FlyBase Allele Report: Dmel\AdamTS-A[UAS.RB]

General Information

Symbol

Dmel\AdamTS-A^UAS.RB

Species

D. melanogaster

Name

FlyBase ID

FBal0336693

Feature type

allele

Associated gene

Dmel\AdamTS-A

Associated Insertion(s)

Carried in Construct

P{UAS-AdamTS-A.RB}

Key Links

Transgenic product class

cDNA

(Skeath et al., 2017)

wild_type

(Skeath et al., 2017)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Skeath et al., 2017)

wild_type

(Skeath et al., 2017)

Mutagen

in vitro construct

(Skeath et al., 2017)

Progenitor genotype

Carried in construct

P{UAS-AdamTS-A.RB}

Cytology

Description

UAS regulatory sequences drive expression of the AdamTS-A-RB isoform (derived from the GH16393 cDNA clone).

(Skeath et al., 2017)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

AdamTS-A

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage, with AdamTS-A^rnwy2/AdamTS-A^rnwy1, Scer\GAL4^repo

(Skeath et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^repo

(Skeath et al., 2017)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of AdamTS-A^UAS.RB under the control of Scer\GAL4^elav.PU (combined with Scer\GAL80^repo.PL) has no effect on larval brain morphology, however expression controlled by Scer\GAL4^repo results in an elongated nerve cord and flattened brain lobes. Similar morphological defects are also observed upon Scer\GAL4^repo-driven expression of AdamTS-A^UAS.RB in the AdamTS-A^rnwy1/AdamTS-A^rnwy2 mutant larvae.

(Skeath et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

AdamTS-A^UAS.RB, Scer\GAL4^repo has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by trol^G0271/trol^G0271

(Skeath et al., 2017)

AdamTS-A^UAS.RB, Scer\GAL4^repo has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by trol^sd/trol^sd

(Skeath et al., 2017)

NOT Suppressor of

Statement

Reference

AdamTS-A^UAS.RB/Scer\GAL4^repo is a non-suppressor of abnormal developmental rate | larval stage phenotype of trol^sd

(Skeath et al., 2017)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

AdamTS-A^UAS.RB, Scer\GAL4^repo has larval ventral nerve cord | third instar larval stage phenotype, non-suppressible by trol^G0271/trol^G0271

(Skeath et al., 2017)

AdamTS-A^UAS.RB, Scer\GAL4^repo has larval ventral nerve cord | third instar larval stage phenotype, non-suppressible by trol^sd/trol^sd

(Skeath et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The abnormal elongation of ventral nerve cord observed in third instar larvae expressing AdamTS-A^UAS.RB under the control of Scer\GAL4^repo is not prevented by homozygosity of trol^G0271 or trol^sd. However, expression of AdamTS-A^UAS.RB under the control of Scer\GAL4^repo does not suppress the larval growth defects characteristic for trol^sd mutants and the larvae still arrest their development at second instar stage.

(Skeath et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

AdamTS-A^UAS.RB/Scer\GAL4^repo rescues AdamTS-A^rnwy2/AdamTS-A^rnwy1

(Skeath et al., 2017)

Scer\GAL4^elav.PU/AdamTS-A^UAS.RB rescues AdamTS-A^rnwy2/AdamTS-A^rnwy1

(Skeath et al., 2017)

AdamTS-A^UAS.RB/Scer\GAL4^Gli-rL82 rescues AdamTS-A^rnwy2/AdamTS-A^rnwy1

(Skeath et al., 2017)

Fails to rescue

Scer\GAL4^elav.PU, AdamTS-A^UAS.RB, Scer\GAL80^repo.PL fails to rescue AdamTS-A^rnwy2/AdamTS-A^rnwy1

(Skeath et al., 2017)

Scer\GAL4^NP577/AdamTS-A^UAS.RB fails to rescue AdamTS-A^rnwy2/AdamTS-A^rnwy1

(Skeath et al., 2017)

Comments

Expression of AdamTS-A^UAS.RB driven by Scer\GAL4^elav.PU fails to rescue the neural invasion phenotype (neural cells from the brain invade adjacent peripheral structures such as imaginal discs) characteristic for AdamTS-A^rnwy1/AdamTS-A^rnwy2 mutant larvae when combined with Scer\GAL80^repo.PL, but the phenotype is rescued if the driver is used alone. Scer\GAL4^repo- or Scer\GAL4^Gli-rL82-driven expression also rescues the invasion phenotype but replaces it with severe morphological anomalies: an elongated nerve cord and flattened brain lobes. Expression of AdamTS-A^UAS.RB controlled by Scer\GAL4^NP577 does not rescue the neural invasion.

(Skeath et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

AdamTS-A^Scer\UAS.RB

AdamTS-A^UAS.RB

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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