FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\NetBcd8.UAS
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General Information
Symbol
Dmel\NetBcd8.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0277762
Feature type
allele
Associated gene
Dmel\NetB
Associated Insertion(s)
Carried in Construct
P{UAS-NetB.cd8}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Timofeev et al., 2012)
Progenitor genotype
Carried in construct
P{UAS-NetB.cd8}
Cytology
Description

UAS regulatory sequences drive expression of a full-length NetB sequence with Mmus\Cd8a, Cerulean and Tag:V5 fused downstream.

(Timofeev et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
NetB
Tagged with
Cerulean
Tag:V5
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of NetBcd8.Scer\UAS widely in the medulla neuropil layers using Scer\GAL4ap-md544 results in approximately 27% of Ecol\lacZPD.Rh6-positive R8 axons stalling at the medulla neuropil border, while 8% terminate incorrectly in the M1/M2 layer.

      Expression of NetBcd8.Scer\UAS in a subset of ectopic layers using Scer\GAL4MH502, a driver active in lamina neurons L1/L2, ascending T1 medulla neurons and C2/C3 neurons throughout development, results in 32% of Ecol\lacZPD.Rh6-positive R8 axons stalling at the medulla neuropil border, while 19% stop in the M1/M2 layer.

      Expression of NetBcd8.Scer\UAS in T1 neurons, under the control of Scer\GAL4CG14200-NP1086 does not affect R8 targeting to layer M3.

      (Timofeev et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Contains an A793G amino acid substitution, compared to genomic NetB sequence, although this does not appear to affect function.

      (Timofeev et al., 2012)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      NetBcd8.Scer\UAS
      NetBcd8.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (1)