Homozygous adult mushroom body gamma neuron clones show an axon pruning defect.
Following four hours of starvation, Atg61 mutant larval fat body cells lack the autophagosomes and autophagolysosomes seen in controls.
Atg61 mutant somatic clones generated in the fat body exhibit endocytosis defects; endocytic tracer is seen throughout the cytosol of control cells, but remains on the surface in the mutant cells.
Eye imaginal disc tissue isolated from homozygous Atg61 animals at the third instar larval stage is normal in size
All homozygous Atg61 mutant larvae display a striking melanotic blood cell mass phenotype. Although single melanotic blood cell masses are often present in Atg61 mutant larvae, many homozygous mutant animals possess multiple melanotic blood cell masses. Homozygous Atg61 mutants contain nearly ten times as many blood cells compared to controls. No masses are seen in heterozygous flies, although they do contain twice as many blood cell as control larvae.
Atg61 mutant larvae display enlarged lymph glands between 80 hours and 104 hours after egg lay compared to controls. The animals progressively accumulate blood cell masses over his time period. By 126 hours, all of the Atg61 mutant larvae contain numerous ectopic blood cell masses. These blood cell masses initiate melanisation 104 hours after egg lay and continue to do so until 160 hours after egg lay. Most Atg61 mutant animals do not pupariate.
The lymph glands are also enlarged in Atg61 mutant third instar larvae.