FlyBase Allele Report: Dmel\Egfr[RNAi.UAS.cUa]

General Information

Symbol

Dmel\Egfr^RNAi.UAS.cUa

Species

D. melanogaster

Name

FlyBase ID

FBal0407438

Feature type

allele

Associated gene

Dmel\Egfr

Associated Insertion(s)

Carried in Construct

P{UAS-Egfr.RNAi.U}

Also Known As

Egfr RNAi, UAS-Egfr RNAi

Key Links

Transgenic product class

RNAi_reagent

(FlyBase Curators, 2025-)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(FlyBase Curators, 2025-)

Mutagen

in vitro construct

(FlyBase Curators, 2025-)

Progenitor genotype

Carried in construct

P{UAS-Egfr.RNAi.U}

Cytology

Description

UAS regulatory sequences drive expression of an Egfr RNAi construct.

(FlyBase Curators, 2025-)

FlyBase curator comment: this entry represents a transgenic construct where the particular construct used cannot be determined.

(FlyBase Curators, 2025-)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Egfr

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates intestinal cancer

modeled by Src64B^UAS.cUa

(Kohlmaier et al., 2015)

modeled by Src42A^CA.UAS

(Kohlmaier et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult midgut | conditional, with Scer\GAL4^NP7397

(Jiang et al., 2011)

adult midgut | somatic clone, with Scer\GAL4^NP5130

(Jin et al., 2015)

adult midgut progenitor cell | prepupal stage P1, with Scer\GAL4^NP7397

(Jiang and Edgar, 2009)

intestinal stem cell | adult stage | somatic clone, with Scer\GAL4^NP5130

(Jin et al., 2015)

Detailed Description

Statement

Reference

Larvae expressing Egfr^{dsRNA.UAS.cUa} under the control of Scer\GAL4^ChAT.PU show decreased head-touch response.

(Zhang et al., 2020)

Expression of Egfr^{dsRNA.Scer\UAS.cUa} under the control of Scer\GAL4^esg-NP5130 in intestinal stem cell (ISC) somatic flip-out clones (using tub-Gal80[ts] to limit the time of expression/clone induction) in the adult midgut does not cause any clone overgrowth and the number of mitotic cells per midgut is decreased compared to controls.

(Jin et al., 2015)

Expression of Egfr^{dsRNA.Scer\UAS.cUa} in the midgut under the control of Scer\GAL4^esg-NP7397 completely inhibits midgut renewal and proliferation after Pseudomonas entomophila infection. Prolonged Egfr suppression in healthy animals (4 week)s leads to an almost complete loss of enteroblasts and an approximate 33% reduction of intestinal stem cells. In the short term however, Egfr suppression does not significantly alter the number of intestinal stem cells. Old enterocytes, generated before the induction of lineage marking are still present in aged midguts (~1 month), suggesting that enterocyte loss is also partially inhibited.

(Jiang et al., 2011)

Similarly to wild-type, honey bee royal jelly supplemented diet leads to increased body size and shortened developmental time in Scer\GAL4^P0206>Egfr^{dsRNA.Scer\UAS.cUa} and Scer\GAL4^Aug21>Egfr^{dsRNA.Scer\UAS.cUa} flies. Royal jelly has no effect on body size or developmental time in Scer\GAL4^ppl.PP>Egfr^{dsRNA.Scer\UAS.cUa} flies. Similar results are observed in Scer\GAL4^ppl.PP>Egfr^{dsRNA.Scer\UAS.cUa} animals reared on a royalactin supplemented diet.

The increase in cell size in wild-type flies reared on royal jelly-supplemented medium is not observed in transgenic flies expressing Scer\GAL4^ppl.PP>Egfr^{dsRNA.Scer\UAS.cUa}.

(Kamakura, 2011)

Expression of Egfr^{dsRNA.Scer\UAS.cUa} driven by Scer\GAL4^esg-NP7397 in adult midgut progenitor cells (AMPs) significantly decreases both the number and size of AMP clusters compared with control midguts from white prepupae.

