FlyBase Allele Report: Dmel\AdamTS-A[KO]

General Information

Symbol

Dmel\AdamTS-A^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0286141

Feature type

allele

Associated gene

Dmel\AdamTS-A

Associated Insertion(s)

TI{TI}AdamTS-A^KO

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

gene targeting by homologous recombination

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Ismat et al., 2013)

Mutagen

gene targeting by homologous recombination

(Ismat et al., 2013)

Progenitor genotype

Associated Insertion(s)

TI{TI}AdamTS-A^KO

Cytology

Description

Sequence encoding part of the AdamTS-A gene, from the translation start through the cysteine-rich domain, has been deleted and replaced by a w[+] marker gene.

(Ismat et al., 2013)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration (with Df(3R)88F5)

(Ismat et al., 2013)

lethal (with AdamTS-A^rnwy1)

(Skeath et al., 2017)

lethal (with AdamTS-A^rnwy2)

(Skeath et al., 2017)

lethal - all die before end of larval stage (with Df(3R)88F5)

(Skeath et al., 2017)

Phenotype Manifest In

embryonic/larval salivary gland (with Df(3R)88F5)

(Ismat et al., 2013)

embryonic/larval visceral tracheal branch (with Df(3R)88F5)

(Ismat et al., 2013)

germline cell | embryonic stage (with Df(3R)88F5)

(Ismat et al., 2013)

longitudinal trunk visceral muscle primordium (with Df(3R)88F5)

(Ismat et al., 2013)

Detailed Description

Statement

Reference

Approximately 45% of AdamTS-A^KO/Df(3R)88F5 embryos have 25% or more of their germ cells outside the normal germ cell clusters at late embryogenesis (compared to only 6% of wild-type control embryos), indicating defective migration.

Many caudal visceral mesoderm (CVM) cells mis-migrate in AdamTS-A^KO/Df(3R)88F5 embryos, migrating away from the normal two rows of posterior to anterior migrating cells. The mutant embryos show a 40% increase in the number of CVM cells showing mistargeting compared to wild type.

Mis-migration of tracheal visceral branches is seen with a 40% increase compared to wild type in AdamTS-A^KO/Df(3R)88F5 embryos.

Salivary glands of AdamTS-A^KO/Df(3R)88F5 embryos show multiple deformations, including rough and irregular apical surfaces at early stages, and constrictions and bulges at later stages. The apical irregularities are between cells and are always associated with the cell membrane. On average two of the distal-most cells of the salivary gland at stage 12 are longer than wild type, suggesting that the apical surfaces remain attached to the apical extracellular membrane in the mutant embryos as the basal surfaces extend posteriorly during migration.

(Ismat et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

AdamTS-A^KO/Df(3R)88F5 has embryonic/larval salivary gland phenotype, suppressible | partially by Cad99C^120B/Cad99C^57A

(Ismat et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Cad99C^57A/Cad99C^120B significantly rescues the salivary gland defects of AdamTS-A^KO/Df(3R)88F5 embryos.

(Ismat et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

AdamTS-A^KO/Df(3R)88F5 is rescued by AdamTS-A^UAS.cIa/Scer\GAL4^{VP16.nanos.UTR}

(Ismat et al., 2013)

Partially rescued by

AdamTS-A^KO/Df(3R)88F5 is partially rescued by AdamTS-A^UAS.cIa/Scer\GAL4^tey-5053A

(Ismat et al., 2013)

AdamTS-A^KO/Df(3R)88F5 is partially rescued by AdamTS-A^UAS.cIa/Scer\GAL4^btl.PS

(Ismat et al., 2013)

Not rescued by

AdamTS-A^KO/Df(3R)88F5 is not rescued by AdamTS-A^E439A.UAS/Scer\GAL4^tey-5053A

(Ismat et al., 2013)

Comments

Expression of AdamTS-A^Scer\UAS.cIa under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} rescues the germ cell migration defects seen in AdamTS-A^KO/Df(3R)88F5 embryos.

Expression of AdamTS-A^Scer\UAS.cIa under the control of Scer\GAL4^tey-5053A partially rescues the germ cell migration defects seen in AdamTS-A^KO/Df(3R)88F5 embryos.

Expression of AdamTS-A^Scer\UAS.cIa under the control of Scer\GAL4^tey-5053A rescues the caudal visceral mesoderm migration defects seen in AdamTS-A^KO/Df(3R)88F5 embryos.

Expression of AdamTS-A^Scer\UAS.cIa under the control of either Scer\GAL4^tey-5053A or Scer\GAL4^btl.PS rescues the mis-migration of tracheal visceral branches seen in AdamTS-A^KO/Df(3R)88F5 embryos.

Expression of AdamTS-A^{E439A.Scer\UAS} under the control of Scer\GAL4^tey-5053A fails to rescue the caudal visceral mesoderm migration defects seen in AdamTS-A^KO/Df(3R)88F5 embryos.

(Ismat et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

AdamTS-A^KO

(Wittes and Schüpbach, 2019, Skeath et al., 2017, Ismat et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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