FlyBase Recombinant Construct Report: P{GAL4-sim.3.7}

General Information

Symbol

P{GAL4-sim.3.7}

FlyBase ID

FBtp0007877

Feature type

transgenic_transposable_element

Component Allele(s)

Scer\GAL4^sim.P3.7

Also Known As

sim-Gal4, simGAL4, P[sim-Gal4], 3.7sim-Gal4, single-minded (sim)-Gal4, simG4

Size

Expression Data

GAL4 reporter/driver

Associated insertion(s)

3 available

Molecular map

Description and Uses

Construct components

Component

Use(s)

Marker(s)

w^+mC

Component allele Scer\GAL4^sim.P3.7

Transgenic product class

Component

Use(s)

Regulatory region(s)

sim

Encoded product / tool

GAL4

binary expression system - driver

Description

A 3.7kb BamHI fragment of sim 5' flanking DNA containing the early sim promoter and 5' flanking sequences (encodes 18 amino acids of coding sequence) regulates expression of a GAL4 driver.

(Xiao et al., 1996)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Scer\GAL4^sim.P3.7

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

embryonic midline of ventral nerve cord

(Bloomington Drosophila Stock Center, 2017)

embryonic stage 10 -- 17

larval ventral nerve cord | precursor

(Xiao et al., 1996)

ventral midline of embryo

(Xiao et al., 1996)

embryonic stage 11

ventral midline of embryo

Comment: all cells

(Wheeler et al., 2006)

embryonic stage 11 -- 12

ventral midline of embryo

(Fontana and Crews, 2012)

embryonic stage 13

ventral midline of embryo

Comment: all cells

(Wheeler et al., 2006)

embryonic stage 16

ventral midline of embryo

(Fontana and Crews, 2012)

embryonic stage 17

ventral midline of embryo

Comment: all cells

(Wheeler et al., 2006)

Additional Information

Statement

Reference

Drives expression in cells of the ventral midline of the embryonic CNS.

(FlyBase Curators, 2017)

Midline expression is present in all midline cells from segments S1 to A8/9.

(Fontana and Crews, 2012)

Expression assayed at stages 9, 11, 13, and 17. Expression may be continuous between assayed stages in some tissues.

(Wheeler et al., 2006)

In embryos, expression specific to CNS midline precursor cells (CMP); not expressed in ventral ectodermal cells.

(Xiao et al., 1996)

Marker for

ventral midline of embryo

(Xiao et al., 1996)

Reflects expression of

sim

(Bloomington Drosophila Stock Center, 2017, Wheeler et al., 2006, Xiao et al., 1996)

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (2)

Stocks containing insertion(s) of this recombinant construct

Bloomington

26784

w^*; P{GAL4-sim.3.7}2, P{UAS-GFP.btau}2

9150

w^*; P{GAL4-sim.3.7}2/CyO; P{GAL4-sim.3.7}3

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (22)

Reported As

Symbol Synonym

3.7sim-Gal4

(Fontana and Crews, 2012, Freer et al., 2011)

P^simGAL4

(Lanoue and Jacobs, 1999)

P[sim GAL4]

(Sonnenfeld et al., 2005)

P[sim-GAL4]

(Morozova et al., 2010, Sedaghat et al., 2002, Battye et al., 1999)

P[sim-Gal4]

(Sonnenfeld et al., 2004)

P{3.7sim-Gal4}

(FlyBase, 1996-)

P{GAL4-sim.3.7}

(Ostrowski et al., 2008, FlyBase, 1996-)

Sim-Gal4

(Ismail et al., 2019)

Sim:Gal4

(Sears et al., 2003)

Single-minded GAL4

(Moyer and Jacobs, 2008)

p[sim-Gal4]

(Sedaghat and Sonnenfeld, 2002)

sim Gal4

(Ostrowski et al., 2008)

sim-3.7-Gal4

(Pearson and Crews, 2014)

sim-GAL4

(Gilbert et al., 2024, Banerjee et al., 2017, Alavi et al., 2016, Newquist et al., 2013, Newquist et al., 2013, Shen et al., 2013, Tio et al., 2011, Zhang et al., 2011, Crowner et al., 2003, Georgiou and Tear, 2003, Giniger, 2003.7.28, Georgiou and Tear, 2002, Kramer et al., 2001, Kramer et al., 2001, Lanoue et al., 2000)

sim-Gal4

(Banisch et al., 2021, Babski et al., 2019, Gordon et al., 2018, Wheeler et al., 2012, Stagg et al., 2011, Watson et al., 2011, Kadam et al., 2009, Ostrowski et al., 2008, Wheeler et al., 2006, Orgogozo et al., 2004)

sim.Gal4

(Kadam et al., 2012)

sim3.7-Gal4

(Pearson et al., 2016)

simG4

(Liu et al., 2024, Chanana et al., 2009)

simGAL4

(Newquist et al., 2013, Shen et al., 2013, Garbe et al., 2007, Settle et al., 2003)

simGal4

(Schachtner et al., 2015, Butí et al., 2014)

single-minded (sim)-GAL4

(Morozova et al., 2010)

single-minded (sim)-Gal4

(Sonnenfeld et al., 2010)

Secondary FlyBase IDs

References (52)

Report Sections

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