(Jiang and Edgar, 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Egfr^RNAi.UAS.cUa, Scer\GAL4^NP7397 is a suppressor of increased occurrence of cell division | adult stage phenotype of Scer\GAL4^NP7397, Src64B^UAS.cUa

(Kohlmaier et al., 2015)

Egfr^RNAi.UAS.cUa, Scer\GAL4^NP7397 is a suppressor of increased occurrence of cell division | adult stage phenotype of Scer\GAL4^NP7397, Src42A^UAS.cPa

(Kohlmaier et al., 2015)

Egfr^RNAi.UAS.cUa, Scer\GAL4^NP7397 is a suppressor of increased occurrence of cell division | adult stage phenotype of Scer\GAL4^NP7397, upd1^UAS.cUa

(Jiang et al., 2011)

Egfr^RNAi.UAS.cUa, Scer\GAL4^rho.PU is a suppressor of increased body size phenotype of Amel\MRJP1^UAS.cKa, Scer\GAL4^rho.PU

(Kamakura, 2011)

Egfr^RNAi.UAS.cUa, Scer\GAL4^rho.PU is a suppressor of long lived | female limited phenotype of Amel\MRJP1^UAS.cKa, Scer\GAL4^rho.PU

(Kamakura, 2011)

Egfr^RNAi.UAS.cUa, Scer\GAL4^ppl.PP is a suppressor of increased body size phenotype of Amel\MRJP1^UAS.cKa, Scer\GAL4^ppl.PP

(Kamakura, 2011)

Egfr^RNAi.UAS.cUa/Scer\GAL4^rho.PU is a suppressor of long lived | female limited phenotype of Amel\MRJP1^UAS.cKa, Scer\GAL4^ppl.PP

(Kamakura, 2011)

NOT Suppressor of

Statement

Reference

Egfr^RNAi.UAS.cUa, Scer\GAL4^NP5130 is a non-suppressor of increased occurrence of cell division | somatic clone | adult stage phenotype of Scer\GAL4^NP5130, cic^KK100838

(Jin et al., 2015)

Phenotype Manifest In

Suppressor of

Statement

Reference

Egfr^RNAi.UAS.cUa, Scer\GAL4^NP7397 is a suppressor of adult midgut phenotype of Scer\GAL4^NP7397, Src42A^UAS.cPa

(Kohlmaier et al., 2015)

Egfr^RNAi.UAS.cUa, Scer\GAL4^NP7397 is a suppressor of adult midgut phenotype of Scer\GAL4^NP7397, Src64B^UAS.cUa

(Kohlmaier et al., 2015)

Egfr^RNAi.UAS.cUa, Scer\GAL4^NP7397 is a suppressor of intestinal stem cell | adult stage phenotype of Scer\GAL4^NP7397, upd1^UAS.cUa

(Jiang et al., 2011)

NOT Suppressor of

Statement

Reference

Egfr^RNAi.UAS.cUa, Scer\GAL4^NP5130 is a non-suppressor of adult midgut | somatic clone phenotype of Scer\GAL4^NP5130, cic^KK100838

(Jin et al., 2015)

Egfr^RNAi.UAS.cUa, Scer\GAL4^NP5130 is a non-suppressor of intestinal stem cell | somatic clone | adult stage phenotype of Scer\GAL4^NP5130, cic^KK100838

(Jin et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The overgrowth and increased mitotic rate of intestinal stem cell somatic flip-out clones expressing cic^KK100838 under the control of Scer\GAL4^esg-NP5130 (using tub-Gal80[ts] to limit the time of expression/clone induction) in the adult midgut cannot be suppressed by co-expression of Egfr^{dsRNA.Scer\UAS.cUa}.

(Jin et al., 2015)

Intestinal stem cell proliferation found in flies expressing upd1^Scer\UAS.cUa under the control of Scer\GAL4^esg-NP7397 and Scer\GAL80^ts.αTub84B is completely inhibited when Egfr or Ras85D is down-regulated (through expression of either Egfr^{dsRNA.Scer\UAS.cUa} or Ras85D^{dsRNA.Scer\UAS.cUa} under the control of Scer\GAL4^esg-NP7397 and Scer\GAL80^ts.αTub84B).

(Jiang et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Egfr^RNAi.UAS.cUa

Egfr^VDRC.cUa

Egfr^{dsRNA.Scer\UAS.cUa}

Egfr^{dsRNA.UAS.cUa}

Name Synonyms

Secondary FlyBase IDs

FBal0256619
FBal0268506

References (11)

Report Sections

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