Expression of upd1UAS.cUa under the control of Scer\GAL4rn-GAL4-5 (with tub-Gal80[ts] restricting expression to third instar larvae) in CtBP87De-10/+ background does not lead to ectopic wing pouches in wing discs or ectopic wings when compared to controls.
The increased number of intestinal progenitor cells in mitosis characteristic for adult flies expressing upd1Scer\UAS.cUa driven by Scer\GAL4esg-NP7397 (combined with tub-Gal80[ts] for temporal control) is not suppressed by co-expression of RetGD45.
Intestinal stem cell proliferation found in flies expressing upd1Scer\UAS.cUa under the control of Scer\GAL4esg-NP7397 and Scer\GAL80ts.αTub84B is completely inhibited when Egfr or Ras85D is down-regulated (through expression of either EgfrdsRNA.Scer\UAS.cUa or Ras85DdsRNA.Scer\UAS.cUa under the control of Scer\GAL4esg-NP7397 and Scer\GAL80ts.αTub84B).
Co-expression of upd1Scer\UAS.cUa and spis.Scer\UAS in intestinal stem cells, under the control of Scer\GAL4Myo31DF-NP0001 and Scer\GAL80ts.αTub84B induces a much higher intestinal stem cell mitotic index than that induced by the activation of either transgene alone.
Eye-antennal disc clones that simultaneously express Ras85DV12.Scer\UAS, upd1Scer\UAS.cUa and upd2Scer\UAS.cUa under the control of Scer\GAL4Act.PU grow into large invasive tumors that are larger than those seen when Ras85DV12.Scer\UAS is co-expressed with either upd1Scer\UAS.cUa or upd2Scer\UAS.cUa alone.
Expression of domeΔCYT.Scer\UAS in eye-antennal disc clones that express Ras85DV12.Scer\UAS and upd1Scer\UAS.cUa under the control of Scer\GAL4Act.PU suppresses the overgrowth and invasion of the nerve cord.