FB2026_02 , released June 18, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
Cul3, inc
FlyBase ID
FBig0000091625
Interaction Network
Interactions Browser links
Cul3 network
inc network
Reported Interactions
FBrf0217043-1.coIP.WB
Reference
Stavropoulos and Young, 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Cul3 

Cul-3

bait

HA tag

inc 

inc

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Co-transfection of S2 cells with tagged bait and tagged prey constructs.

(Stavropoulos and Young, 2011)
FBrf0219613-2.coIP.WB
Reference
Pfeiffenberger and Allada, 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Cul3 

CUL3

bait

V5 tag

inc 

INC

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Pfeiffenberger and Allada, 2012)
FBrf0233997-1.CH.MW
Reference
Pirone et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
inc 

INC

neutral component
inc 

INC

neutral component
inc 
inc 

INC

neutral component
inc 
Cul3 

Cul3

neutral component
Cul3 
Cul3 

Cul3

neutral component
Cul3 

Cul3

neutral component
Cul3 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
inc 
BTB domain
sufficient binding region
aa 22-125

coordinates relative to inc-PB

Cul3 
N-terminal region
sufficient binding region
aa 20-381

coordinates relative to Cul3-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Pirone et al., 2016)
FBrf0233997-2a.ITC
Reference
Pirone et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cul3 

Cul3

bait
Cul3 
inc 

INC

prey
inc 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
inc 
BTB domain
sufficient binding region
aa 22-125

coordinates relative to inc-PB

Cul3 
N-terminal region
sufficient binding region
aa 20-381

coordinates relative to Cul3-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Pirone et al., 2016)

Kd = 48 nM. Analysis of stoichiometry indicates that five Cul3 monomers bind an inc pentamer.

(Pirone et al., 2016)
FBrf0261537-1.coIP.WB
Reference
Li et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Cul3 

Cul3

bait

FLAG tag

inc 

inc

prey

Myc tag

inc 
inc 
inc 
inc 
inc 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
inc 
BTB domain
sufficient binding region
aa 22-123

coordinates relative to inc-PB

inc 
BTB domain
mutation decreasing interaction
aa 1-21

coordinates relative to inc-PB

inc 
BTB domain residue 47
mutation decreasing interaction
aa 47

F47A; coordinates relative to inc-PB

inc 
BTB domain residue 105
mutation decreasing interaction
aa 105

F105A; coordinates relative to inc-PB

inc 
BTB domain residue 50
mutation decreasing interaction
aa 105

R50E; coordinates relative to inc-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Li et al., 2025)
FBrf0261537-3.coIP.WB
Reference
Li et al., 2025
Description
physical association
Assay
Collection
Source/Stage
organism / maleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
inc 

inc

bait

FLAG tag

Cul3 

Cul3

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

A FlAG-tagged inc transgenic line was used but anti-inc antibody was used for the coimmunoprecipitation.

(Li et al., 2025)

Source was adult males of transgenic fly line; bait and prey produced from tagged transgenic constructs.

(Li et al., 2025)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)