FlyBase Allele Report: Dmel\Ret[GD45]

General Information

Symbol

Dmel\Ret^GD45

Species

D. melanogaster

Name

FlyBase ID

FBal0208260

Feature type

allele

Associated gene

Dmel\Ret

Associated Insertion(s)

Carried in Construct

P{GD45}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD45}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD45

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased occurrence of cell division | adult stage, with Scer\GAL4^NP7397

(Perea et al., 2017)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

intestinal stem cell of posterior adult midgut epithelium, with Scer\GAL4^NP7397

(Perea et al., 2017)

Detailed Description

Statement

Reference

Adult flies expressing Ret^GD45 (and UAS-Dicer2 to enhance RNAi efficiency) driven by Scer\GAL4^esg-NP7397 combined with tub-Gal80[ts] (for temporal control) display significant decrease in midgut stem cell proliferation (assessed by number of pH3-positive cells) both during normal homeostasis and ageing and during chemical damage-induced regeneration. However, the number of stem cell per midgut as well as the ratios of their differentiated progeny are comparable to those observed in wild-type flies.

(Perea et al., 2017)

Adults expressing Ret^GD45 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of Ret^GD45 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or partial lethality, depending on the particular P{GD45} insertion line used.

(Neely et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Ret^GD45, Scer\GAL4^NP7397 is a suppressor of increased occurrence of cell division | adult stage phenotype of Scer\GAL4^NP7397, wg^{UAS.cHa.Tag:HA}

(Perea et al., 2017)

Ret^GD45, Scer\GAL4^NP7397 is a suppressor of increased occurrence of cell division | adult stage phenotype of Scer\GAL4^NP7397, sgg^A81T.UAS

(Perea et al., 2017)

Ret^GD45, Scer\GAL4^NP7397 is a suppressor of increased occurrence of cell division | adult stage phenotype of Scer\GAL4^NP7397, Src42A^CA.UAS

(Perea et al., 2017)

NOT Suppressor of

Statement

Reference

Ret^GD45, Scer\GAL4^NP7397 is a non-suppressor of increased occurrence of cell division | adult stage phenotype of Scer\GAL4^NP7397, spi^s.UAS

(Perea et al., 2017)

Ret^GD45, Scer\GAL4^NP7397 is a non-suppressor of increased occurrence of cell division | adult stage phenotype of Scer\GAL4^NP7397, upd1^UAS.cUa

(Perea et al., 2017)

Phenotype Manifest In

Suppressor of

Statement

Reference

Ret^GD45, Scer\GAL4^NP7397 is a suppressor of intestinal stem cell of posterior adult midgut epithelium phenotype of Scer\GAL4^NP7397, wg^{UAS.cHa.Tag:HA}

(Perea et al., 2017)

Ret^GD45, Scer\GAL4^NP7397 is a suppressor of intestinal stem cell of posterior adult midgut epithelium phenotype of Scer\GAL4^NP7397, sgg^A81T.UAS

(Perea et al., 2017)

Ret^GD45, Scer\GAL4^NP7397 is a suppressor of intestinal stem cell of posterior adult midgut epithelium phenotype of Scer\GAL4^NP7397, Src42A^CA.UAS

(Perea et al., 2017)

NOT Suppressor of

Statement

Reference

Ret^GD45, Scer\GAL4^NP7397 is a non-suppressor of intestinal stem cell of posterior adult midgut epithelium phenotype of Scer\GAL4^NP7397, spi^s.UAS

(Perea et al., 2017)

Ret^GD45, Scer\GAL4^NP7397 is a non-suppressor of intestinal stem cell of posterior adult midgut epithelium phenotype of Scer\GAL4^NP7397, upd1^UAS.cUa

(Perea et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Ret^GD45 can suppress the increased number of intestinal progenitor cells in mitosis characteristic for adult flies expressing any of the following: wg^{Scer\UAS.cHa.T:Ivir\HA1}, sgg^{A81T.Scer\UAS} or Src42A^CA.Scer\UAS driven by Scer\GAL4^esg-NP7397 (combined with tub-Gal80[ts] for temporal control) but fails to prevent the increased proliferation induced by expression of either upd1^Scer\UAS.cUa or spi^s.Scer\UAS.

(Perea et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v843

w¹¹¹⁸; P{GD45}v843

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Ret^GD45

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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