FlyBase Allele Report: Dmel\Atg6[1]

General Information

Symbol

Dmel\Atg6¹

Species

D. melanogaster

Name

FlyBase ID

FBal0288277

Feature type

allele

Associated gene

Dmel\Atg6

Associated Insertion(s)

TI{TI}Atg6¹

Carried in Construct

Also Known As

atg6^Δ1

Key Links

Allele class

amorphic allele - molecular evidence

amorphic allele - genetic evidence

Mutagen

ends-out gene targeting

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Shravage et al., 2013)

amorphic allele - molecular evidence

(Shravage et al., 2013)

Mutagen

ends-out gene targeting

(Shravage et al., 2013)

Progenitor genotype

Associated Insertion(s)

TI{TI}Atg6¹

Cytology

Description

The entire Atg6 open reading frame has been replaced by a w marker.

(Shravage et al., 2013)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates germ cell cancer

modeled by bam^Δ86

(Zhao et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult brain | adult stage | somatic clone - tissue specific

(Kiral et al., 2020)

autolysosome

(Shravage et al., 2013)

autophagosome

(Shravage et al., 2013)

axon | P-stage

(Kiral et al., 2020)

embryonic/larval fat body | larval stage

(Shravage et al., 2013)

embryonic/larval fat body | larval stage | somatic clone

(Shravage et al., 2013)

eye photoreceptor cell | adult stage | somatic clone - tissue specific

(Kiral et al., 2020)

eye photoreceptor cell | P-stage | somatic clone - tissue specific

(Kiral et al., 2020)

filopodium | P-stage | increased number | somatic clone - tissue specific

(Kiral et al., 2020)

gamma Kenyon cell | adult stage | somatic clone

(Issman-Zecharya and Schuldiner, 2014)

retina | adult stage | somatic clone - tissue specific

(Kiral et al., 2020)

synapse | adult stage | increased number | somatic clone - tissue specific

(Kiral et al., 2020)

synapse | P-stage | increased number

(Kiral et al., 2020)

Detailed Description

Statement

Reference

Homozygous adult mushroom body gamma neuron clones show an axon pruning defect.

(Issman-Zecharya and Schuldiner, 2014)

Following four hours of starvation, Atg6¹ mutant larval fat body cells lack the autophagosomes and autophagolysosomes seen in controls.

Atg6¹ mutant somatic clones generated in the fat body exhibit endocytosis defects; endocytic tracer is seen throughout the cytosol of control cells, but remains on the surface in the mutant cells.

Eye imaginal disc tissue isolated from homozygous Atg6¹ animals at the third instar larval stage is normal in size

All homozygous Atg6¹ mutant larvae display a striking melanotic blood cell mass phenotype. Although single melanotic blood cell masses are often present in Atg6¹ mutant larvae, many homozygous mutant animals possess multiple melanotic blood cell masses. Homozygous Atg6¹ mutants contain nearly ten times as many blood cells compared to controls. No masses are seen in heterozygous flies, although they do contain twice as many blood cell as control larvae.

Atg6¹ mutant larvae display enlarged lymph glands between 80 hours and 104 hours after egg lay compared to controls. The animals progressively accumulate blood cell masses over his time period. By 126 hours, all of the Atg6¹ mutant larvae contain numerous ectopic blood cell masses. These blood cell masses initiate melanisation 104 hours after egg lay and continue to do so until 160 hours after egg lay. Most Atg6¹ mutant animals do not pupariate.

The lymph glands are also enlarged in Atg6¹ mutant third instar larvae.

(Shravage et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Atg6¹ has abnormal neuroanatomy | somatic clone phenotype, suppressible | somatic clone by ptc¹⁶

(Issman-Zecharya and Schuldiner, 2014)

NOT suppressed by

Statement

Reference

Atg6¹ has melanotic mass phenotype | recessive | third instar larval stage phenotype, non-suppressible by ref(2)P^od2

(Shravage et al., 2013)

Atg6¹ has melanotic mass phenotype | recessive | third instar larval stage phenotype, non-suppressible by Dif¹

(Shravage et al., 2013)

Atg6¹ has melanotic mass phenotype | recessive | third instar larval stage phenotype, non-suppressible by dl¹⁵

(Shravage et al., 2013)

Atg6¹ has melanotic mass phenotype | recessive | third instar larval stage phenotype, non-suppressible by Rel^E20

(Shravage et al., 2013)

Atg6¹ has melanotic mass phenotype | recessive | third instar larval stage phenotype, non-suppressible by Rel^E20/Dif¹

(Shravage et al., 2013)

Atg6¹ has melanotic mass phenotype | recessive | third instar larval stage phenotype, non-suppressible by Rel^E20/Df(2L)J4/Dif¹

(Shravage et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

Atg6¹ has gamma Kenyon cell | somatic clone phenotype, suppressible | somatic clone by ptc¹⁶

(Issman-Zecharya and Schuldiner, 2014)

Suppressor of

Statement

Reference

Atg6¹ is a suppressor of eye disc | somatic clone | cell non-autonomous phenotype of Vps25^N55

(Shravage et al., 2013)

Atg6¹ is a suppressor of eye disc | somatic clone | cell non-autonomous phenotype of shrb^G5

(Shravage et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

The axon pruning defect of Atg6¹ mushroom body gamma neuron clones is significantly suppressed if the neurons are also homozygous for ptc¹⁶.

(Issman-Zecharya and Schuldiner, 2014)

Atg6¹ suppresses the non-autonomous overgrowth seen in Vps25^N55 mutant eye disc clones.

Atg6¹ suppresses the non-autonomous overgrowth seen in shrb^G5 mutant eye disc clones.

ref(2)P^od2 fails to suppress the melanotic mass phenotype seen in Atg6¹ mutant third instar larvae.

Dif¹ fails to suppress the melanotic mass phenotype seen in Atg6¹ mutant third instar larvae.

dl¹⁵ fails to suppress the melanotic mass phenotype seen in Atg6¹ mutant third instar larvae.

Rel^E20 fails to suppress the melanotic mass phenotype seen in Atg6¹ mutant third instar larvae.

A Dif¹ Rel^E20 double mutant background fails to suppress the melanotic mass phenotype seen in Atg6¹ mutant third instar larvae.

A Dif¹/Df(2L)J4 Rel^E20 mutant background fails to suppress the melanotic mass phenotype seen in Atg6¹ mutant third instar larvae.

(Shravage et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

Atg6⁰⁰⁰⁹⁶

(Shravage et al., 2013)

Rescued by

Atg6¹ is rescued by Scer\GAL4^Act.PU/Atg6^UAS.GFP

(Shravage et al., 2013)

Partially rescued by

Atg6¹ is partially rescued by Scer\GAL4^GMR.PU/Atg6^{UAS.ORF.GW.Tag:HA}

(Kiral et al., 2020)

Comments

Expression of Atg6^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^Act.PU completely rescues the melanotic mass phenotype seen in homozygous Atg6¹ mutant third instar larvae.

(Shravage et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Atg6¹

(Issman-Zecharya and Schuldiner, 2014, Chang et al., 2013, Shravage et al., 2013)

atg6 (Δ)

(Shen et al., 2021)

atg6¹

(Kiral et al., 2020, Jain et al., 2015)

atg6^Δ1

(Lin et al., 2021, Zhao et al., 2018)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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