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General Information
Symbol
Dmel\upd1UAS.cUa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Unknown
FlyBase ID
FBal0268408
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-upd, UAS-upd1
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of upd1.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Adulthood-only expression of upd1UAS.cUa under the control of Scer\GAL4NP5130 (and GAL80[ts] for the temporal control of expression) induces an increase in the number of progenitor cells in the midgut, as well as an increase in mitotic index.

Expression of upd1UAS.cUa under the control of Scer\GAL4rn-GAL4-5 (with tub-Gal80[ts] restricting expression to third instar larvae) does not lead to ectopic wing pouches in wing discs or ectopic wings when compared to controls.

Expression of upd1UAS.cUa under the control of Scer\GAL4GMR15B03 or Scer\GAL4dpp.blk1 leads to disruption of thorax and loss of many mechanosensory chaetas when compared to control adults.

Adult flies expressing upd1Scer\UAS.cUa driven by Scer\GAL4esg-NP7397 combined with tub-Gal80[ts] (for temporal control) display significant increase in midgut stem cell proliferation (assessed by number of pH3-positive cells).

The expression of upd1Scer\UAS.cUa under the control of Scer\GAL4ptc.PU leads to a significant decrease in the size of mesonotum primordia size, from which ectopic wing primordium structures frequently emerge, as compared to controls.

Expression of upd1Scer\UAS.cUa under the control of Scer\GAL4esg-NP5130 (in combination with a Gal80[ts] transgene to restrict expression to the adult stage) results in a significant increase in the number of mitotic cells in the midgut, as compared to controls.

Scer\GAL4ptc-559.1-mediated expression of upd1Scer\UAS.cUa results in overgrown wing discs.

Expression of upd1Scer\UAS.cUa under the control of Scer\GAL4tim.PE results in arrhythmic locomotor activity under constant darkness conditions.

Expression of upd1Scer\UAS.cUa under the control of Scer\GAL4elav.Switch.PO in the presence of RU486 results in defects in locomotor activity rhythms (only 33.3% of flies show rhythmic behaviour under constant darkness conditions).

Expression of upd1Scer\UAS.cUa under the control of Scer\GAL4esg-NP7397 and Scer\GAL80ts.αTub84B induces intestinal stem cell proliferation.

No tumors are seen when upd1Scer\UAS.cUa is expressed in eye-antennal clones under the control of Scer\GAL4Act.PU. Inhibition of apoptosis in upd1Scer\UAS.cUa-expressing cells does not induce tumors.

Wing discs expressing upd1Scer\UAS.cUa under the control of Scer\GAL4Bx-MS1096 show an expansion of the disc epithelium and increased epithelial folding.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Statement
Reference
Suppressed by
NOT suppressed by
Enhancer of
Suppressor of
Other
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Statement
Reference

CtBP334Δ4, Scer\GAL4rn-GAL4-5, egrUAS.cUa, upd1UAS.cUa has wing | ectopic phenotype, non-enhanceable by upd2Δ/upd2[+]/upd3Δ/upd3[+]

Suppressed by
NOT suppressed by
Enhancer of
Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of upd1UAS.cUa and egrUAS.cUa under the control of Scer\GAL4rn-GAL4-5 (with tub-Gal80[ts] restricting expression to third instar larvae) leads to ectopic wings compared to controls.

Expression of upd1UAS.cUa under the control of Scer\GAL4rn-GAL4-5 (with tub-Gal80[ts] restricting expression to third instar larvae) in CtBP87De-10/+ background does not lead to ectopic wing pouches in wing discs or ectopic wings when compared to controls.

The increased number of intestinal progenitor cells in mitosis characteristic for adult flies expressing upd1Scer\UAS.cUa driven by Scer\GAL4esg-NP7397 (combined with tub-Gal80[ts] for temporal control) is not suppressed by co-expression of RetGD45.

Co-expression of bunGD1392 suppresses the increased number of mitotic cells seen in the midguts of flies expressing upd1Scer\UAS.cUa under the control of Scer\GAL4esg-NP5130.

Intestinal stem cell proliferation found in flies expressing upd1Scer\UAS.cUa under the control of Scer\GAL4esg-NP7397 and Scer\GAL80ts.αTub84B is completely inhibited when Egfr or Ras85D is down-regulated (through expression of either EgfrdsRNA.Scer\UAS.cUa or Ras85DdsRNA.Scer\UAS.cUa under the control of Scer\GAL4esg-NP7397 and Scer\GAL80ts.αTub84B).

Intestinal stem cell proliferation found in flies expressing upd1Scer\UAS.cUa under the control of Scer\GAL4esg-NP7397 and Scer\GAL80ts.αTub84B is completely inhibited in Ras85DΔC40B mutant clones.

Co-expression of upd1Scer\UAS.cUa and spis.Scer\UAS in intestinal stem cells, under the control of Scer\GAL4Myo31DF-NP0001 and Scer\GAL80ts.αTub84B induces a much higher intestinal stem cell mitotic index than that induced by the activation of either transgene alone.

No tumors are seen when upd1Scer\UAS.cUa is expressed under the control of Scer\GAL4Act.PU in scrib1 mutant eye-antennal clones.

No tumors are seen when upd1Scer\UAS.cUa is expressed under the control of Scer\GAL4Act.PU in wild type eye-antennal disc cells that are adjacent to scrib1 mutant cells.

Eye-antennal disc clones that simultaneously express Ras85DV12.Scer\UAS and upd1Scer\UAS.cUa under the control of Scer\GAL4Act.PU grow into large invasive tumors.

Eye-antennal disc clones that simultaneously express Ras85DV12.Scer\UAS, upd1Scer\UAS.cUa and upd2Scer\UAS.cUa under the control of Scer\GAL4Act.PU grow into large invasive tumors that are larger than those seen when Ras85DV12.Scer\UAS is co-expressed with either upd1Scer\UAS.cUa or upd2Scer\UAS.cUa alone.

Expression of bskDN.Scer\UAS does not suppress the tumors seen in eye-antennal disc clones that simultaneously express Ras85DV12.Scer\UAS and upd1Scer\UAS.cUa under the control of Scer\GAL4Act.PU.

Expression of domeΔCYT.Scer\UAS in eye-antennal disc clones that express Ras85DV12.Scer\UAS and upd1Scer\UAS.cUa under the control of Scer\GAL4Act.PU suppresses the overgrowth and invasion of the nerve cord.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

The increased attraction to food cues, increased food consumption and higher body weight of adult flies expressing upd1JF03149 under the control of Scer\GAL4elav.PU can be rescued by expression of upd1Scer\UAS.cUa.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
osScer\UAS.cUa
upd1Scer\UAS.cUa
upd1UAS.cUa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Unknown
Secondary FlyBase IDs
    References (22)