FlyBase Allele Report: Dmel\Ras85D[RNAi.UAS.cUa]

General Information

Symbol

Dmel\Ras85D^RNAi.UAS.cUa

Species

D. melanogaster

Name

FlyBase ID

FBal0407470

Feature type

allele

Associated gene

Dmel\Ras85D

Associated Insertion(s)

Carried in Construct

P{UAS-Ras85D.RNAi.U}

Also Known As

UAS-Ras85D^RNAi, UAS-Ras RNAi

Key Links

Transgenic product class

RNAi_reagent

(FlyBase Curators, 2025-)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(FlyBase Curators, 2025-)

Mutagen

in vitro construct

(FlyBase Curators, 2025-)

Progenitor genotype

Carried in construct

P{UAS-Ras85D.RNAi.U}

Cytology

Description

UAS regulatory sequences drive expression of a Ras85D RNAi construct.

(FlyBase Curators, 2025-)

FlyBase curator comment: this entry represents a transgenic construct where the particular construct used cannot be determined.

(FlyBase Curators, 2025-)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Ras85D

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult midgut | somatic clone, with Scer\GAL4^NP5130

(Jin et al., 2015)

adult midgut progenitor cell | prepupal stage P1, with Scer\GAL4^NP7397

(Jiang and Edgar, 2009)

embryonic/larval hemocyte | decreased number | third instar larval stage, with Scer\GAL4^Hml.Δ

(Ramond et al., 2020)

fat cell | larval stage, with Scer\GAL4^Hml.Δ

(Ramond et al., 2020)

germarium, with Scer\GAL4^unspecified

(Liu et al., 2010)

intestinal stem cell | adult stage | somatic clone, with Scer\GAL4^NP5130

(Jin et al., 2015)

lipid droplet | larval stage, with Scer\GAL4^Hml.Δ

(Ramond et al., 2020)

spectrosome | increased number, with Scer\GAL4^unspecified

(Liu et al., 2010)

wing vein | absent, with Scer\GAL4^nub.PU

(Reim et al., 2014)

Detailed Description

Statement

Reference

Expression of Ras85D^{dsRNA.UAS.cUa} under the control of Scer\GAL4^Hml.Δ leads to larger lipid droplets and larger fat body cells in larvae.

(Ramond et al., 2020)

Expression of Ras85D^{dsRNA.Scer\UAS.cUa} under the control of Scer\GAL4^esg-NP5130 in intestinal stem cell (ISC) somatic flip-out clones (using tub-Gal80[ts] to limit the time of expression/clone induction) in the adult midgut does not cause any clone overgrowth and the number of mitotic cells per midgut is decreased compared to controls.

(Jin et al., 2015)

Germaria expressing Ras85D^VDRC.cUa driven by Scer\GAL4^unspecified exhibit ectopic spectrosome containing cells.

(Liu et al., 2010)

Expression of Ras85D^{dsRNA.Scer\UAS.cUa} driven by Scer\GAL4^esg-NP7397 in adult midgut progenitor cells (AMPs) significantly decreases both the number and size of AMP clusters compared with control midguts from white prepupae.

(Jiang and Edgar, 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Ras85D^RNAi.UAS.cUa, Scer\GAL4^NP7397 is a suppressor of increased occurrence of cell division | adult stage phenotype of Scer\GAL4^NP7397, upd1^UAS.cUa

(Jiang et al., 2011)

NOT Suppressor of

Statement

Reference

Scer\GAL4^NP5130/Ras85D^RNAi.UAS.cUa is a non-suppressor of increased occurrence of cell division | somatic clone | adult stage phenotype of Scer\GAL4^NP5130, cic^KK100838

(Jin et al., 2015)

Other

Statement

Reference

Ras85D^RNAi.UAS.cUa, Scer\GAL4^nub.PU, amls^GD11667 has visible | adult stage phenotype

(Reim et al., 2014)

Phenotype Manifest In

Suppressor of

Statement

Reference

Ras85D^RNAi.UAS.cUa, Scer\GAL4^NP7397 is a suppressor of intestinal stem cell | adult stage phenotype of Scer\GAL4^NP7397, upd1^UAS.cUa

(Jiang et al., 2011)

NOT Suppressor of

Statement

Reference

Scer\GAL4^NP5130/Ras85D^RNAi.UAS.cUa is a non-suppressor of adult midgut | somatic clone phenotype of Scer\GAL4^NP5130, cic^KK100838

(Jin et al., 2015)

Scer\GAL4^NP5130/Ras85D^RNAi.UAS.cUa is a non-suppressor of intestinal stem cell | somatic clone | adult stage phenotype of Scer\GAL4^NP5130, cic^KK100838

(Jin et al., 2015)

Other

Statement

Reference

Ras85D^RNAi.UAS.cUa, Scer\GAL4^nub.PU, amls^GD11667 has wing margin phenotype

(Reim et al., 2014)

Ras85D^RNAi.UAS.cUa, Scer\GAL4^nub.PU, amls^GD11667 has wing vein | absent phenotype

(Reim et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Intestinal stem cell proliferation found in flies expressing upd1^Scer\UAS.cUa under the control of Scer\GAL4^esg-NP7397 and Scer\GAL80^ts.αTub84B is completely inhibited when Ras85D is down-regulated (through expression of Ras85D^{dsRNA.Scer\UAS.cUa} under the control of Scer\GAL4^esg-NP7397 and Scer\GAL80^ts.αTub84B).

(Jiang et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Ras85D^RNAi.UAS.cUa

Ras85D^VDRC.cUa

Ras85D^{dsRNA.Scer\UAS.cUa}

Ras85D^{dsRNA.UAS.cUa}

Name Synonyms

Secondary FlyBase IDs

FBal0256618
FBal0248865

References (13)

Report Sections

Open Close