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General Information
Symbol
Dmel\Atg61
Species
D. melanogaster
Name
FlyBase ID
FBal0288277
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

The entire Atg6 open reading frame has been replaced by a w marker.

Insertion components
TI{TI}Atg61
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Homozygous adult mushroom body gamma neuron clones show an axon pruning defect.

Following four hours of starvation, Atg61 mutant larval fat body cells lack the autophagosomes and autophagolysosomes seen in controls.

Atg61 mutant somatic clones generated in the fat body exhibit endocytosis defects; endocytic tracer is seen throughout the cytosol of control cells, but remains on the surface in the mutant cells.

Eye imaginal disc tissue isolated from homozygous Atg61 animals at the third instar larval stage is normal in size

All homozygous Atg61 mutant larvae display a striking melanotic blood cell mass phenotype. Although single melanotic blood cell masses are often present in Atg61 mutant larvae, many homozygous mutant animals possess multiple melanotic blood cell masses. Homozygous Atg61 mutants contain nearly ten times as many blood cells compared to controls. No masses are seen in heterozygous flies, although they do contain twice as many blood cell as control larvae.

Atg61 mutant larvae display enlarged lymph glands between 80 hours and 104 hours after egg lay compared to controls. The animals progressively accumulate blood cell masses over his time period. By 126 hours, all of the Atg61 mutant larvae contain numerous ectopic blood cell masses. These blood cell masses initiate melanisation 104 hours after egg lay and continue to do so until 160 hours after egg lay. Most Atg61 mutant animals do not pupariate.

The lymph glands are also enlarged in Atg61 mutant third instar larvae.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement
Reference
Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The axon pruning defect of Atg61 mushroom body gamma neuron clones is significantly suppressed if the neurons are also homozygous for ptc16.

Atg61 suppresses the non-autonomous overgrowth seen in Vps25N55 mutant eye disc clones.

Atg61 suppresses the non-autonomous overgrowth seen in shrbG5 mutant eye disc clones.

ref(2)Pod2 fails to suppress the melanotic mass phenotype seen in Atg61 mutant third instar larvae.

Dif1 fails to suppress the melanotic mass phenotype seen in Atg61 mutant third instar larvae.

dl15 fails to suppress the melanotic mass phenotype seen in Atg61 mutant third instar larvae.

RelE20 fails to suppress the melanotic mass phenotype seen in Atg61 mutant third instar larvae.

A Dif1 RelE20 double mutant background fails to suppress the melanotic mass phenotype seen in Atg61 mutant third instar larvae.

A Dif1/Df(2L)J4 RelE20 mutant background fails to suppress the melanotic mass phenotype seen in Atg61 mutant third instar larvae.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of Atg6Scer\UAS.T:Avic\GFP under the control of Scer\GAL4Act.PU completely rescues the melanotic mass phenotype seen in homozygous Atg61 mutant third instar larvae.

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Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (4)