ref(2)Pod2/ref(2)Pod3 mutant midgut cells at 2hr after puparium formation show similar amount of mitochondria as in controls.
Embryos derived from ref(2)Pod2/ref(2)Pod3 females show a moderate delay in the clearance of the paternal mitochondrial derivatives compared to wild type.
Early onion-stage spermatids of mutant males have abnormal nebenkern (mitochondrial derivatives) which envelop the white nuclei instead of being adjacent to them. The mutant spermatids undergo normal elongation, but individualisation is abnormal. There is an increased distance between the axoneme and mitochondrial derivative than normal in post-individualisation cysts, and the mitochondrial derivative contains a large mass of electron-dense material.
The density of mitochondrial DNA nucleoids in the indirect flight muscle is increased in mutant flies compared to wild type.
Mutants show an increase in mitochondrial DNA heteroplasmy compared to wild type in both 3 day old and 25 day old adults.
Mutant animals show a decrease in lifespan compared to wild type, both on normal food, or on food supplemented with either rotenone or paraquat. Locomotor activity is impaired from a young age and progresses with age.
Mutants show mitochondrial defects in the indirect flight muscles.