 embryos with morphological defects, as muscles are malformed, detached, or missing.

      defects in morphology, as muscles were malformed, detached, or missing

      (Domsch et al., 2021)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      UbxUAS.gRNAx4.pCFD6
      Name Synonyms
      Secondary FlyBase IDs
        References (1)