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General Information
Symbol
Dmel\ref(2)Pod3
Species
D. melanogaster
Name
FlyBase ID
FBal0032568
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Comment:

Approximate location of small deletion. Leads to nonsense mutation.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Short deletion in the coding region, in exon 2.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

ref(2)Pod2/ref(2)Pod3 mutant midgut cells at 2hr after puparium formation show similar amount of mitochondria as in controls.

Embryos derived from ref(2)Pod2/ref(2)Pod3 females show a moderate delay in the clearance of the paternal mitochondrial derivatives compared to wild type.

Early onion-stage spermatids of mutant males have abnormal nebenkern (mitochondrial derivatives) which appear unusually pale. The overall architecture of post-individualisation cysts is completely disorganised in the mutant males. Axonemes are visible, but the mitochondrial derivatives are frequently smaller than normal, containing electron-dense material and showing a striking vacuolisation.

The density of mitochondrial DNA nucleoids in the indirect flight muscle is increased in mutant flies compared to wild type.

Mutants show an increase in mitochondrial DNA heteroplasmy compared to wild type in both 3 day old and 25 day old adults.

Mutant animals show a decrease in lifespan compared to wild type, both on normal food, or on food supplemented with either rotenone or paraquat. Locomotor activity is impaired from a young age and progresses with age.

Mutants show mitochondrial defects in the indirect flight muscles.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference

ref(2)Pod3 has male sterile phenotype, suppressible by Su(P)GlSbH/Su(P)[+]

ref(2)Pod3 has male sterile phenotype, suppressible by Su(P)[+]/Su(P)TW2

ref(2)Pod3 has male sterile phenotype, suppressible by Su(P)[+]/Su(P)db

ref(2)Pod3 has male sterile phenotype, suppressible by Su(P)hd48/Su(P)[+]

ref(2)Pod3 has male sterile phenotype, suppressible by Su(P)hd5/Su(P)[+]

ref(2)Pod3 has male sterile phenotype, suppressible by Su(P)pe/Su(P)[+]

ref(2)Pod3 has male sterile phenotype, suppressible by Su(P)pr26/Su(P)[+]

ref(2)Pod3 has male sterile phenotype, suppressible by Su(P)[+]/Su(P)TW9

Phenotype Manifest In
Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The mitochondrial clustering seen in the indirect flight muscles of Pink1B9 flies is significantly suppressed by ref(2)Pod3.

Expression of parkScer\UAS.cGa under the control of Scer\GAL4da.G32 significantly suppresses the penetrance of the indented thorax phenotype seen in Pink1B9 mutants. This suppression is reduced if the flies are also carrying ref(2)Pod3.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (10)