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General Information
Symbol
Dmel\AdamTS-Arnwy1
Species
D. melanogaster
Name
FlyBase ID
FBal0336689
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Tirant insertion in the most 5' exon of AdamTS-A.

Insertion components
Tirant{}AdamTS-Arnwy1
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Homozygosity in AdamTS-Arnwy1 leads to exodus of hundreds to thousands of cells out of the central nervous system through nerves towards and into peripheral tissues such as eye and leg imaginal discs in third instar larvae, the invading cells include neuroblasts as well as neurons. This invasion phenotype is barely detectable in second instar larvae and becomes fully manifest in the third instar (concomitant with the most rapid phase of the CNS growth) and is associated with 2- to 3-day delay in pupariation. The proliferative index of neuroblast in AdamTS-Arnwy1 larvae is not significantly different from that of wild-type larvae.

AdamTS-Arnwy1/AdamTS-Arnwy2 transheterozygous larvae also show the neural invasion phenotype.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
Phenotype Manifest In
Suppressed by
NOT suppressed by
Additional Comments
Genetic Interactions
Statement
Reference

The neural invasion phenotype (neural cells from the brain invade adjacent peripheral structures such as imaginal discs) observed in AdamTS-Arnwy1/AdamTS-Arnwy2 mutant third instar larvae is partially suppressed by combination with: LanAMI06740, heterozygosity of Df(2L)BSC172 or expression of any of the following: trolUAS.RG (with Scer\GAL4ppl.PP), mysJF02819 or mysHMS00043 (both with Scer\GAL4repo) or vkgHMC02400 (driven by Scer\GAL4αTub84B.PL combined with tub-Gal80[ts] for temporal control). No significant suppression is observed upon combination with any of the following: LanAMI02491, single copy of LanB1KG03456, DgB48, shgR69 or Sdck10215 or expression of mysHMS00043 if driven by Scer\GAL4elav.PU combined with Scer\GAL80repo.PL, LanAJF02908 or LanB1HMS02451 (both with Scer\GAL4αTub84B.PL combined with tub-Gal80[ts] for temporal control).

Xenogenetic Interactions
Statement
Reference

The neural invasion phenotype (neural cells from the brain invade adjacent peripheral structures such as imaginal discs) observed in AdamTS-Arnwy1/AdamTS-Arnwy2 mutant third instar larvae is not significantly suppressed by Scer\GAL4repo-driven expression of Mmmm\rasN17.Scer\UAS.

Complementation and Rescue Data
Fails to complement
Comments

Expression of either AdamTS-AUAS.RB or AdamTS-AUAS.cIa driven by Scer\GAL4elav.PU fails to rescue the neural invasion phenotype (neural cells from the brain invade adjacent peripheral structures such as imaginal discs) characteristic for AdamTS-Arnwy1/AdamTS-Arnwy2 mutant larvae when combined with Scer\GAL80repo.PL, but the phenotype is rescued if the driver is used alone. Scer\GAL4repo-driven expression also rescues the invasion phenotype but replaces it with severe morphological anomalies: an elongated nerve cord and flattened brain lobes. Expression of either AdamTS-AE439A.UAS or AdamTS-AE591A.UAS controlled by Scer\GAL4repo partially rescues the neural invasion phenotype of AdamTS-Arnwy1/AdamTS-Arnwy2 mutants: ~45% of larvae exhibit modest invasion phenotype, but all the larvae show abnormal elongation of the ventral nerve cord.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (2)