ref(2)Pod2/ref(2)Pod3 mutant midgut cells at 2hr after puparium formation show similar amount of mitochondria as in controls.
Embryos derived from ref(2)Pod2/ref(2)Pod3 females show a moderate delay in the clearance of the paternal mitochondrial derivatives compared to wild type.
Early onion-stage spermatids of mutant males have abnormal nebenkern (mitochondrial derivatives) which appear unusually pale. The overall architecture of post-individualisation cysts is completely disorganised in the mutant males. Axonemes are visible, but the mitochondrial derivatives are frequently smaller than normal, containing electron-dense material and showing a striking vacuolisation.
The density of mitochondrial DNA nucleoids in the indirect flight muscle is increased in mutant flies compared to wild type.
Mutants show an increase in mitochondrial DNA heteroplasmy compared to wild type in both 3 day old and 25 day old adults.
Mutant animals show a decrease in lifespan compared to wild type, both on normal food, or on food supplemented with either rotenone or paraquat. Locomotor activity is impaired from a young age and progresses with age.
Mutants show mitochondrial defects in the indirect flight muscles.