FlyBase Allele Report: Mmus\Sox2[EnR.UAS.Tag:MYC]

General Information

Symbol

Mmus\Sox2^{EnR.UAS.Tag:MYC}

Species

M. musculus

Name

FlyBase ID

FBal0291189

Feature type

allele

Associated gene

Mmus\Sox2

Associated Insertion(s)

Carried in Construct

P{UAS-mSox2.EnRep}

Transgenic product class

dominant_negative_variant

(Shen et al., 2013)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Shen et al., 2013)

Mutagen

in vitro construct

(Shen et al., 2013)

Progenitor genotype

Carried in construct

P{UAS-mSox2.EnRep}

Cytology

Description

UAS regulates expression of an N-terminal fragment of Mmus\Sox2, including the HMG domain, fused to the en repressor domain at the C-terminus followed by a Tag:MYC tag. This acts as a dominant negative protein.

(Shen et al., 2013)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mmus\Sox2

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage, with Scer\GAL4^pros.C20

(Shen et al., 2013)

abnormal neuroanatomy | embryonic stage, with Scer\GAL4^sim.P3.7

(Shen et al., 2013)

Phenotype Manifest In

larval central nervous system, with Scer\GAL4^pros.C20

(Shen et al., 2013)

larval central nervous system, with Scer\GAL4^sim.P3.7

(Shen et al., 2013)

Detailed Description

Statement

Reference

Scer\GAL4^sim.P3.7- or Scer\GAL4^pros.C20-mediated expression of Mmus\Sox2^{Scer\UAS.T:Rep-en,T:Hsap\MYC} results in 50-70% of embryos exhibiting some degree of CNS disruption.

(Shen et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Mmus\Sox2^{EnR.UAS.Tag:MYC}/Scer\GAL4^sim.P3.7 is an enhancer of abnormal neuroanatomy | embryonic stage phenotype of D^r72/Df(3L)fz-GS1a

(Shen et al., 2013)

Phenotype Manifest In

Enhancer of

Statement

Reference

Mmus\Sox2^{EnR.UAS.Tag:MYC}/Scer\GAL4^sim.P3.7 is an enhancer of larval central nervous system phenotype of D^r72/Df(3L)fz-GS1a

(Shen et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Scer\GAL4^sim.P3.7-mediated expression of Mmus\Sox2^{Scer\UAS.T:Rep-en,T:Hsap\MYC} enhances the embryonic CNS defects of D^r72/Df(3L)fz-GS1a animals.

(Shen et al., 2013)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Mmus\Sox2^{EnR.Scer\UAS.T:Hsap\MYC}

Mmus\Sox2^{EnR.UAS.Tag:MYC}

Mmus\Sox2^{Scer\UAS.T:Rep-en,T:Hsap\MYC}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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