FlyBase Allele Report: Dmel\AdamTS-A[rnwy1]

General Information

Symbol

Dmel\AdamTS-A^rnwy1

Species

D. melanogaster

Name

FlyBase ID

FBal0336689

Feature type

allele

Associated gene

Dmel\AdamTS-A

Associated Insertion(s)

Tirant{}AdamTS-A^rnwy1

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Skeath et al., 2017)

Mutagen

ethyl methanesulfonate

(Skeath et al., 2017)

Progenitor genotype

Associated Insertion(s)

Tirant{}AdamTS-A^rnwy1

Cytology

Description

Tirant insertion in the most 5' exon of AdamTS-A.

(Skeath et al., 2017)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:15,476,078..15,476,078 [-]

(Skeath et al., 2017)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygosity in AdamTS-A^rnwy1 leads to exodus of hundreds to thousands of cells out of the central nervous system through nerves towards and into peripheral tissues such as eye and leg imaginal discs in third instar larvae, the invading cells include neuroblasts as well as neurons. This invasion phenotype is barely detectable in second instar larvae and becomes fully manifest in the third instar (concomitant with the most rapid phase of the CNS growth) and is associated with 2- to 3-day delay in pupariation. The proliferative index of neuroblast in AdamTS-A^rnwy1 larvae is not significantly different from that of wild-type larvae.

AdamTS-A^rnwy1/AdamTS-A^rnwy2 transheterozygous larvae also show the neural invasion phenotype.

(Skeath et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

AdamTS-A^rnwy2/AdamTS-A^rnwy1 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by trol^UAS.RG/Scer\GAL4^ppl.PP

(Skeath et al., 2017)

AdamTS-A^rnwy2/AdamTS-A^rnwy1 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by LanA^MI06740/LanA^MI06740

(Skeath et al., 2017)

AdamTS-A^rnwy2/AdamTS-A^rnwy1 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by Df(2L)BSC172/+

(Skeath et al., 2017)

AdamTS-A^rnwy2/AdamTS-A^rnwy1 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by mys^JF02819/Scer\GAL4^repo

(Skeath et al., 2017)

AdamTS-A^rnwy2/AdamTS-A^rnwy1 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^repo/mys^HMS00043

(Skeath et al., 2017)

AdamTS-A^rnwy2/AdamTS-A^rnwy1 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^αTub84B.PL/vkg^HMC02400

(Skeath et al., 2017)

NOT suppressed by

Statement

Reference

AdamTS-A^rnwy2/AdamTS-A^rnwy1 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by LanB1[+]/LanB1^KG03456

(Skeath et al., 2017)

AdamTS-A^rnwy2/AdamTS-A^rnwy1 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Dg^B48/Dg[+]

(Skeath et al., 2017)

AdamTS-A^rnwy2/AdamTS-A^rnwy1 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by shg[+]/shg^R69

(Skeath et al., 2017)

AdamTS-A^rnwy2/AdamTS-A^rnwy1 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Sdc[+]/Sdc^k10215

(Skeath et al., 2017)

AdamTS-A^rnwy2/AdamTS-A^rnwy1 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav.PU/Scer\GAL80^repo.PL/mys^HMS00043

(Skeath et al., 2017)

AdamTS-A^rnwy2/AdamTS-A^rnwy1 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by LanA^JF02908/Scer\GAL4^αTub84B.PL

(Skeath et al., 2017)

AdamTS-A^rnwy2/AdamTS-A^rnwy1 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Scer\GAL4^αTub84B.PL/LanB1^HMS02451

(Skeath et al., 2017)

AdamTS-A^rnwy2/AdamTS-A^rnwy1 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Scer\GAL4^repo/Mmmm\ras^N17.UAS

(Skeath et al., 2017)

AdamTS-A^rnwy2/AdamTS-A^rnwy1 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by LanA^MI02491/LanA^MI02491

(Skeath et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

AdamTS-A^rnwy2/AdamTS-A^rnwy1 has larval brain | third instar larval stage phenotype, suppressible | partially by trol^UAS.RG/Scer\GAL4^ppl.PP

(Skeath et al., 2017)

AdamTS-A^rnwy2/AdamTS-A^rnwy1 has larval brain | third instar larval stage phenotype, suppressible | partially by LanA^MI06740/LanA^MI06740

(Skeath et al., 2017)

AdamTS-A^rnwy2/AdamTS-A^rnwy1 has larval brain | third instar larval stage phenotype, suppressible | partially by Df(2L)BSC172/+

(Skeath et al., 2017)

AdamTS-A^rnwy2/AdamTS-A^rnwy1 has larval brain | third instar larval stage phenotype, suppressible | partially by mys^JF02819/Scer\GAL4^repo

(Skeath et al., 2017)

AdamTS-A^rnwy2/AdamTS-A^rnwy1 has larval brain | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^repo/mys^HMS00043

(Skeath et al., 2017)

AdamTS-A^rnwy2/AdamTS-A^rnwy1 has larval brain | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^αTub84B.PL/vkg^HMC02400

(Skeath et al., 2017)

NOT suppressed by

Statement

Reference

AdamTS-A^rnwy2/AdamTS-A^rnwy1 has larval brain | third instar larval stage phenotype, non-suppressible by LanA^MI02491/LanA^MI02491

(Skeath et al., 2017)

AdamTS-A^rnwy2/AdamTS-A^rnwy1 has larval brain | third instar larval stage phenotype, non-suppressible by LanB1[+]/LanB1^KG03456

(Skeath et al., 2017)

AdamTS-A^rnwy2/AdamTS-A^rnwy1 has larval brain | third instar larval stage phenotype, non-suppressible by Dg^B48/Dg[+]

(Skeath et al., 2017)

