Two-hybrid system: yeast lexA-BD/GAL4-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was live HEK293T cells; proteins produced from transfected constructs.
Two-hybrid system: yeast LexA-BD/Gal4-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
