FlyBase Allele Report: Dmel\upd2[UAS.cUa]

General Information

Symbol

Dmel\upd2^UAS.cUa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Unknown

FlyBase ID

FBal0316452

Feature type

allele

Associated gene

Dmel\upd2

Associated Insertion(s)

Carried in Construct

P{UAS-upd2.U}

Also Known As

UAS-upd2

Key Links

Transgenic product class

wild_type

(FlyBase Curators, 2025-)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(FlyBase Curators, 2025-)

Mutagen

in vitro construct

(FlyBase Curators, 2025-)

Progenitor genotype

Carried in construct

P{UAS-upd2.U}

Cytology

Description

UAS regulatory sequences drive expression of upd2.

(FlyBase Curators, 2025-)

FlyBase curator comment: this entry represents a transgenic construct where the particular construct used cannot be determined.

(FlyBase Curators, 2025-)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

upd2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA with Ras85D^V12.UAS

(Wu et al., 2010)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult anterior midgut epithelium, with Scer\GAL4^NP0001

(Zhai et al., 2015)

adult posterior midgut epithelium, with Scer\GAL4^NP0001

(Zhai et al., 2015)

Detailed Description

Statement

Reference

Expression of upd2^Scer\UAS.cUa in enterocytes under the control of Scer\GAL4^{Myo31DF-NP0001} strongly increased the number of mitotic intestinal stem cells in both the anterior and posterior midgut.

(Zhai et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Ras85D^V12.UAS, Scer\GAL4^Act.PU, upd2^UAS.cUa has neoplasia | somatic clone phenotype, enhanceable by upd2^UAS.cUa

(Wu et al., 2010)

Enhancer of

Statement

Reference

upd2^UAS.cUa is an enhancer of neoplasia | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, upd1^UAS.cUa

(Wu et al., 2010)

upd2^UAS.cUa is an enhancer of neoplasia | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, upd2^UAS.cUa

(Wu et al., 2010)

Other

Statement

Reference

Ras85D^V12.UAS, Scer\GAL4^Act.PU, upd2^UAS.cUa has neoplasia | somatic clone phenotype

(Wu et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Ras85D^V12.UAS, Scer\GAL4^Act.PU, upd2^UAS.cUa has eye disc | somatic clone phenotype, enhanceable by upd2^UAS.cUa

(Wu et al., 2010)

Suppressed by

Statement

Reference

Scer\GAL4^NP0001, upd2^UAS.cUa has adult posterior midgut epithelium phenotype, suppressible by Sox21a⁶

(Zhai et al., 2015)

NOT suppressed by

Statement

Reference

Scer\GAL4^NP0001, upd2^UAS.cUa has adult anterior midgut epithelium phenotype, non-suppressible by Sox21a⁶

(Zhai et al., 2015)

Enhancer of

Statement

Reference

upd2^UAS.cUa is an enhancer of eye disc | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, upd1^UAS.cUa

(Wu et al., 2010)

upd2^UAS.cUa is an enhancer of eye disc | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, upd2^UAS.cUa

(Wu et al., 2010)

Other

Statement

Reference

Ras85D^V12.UAS, Scer\GAL4^Act.PU, upd2^UAS.cUa has eye disc | somatic clone phenotype

(Wu et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

A Sox21a⁶ mutant background suppresses the increase in intestinal stem cells (ISCs) usually seen in the posterior midgut when upd2^Scer\UAS.cUa is expressed in enterocytes under the control of Scer\GAL4^{Myo31DF-NP0001}. An increase in ISCs is only seen in the anterior midgut.

Expression of upd2^HMS00901 in enterocytes under the control of Scer\GAL4^{Myo31DF-NP0001} strongly reduces tumor formation in the anterior midgut of Sox21a⁶ mutant flies.

(Zhai et al., 2015)

Eye-antennal disc clones that simultaneously express Ras85D^V12.Scer\UAS and upd2^Scer\UAS.cUa under the control of Scer\GAL4^Act.PU grow into large invasive tumors.

Eye-antennal disc clones that simultaneously express Ras85D^V12.Scer\UAS, upd1^Scer\UAS.cUa and upd2^Scer\UAS.cUa under the control of Scer\GAL4^Act.PU grow into large invasive tumors that are larger than those seen when Ras85D^V12.Scer\UAS is co-expressed with either upd1^Scer\UAS.cUa or upd2^Scer\UAS.cUa alone.

(Wu et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

upd2^Scer\UAS.cUa

upd2^UAS.cUa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Unknown

Secondary FlyBase IDs

References (7)

Report Sections

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