AdamTS-A^rnwy2/AdamTS-A^rnwy1 has larval brain | third instar larval stage phenotype, non-suppressible by shg[+]/shg^R69

(Skeath et al., 2017)

AdamTS-A^rnwy2/AdamTS-A^rnwy1 has larval brain | third instar larval stage phenotype, non-suppressible by Sdc[+]/Sdc^k10215

(Skeath et al., 2017)

AdamTS-A^rnwy2/AdamTS-A^rnwy1 has larval brain | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav.PU/Scer\GAL80^repo.PL/mys^HMS00043

(Skeath et al., 2017)

AdamTS-A^rnwy2/AdamTS-A^rnwy1 has larval brain | third instar larval stage phenotype, non-suppressible by LanA^JF02908/Scer\GAL4^αTub84B.PL

(Skeath et al., 2017)

AdamTS-A^rnwy2/AdamTS-A^rnwy1 has larval brain | third instar larval stage phenotype, non-suppressible by Scer\GAL4^αTub84B.PL/LanB1^HMS02451

(Skeath et al., 2017)

AdamTS-A^rnwy2/AdamTS-A^rnwy1 has larval brain | third instar larval stage phenotype, non-suppressible by Scer\GAL4^repo/Mmmm\ras^N17.UAS

(Skeath et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The neural invasion phenotype (neural cells from the brain invade adjacent peripheral structures such as imaginal discs) observed in AdamTS-A^rnwy1/AdamTS-A^rnwy2 mutant third instar larvae is partially suppressed by combination with: LanA^MI06740, heterozygosity of Df(2L)BSC172 or expression of any of the following: trol^UAS.RG (with Scer\GAL4^ppl.PP), mys^JF02819 or mys^HMS00043 (both with Scer\GAL4^repo) or vkg^HMC02400 (driven by Scer\GAL4^αTub84B.PL combined with tub-Gal80[ts] for temporal control). No significant suppression is observed upon combination with any of the following: LanA^MI02491, single copy of LanB1^KG03456, Dg^B48, shg^R69 or Sdc^k10215 or expression of mys^HMS00043 if driven by Scer\GAL4^elav.PU combined with Scer\GAL80^repo.PL, LanA^JF02908 or LanB1^HMS02451 (both with Scer\GAL4^αTub84B.PL combined with tub-Gal80[ts] for temporal control).

(Skeath et al., 2017)

Xenogenetic Interactions

Statement

Reference

(Skeath et al., 2017)

Complementation and Rescue Data

Fails to complement

AdamTS-A^rnwy2

(Skeath et al., 2017)

Rescued by

AdamTS-A^rnwy2/AdamTS-A^rnwy1 is rescued by AdamTS-A^UAS.RB/Scer\GAL4^repo

(Skeath et al., 2017)

AdamTS-A^rnwy2/AdamTS-A^rnwy1 is rescued by Scer\GAL4^elav.PU/AdamTS-A^UAS.RB

(Skeath et al., 2017)

AdamTS-A^rnwy2/AdamTS-A^rnwy1 is rescued by AdamTS-A^UAS.RB/Scer\GAL4^Gli-rL82

(Skeath et al., 2017)

AdamTS-A^rnwy2/AdamTS-A^rnwy1 is rescued by AdamTS-A^UAS.cIa/Scer\GAL4^repo

(Skeath et al., 2017)

AdamTS-A^rnwy2/AdamTS-A^rnwy1 is rescued by AdamTS-A^UAS.cIa/Scer\GAL4^elav.PU

(Skeath et al., 2017)

Partially rescued by

AdamTS-A^rnwy2/AdamTS-A^rnwy1 is partially rescued by Scer\GAL4^repo/AdamTS-A^E591A.UAS

(Skeath et al., 2017)

AdamTS-A^rnwy2/AdamTS-A^rnwy1 is partially rescued by AdamTS-A^E439A.UAS/Scer\GAL4^repo

(Skeath et al., 2017)

Not rescued by

AdamTS-A^rnwy2/AdamTS-A^rnwy1 is not rescued by Scer\GAL4^elav.PU/AdamTS-A^UAS.RB/Scer\GAL80^repo.PL

(Skeath et al., 2017)

AdamTS-A^rnwy2/AdamTS-A^rnwy1 is not rescued by Scer\GAL4^NP577/AdamTS-A^UAS.RB

(Skeath et al., 2017)

AdamTS-A^rnwy2/AdamTS-A^rnwy1 is not rescued by AdamTS-A^UAS.cIa/Scer\GAL4^elav.PU/Scer\GAL80^repo.PL

(Skeath et al., 2017)

Comments

Expression of either AdamTS-A^UAS.RB or AdamTS-A^UAS.cIa driven by Scer\GAL4^elav.PU fails to rescue the neural invasion phenotype (neural cells from the brain invade adjacent peripheral structures such as imaginal discs) characteristic for AdamTS-A^rnwy1/AdamTS-A^rnwy2 mutant larvae when combined with Scer\GAL80^repo.PL, but the phenotype is rescued if the driver is used alone. Scer\GAL4^repo-driven expression also rescues the invasion phenotype but replaces it with severe morphological anomalies: an elongated nerve cord and flattened brain lobes. Expression of either AdamTS-A^E439A.UAS or AdamTS-A^E591A.UAS controlled by Scer\GAL4^repo partially rescues the neural invasion phenotype of AdamTS-A^rnwy1/AdamTS-A^rnwy2 mutants: ~45% of larvae exhibit modest invasion phenotype, but all the larvae show abnormal elongation of the ventral nerve cord.

(Skeath et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

AdamTS-A^rnwy1

(Wittes and Schüpbach, 2019, Skeath et al., 2017)

rnwy¹

(Skeath et al., 2017)

runaway¹

(Skeath et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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