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FB2026_01
,
released March 12, 2026
Netrin, CT29512
secreted factors that provide instructive guidance cues in axonogenesis
Please see the JBrowse view of Dmel\NetB for information on other features
To submit a correction to a gene model please use the Contact FlyBase form
AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Gene model reviewed during 5.45
Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
8.3 (northern blot)
793 (aa)
Binds to unc-5 and fra receptors.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\NetB using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Comment: cyclical, peak ZT21-0
Comment: anlage in statu nascendi
Comment: expressed in tin-positive cardioblasts
Comment: lower levels
NetB mRNA is detected in late stage 16 embryos in cardioblasts in the posterior, beating portion of the dorsal vessel (heart proper)
and also, at lower levels within the aorta.
Expressed cyclically in the adult fat body.
At embryonic stage 10, NetB is expressed in many cells within the medial and ventral neurectoderm as well as in many neuroblasts. By late stage 11, expression has become considerably weaker and restricted to single neuroblasts identified as NB6-2 and NB7-2.
NetB RNA is first detected in the developing CNS in early stage 12 embryos at which time it is observed in a subset of the cells along the midline and in a few cells lateral to the midline. In mid stage 12, expression decreases in the lateral cells and increases in the midline cells. By stage 13, it appears that the midline expression is limited to the midline glia. Midline expression continues to the end of embryogenesis.
NetB expression is first observed at the cellular blastoderm stage in the presumptive mesoderm and persists there through gastrulation and then fades. It continues to be expressed weakly in the visceral mesoderm and in small patches in the somatic mesoderm. NetB is expressed strongly in the ventral midline at stage 12 and 13 in the midline glia. It is also expressed in a dynamic pattern in subsets of CNS neurons and is expressed in many more neurons than is NetA. Among these are the MP neurons. NetB is expressed by dorsal muscle and by ventral muscles 6 and 7. It is expressed transiently at stages 13 and 14 in the embryonic precursors of the wing and haltere discs. Finally it is expressed in many cells in the brain and in some epidermal structures in the head that may be imaginal discs.
NetB protein is expressed in the CNS starting in stage 12. It is first observed in cells just lateral to the midline and accumulates in midline cells by late stage 12. A variable degree of axon accumulation is observed starting in stage 12. At stage 14, a lateral group of neurons begins to express NetB. NetB expression is first observed in the developing mesoderm during germ band extension. Expression continues in a variety of mesoderm derivatives including the visceral mesoderm, the somatic mesoderm, and the dorsal vessel. Expressio is observed in the developing stomatogastric nervous system in stage 13. During stage 14, expression is apparent in imaginal disc primordia, including cells that will give rise to the eye-antennal, labial, wing, haltere, and genital discs. NetB protein accumulates in the developing PNS, including the chordotonal organs, and in muscles of both the dorsal and ventral groups. Earlier expression is seen in subsets of muscles. Later, expression is seen on motor axons as they synapse with these specific muscle groups.
Comment: expressed in tin-positive cardioblasts
JBrowse - Visual display of RNA-Seq signals
View Dmel\NetB in JBrowse
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
ChEST reveals this is a target of Mef2.
Although the Netrins are expressed in the target of retinal axons, they are not required for retinal projections.
NetA and NetB function as short-range target recognition molecules for specific motoneurons. The relative balance of Sema-2a and the Netrins controls the choice of a specific target and the avoidance of a potential target. NetB and Sema-2a can both be repulsive for certain motor axons. fra is required for Netrin-mediated attraction, but not repulsion.
Relationship to Netrin not known.
NetA and NetB are expressed by subsets of muscles and function in motor axon targetting. Ectopic expression and loss of function analysis in both the CNS and periphery demonstrates the pattern of netrin expression is crucial to correct patterning of axons, providing evidence that Netrins function as instructive rather than simply permissive, guidance cues.
