FlyBase Allele Report: Mmmm\ras[N17.UAS]

General Information

Symbol

Mmmm\ras^N17.UAS

Species

m. unknown

Name

FlyBase ID

FBal0050147

Feature type

allele

Associated gene

Mmmm\ras

Associated Insertion(s)

Carried in Construct

P{UAS-ras.N17}

Also Known As

UAS-ras.N17, UAS-ras^N17, UAS-Ras^DN

Transgenic product class

dominant_negative_variant

(Lee et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Lee et al., 1996)

Mutagen

in vitro construct

(Lee et al., 1996)

Progenitor genotype

Carried in construct

P{UAS-ras.N17}

Cytology

Description

UASt regulatory sequences drive expression of a dominant-negative form of Mmmm\ras.

(Lee et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mmmm\ras

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

hypoplasia | somatic clone, with Scer\GAL4^Tub.PU

(Guo et al., 2013)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Mmmm\ras^N17.Scer\UAS (driven by Scer\GAL4^Dll-md23) in type II neuroblast lineage MARCM clones has no significant effect on the clone size or the number of progenitor cells it contains in third instar larvae.

(Rives-Quinto et al., 2017)

Expression of Mmmm\ras^N17.Scer\UAS driven by Scer\GAL4^dpp.PU does not lead to tissue overgrowth in eye-antennal imaginal or wing discs.

(Snee et al., 2016)

Expression of Scer\GAL4^tub.PU>Mmmm\ras^N17.Scer\UAS using the MARCM system dramatically inhibits clone growth in the posterior midgut.

(Guo et al., 2013)

Mmmm\ras^N17.Scer\UAS; Scer\GAL4^{Ubx-lac1-Gal4} adults exhibit wing duplication in the posterior compartment and partial loss of notum and/or hinge. Occasionally pharate adults die within the pupal case, with severe loss of notum tissue.

(Pallavi and Shashidhara, 2003)

All midline glial cells are apoptotic during embryonic stage 14 in animals expressing Mmmm\ras^N17.Scer\UAS under the control of Scer\GAL4^sim.P3.7.

(Settle et al., 2003)

The presence of Scer\GAL4^c306 causes a mild delay in the migration of border cells in egg chambers.

(Lee et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^Dll-md23/Mmmm\ras^N17.UAS is a suppressor of neoplasia | somatic clone | third instar larval stage phenotype of cno^R2, scrib¹

(Rives-Quinto et al., 2017)

Scer\GAL4^Dll-md23/Mmmm\ras^N17.UAS is a suppressor | somatic clone of abnormal neuroanatomy | somatic clone | third instar larval stage phenotype of cno^R2, scrib¹

(Rives-Quinto et al., 2017)

Scer\GAL4^Dll-md23/Mmmm\ras^N17.UAS is a suppressor | somatic clone of abnormal size | somatic clone | third instar larval stage phenotype of cno^R2, scrib¹

(Rives-Quinto et al., 2017)

Scer\GAL4^Dll-md23/Mmmm\ras^N17.UAS is a suppressor | somatic clone of increased cell number | somatic clone | third instar larval stage phenotype of cno^R2, scrib¹

(Rives-Quinto et al., 2017)

NOT Suppressor of

Statement

Reference

Scer\GAL4^repo/Mmmm\ras^N17.UAS is a non-suppressor of abnormal neuroanatomy | third instar larval stage phenotype of AdamTS-A^rnwy2/AdamTS-A^rnwy1

(Skeath et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Mmmm\ras^N17.UAS, Scer\GAL4^org-1.HN39 has adult heart ventral longitudinal muscle cell | P-stage phenotype, suppressible | partially by yki^{S168A.UAS.EGFP,Tag:HA}, Scer\GAL4^org-1.HN39

(Rose et al., 2022)

Suppressor of

Statement

Reference

Scer\GAL4^Dll-md23/Mmmm\ras^N17.UAS is a suppressor | somatic clone of larval brain | somatic clone | third instar larval stage phenotype of cno^R2, scrib¹

(Rives-Quinto et al., 2017)

NOT Suppressor of

Statement

Reference

Scer\GAL4^repo/Mmmm\ras^N17.UAS is a non-suppressor of larval brain | third instar larval stage phenotype of AdamTS-A^rnwy2/AdamTS-A^rnwy1

(Skeath et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The overgrowth and increased number of progenitor cells characteristic for cno^R2;scrib¹ double mutant MARCM clones is significantly suppressed by Scer\GAL4^Dll-md23-driven expression of Mmmm\ras^N17.Scer\UAS in the mutant clones.

(Rives-Quinto et al., 2017)

The neural invasion phenotype (neural cells from the brain invade adjacent peripheral structures such as imaginal discs) observed in AdamTS-A^rnwy1/AdamTS-A^rnwy2 mutant third instar larvae is not significantly suppressed by Scer\GAL4^repo-driven expression of Mmmm\ras^N17.Scer\UAS.

(Skeath et al., 2017)

Co-expression of Dis3^KK101473 and Mmmm\ras^N17.Scer\UAS driven by Scer\GAL4^dpp.PU does not lead to tissue overgrowth in eye-antennal imaginal or wing discs.

(Snee et al., 2016)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

4846

P{UAS-ras.N17}TL1, w¹¹¹⁸

Kyoto

107905

P{UAS-ras.N17}TL1, w¹¹¹⁸

Notes on Origin

Discoverer

Comments

This dominant negative allele works better than the Drosophila allele carried in P{UAS-Ras85D.N17}.

(Montell, 1998.11.25)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Mmmm\ras^N17.Scer\UAS

Mmmm\ras^N17.UAS

mammal\ras^N17.Scer\UAS

mammal\ras^N17.UAS

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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